NRAS antibody

Principal name

NRAS antibody

Alternative names for NRAS antibody

GTPase NRas, N-Ras, HRAS1, ALPS4, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog

SwissProt ID

P01111 (Human), P08556 (Mouse), P12825 (Cavpo), P79737 (Danre), Q04970 (Rat), Q2MJK3 (Pig), Q5F352 (Chick), Q91806 (Xenla)

Gene ID

4893 (NRAS)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Dros (Drosophila), African clawed frog, Xen (Xenopus), Chk (Chicken), Bov (Bovine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of NRAS antibody

Ras proteins function as GDP/GTP-regulated binary switches in signal transduction cascades that can lead to cell growth, proliferation, differentiation, or survival. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. This Ras superfamily has at least five major branches that include Ras, Rho, Ran, Arf/Sar, Rab. Ras itself has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. They are collectively referred to as Ras. In its active form, Ras is bound to GTP. This causes a conformational change that allow it to interact and bind to several effector molecules, most notably the members of the Raf family, the RalGDS family, and Phosphoinositide 3-kinases (PI3 Kinase). Ras then cleaves GTP to GDP resulting in its inactivation. In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways.

General readings

1. Thomas NE, Edmiston SN, Alexander A, Millikan RC, Groben PA, Hao H, Tolbert D, Berwick M, Busam K, Begg CB, Mattingly D, Ollila DW, Tse CK, Hummer A, Lee-Taylor J, Conway K. Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma. Cancer Epidemiol Biomarkers Prev. 2007 May;16(5):991-7.
PMID: 17507627

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NRAS (Q61R) antibody

SK-MEL-2 cells stained with anti-N-Ras (Q61R) antibody Rabbit IgG SP174 Purified Hu P, WB
0.5 ml / €630.00
  Acris Antibodies GmbH

NRAS (Q61R) antibody

SK-MEL-2 cells stained with anti-N-Ras (Q61R) antibody Rabbit IgG SP174 Purified Hu P, WB
1 ml / €840.00
  Acris Antibodies GmbH

NRAS (Q61R) antibody

SK-MEL-2 cells stained with anti-N-Ras (Q61R) antibody Rabbit IgG SP174 Purified Hu P, WB
0.1 ml / €410.00
  Acris Antibodies GmbH

NRAS antibody

TA302554 (0.01µg/ml) staining of A431 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NRAS (N-term) antibody

Western blot analysis of anti-NRAS Antibody (N-term)(Cat.#TA324982) in CEM cell line lysates (35ug/lane). NRAS(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NRAS (C-term) antibody

NRAS Antibody (C-term) (Cat. #TA324983) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NRAS antibody

NRAS Rabbit Purified Bov, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NRAS antibody

Western blot analysis of N-RAS in 293 cell lysate with N-RAS antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NRAS antibody

NRAS Antibody (N-term) (Cat. #TA328115) western blot analysis in A431 and rat C6 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow). Mouse IgG1 822CT17.5.1 Purified Chk, Hu, Ms, Por, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

NRAS antibody

Western blot analysis on HeLa cell lysate using RASH/RASK/RASN Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NRAS antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505835) Mouse IgG1 OTI5G7 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NRAS antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NRAS Rabbit Polyclonal antibody. (TA590133) Rabbit IgG Purified Hu E, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NRAS (167-179) antibody

IHC Image: Small intestine Goat IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, GP, Hst, Hu, Mky, Ms, Op, Por, Prim, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NRAS antibody

NRAS Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

NRAS antibody

Detection limit for recombinant GST tagged NRAS is approximately 0.1ng/ml as a capture antibody. Mouse IgG 2A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NRAS antibody

Western blot analysis of NRAS ( arrow ) using rabbit NRAS polyclonal antibody ( Cat # PAB3461 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NRAS gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NRAS antibody

NRAS polyclonal antibody ( Cat # PAB7450 ) ( 2.5 µg/ml ) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu, Rt E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NRAS antibody

candida albicans Ras1P; . Lanes: Lane 1: 250ng candida albicans Ras1P protein. Primary Antibody Dilution: 1:5000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:10,000. Gene Name: KRAS. Submitted by: Dr. Alexandra Oliveira, Instituto de Biologia Molecular e Celular.; KRAS antibody - N-terminal region (ARP55408_P050) in candida albicans Ras1P cells using Western Blot Rabbit Aff - Purified African clawed frog, Dros, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

NRAS antibody

ELISA: NRAS Antibody [23450002] Rabbit Hu E, P, WB
50 µg / €400.00
  Novus Biologicals Inc.

NRAS antibody

Western Blot: Ras Antibody (EP1125Y) [NB100-80042] - Western blot analysis on C6 cell lysate using anti-Ras, dilution 1:500,000. Rabbit EP1125Y Purified Hu, Ms, Rt F, IP, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

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