NR5A2 / CPF antibody

Principal name

NR5A2 / CPF antibody

Alternative names for NR5A2 / CPF antibody

B1F, FTF, Alpha-1-Fetoprotein transcription factor, AFP Receptor, Nuclear receptor subfamily 5 group A member 2, Hepatocytic transcription factor, B1-binding factor, CYP7A promoter-binding factor, Liver receptor homolog 1

SwissProt ID

O00482 (Human), P45448 (Mouse), Q9QWM1 (Rat)

Gene ID

2494 (NR5A2), 174 (AFP), 26424 (Nr5a2), 60349

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Eq (Equine), Hst (Hamster), Ms (Mouse), Rt (Rat), Xen (Xenopus), Ze (Zebrafish), Bov (Bovine), African clawed frog, Por (Porcine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Frozen Sections (C), ELISA (capture) (E(capture)), Functional assay (FN), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of NR5A2 / CPF antibody

Alpha1-fetoprotein transcription factor (Ftf), a NR5 fushi tarazu-like receptor, also is known as "human B1-binding factor" (HB1F), "CYP7A promoter-binding factor" (CPF), and "liver receptor homolog 1" (LRH1). Ftf has been shown to affect the transcription of hepatic genes including alpha fetoprotein encoding gene (AFP), viral hepatitis B enhancer II, and 7-alpha-hydroxylase encoding gene (CYP7A1). Ftf has also been shown to affect hepatic cholesterol metabolism and bile acid synthesis.
Ftf has been shown to regulate aromatase activities in adipose tissue, and alterations in Ftf expression and/or activity in adipose tissue could have considerable effects on local estrogen production and breast cancer development. LRH has been shown to expressly strongly in gonadal tissues, and may play an important role in the regulation of gonadal function.
Three isoforms of Ftf have been identified: wild type (495 aa), CPF variant 1 (contains a 46-aa insertion in the A/B domain), and CPF variant 2 (contains a 172-aa deletion within the D/E domain).
Ftf expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

General readings

Lee,Y.K., (2006) J. Biol. Chem. 281 (12), 7850-7855

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NR5A2 / CPF antibody

TA326727 (1µg/ml) staining of HepG2 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-CPF antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6066 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

WB Suggested Anti-Nr5a2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Lung Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

WB Suggested Anti-Nr5a2 Antibody Titration: 0.2-1 ug/ml; Positive Control: SP2/0 cell lysate Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

WB Suggested Anti-NR5A2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

Western blot analysis of extracts of various cell lines, using NR5A2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

NR5A2 / CPF (Ligand Binding Dom.) antibody

Anti-NR5A2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Can, Chk, Elephant, Eq, Fi, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rt, Sh, Tr, Xen P
50 µg / €379.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

Anti-LRH-1 / NR5A2 antibody IHC of human adipocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 7 ug/ml. Rabbit Purified Hu, Xen
50 µg / €379.00
  OriGene Technologies, Inc.

NR5A2 / CPF (Internal region) antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Platypus, Prim, Rt, Sh, Tr, Xen P
50 µg / €379.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

WB Suggested Anti-NR5A2 Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, GP, Hu, Por, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

Host: Rabbit; Target Name: Nr5a2; Sample Tissue: Rat Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

NR5A2 / CPF antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NR5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805074) Mouse IgG1 OTI3H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NR5A2 / CPF antibody

NR5A2 / CPF Mouse IgG2a 5E1 Purified Hu ICC/IF, WB
1 mg / €490.00
  Acris Antibodies GmbH

NR5A2 / CPF (1-67) antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

NR5A2 / CPF antibody

NR5A2 / CPF Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

NR5A2 / CPF antibody

NR5A2 / CPF Mouse IgG2a 2B8 Hu E, WB
0.2 mg / €400.00
  Abnova Taiwan Corp.

NR5A2 / CPF antibody

NR5A2 / CPF Mouse IgG2a 5E1 Hu C, E, WB
0.2 mg / €400.00
  Abnova Taiwan Corp.

NR5A2 / CPF antibody

Human HepG2; WB Suggested Anti-NR5A2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; NR5A2 antibody - middle region (ARP32524_P050) in Human HepG2 cells using Western Blot Rabbit Purified African clawed frog, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NR5A2 / CPF (C-term) antibody

Mouse Lung; WB Suggested Anti-Nr5a2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Lung; Nr5a2 antibody - C-terminal region (ARP37407_P050) in Mouse Lung cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

NR5A2 / CPF (Center) antibody

Mouse SP2/0; WB Suggested Anti-Nr5a2 Antibody Titration: 0.2-1 ug/ml. Positive Control: SP2/0 cell lysate; Nr5a2 antibody - middle region (ARP37408_P050) in Mouse SP2/0 cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

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