NSF antibody

Principal name

NSF antibody

Alternative names for NSF antibody

Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Vesicular-fusion protein NSF

SwissProt ID

P46459 (Human), P46460 (Mouse), Q9QUL6 (Rat)

Gene ID

4905 (NSF), 60355 (Nsf), 18195

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Bov (Bovine), Chk (Chicken), Can (Canine), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Frozen Sections (C)

Background of NSF antibody

The N-ethylmaleimide-sensitive fusion protein (NSF), acting in concert with SNAPs (Soluble NSF attachment proteins) and SNARES (SNAP receptors), is required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-toplasma membrane transport. In its functional form, NSF is a homotrimer of 76-kDa subunits. Each subunit contains an amino-terminal domain (N) and two homologous ATP-binding domains (D1 and D2). Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (a- or b-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NSF antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NSF antibody

Western blot analysis of extracts from CoLo cells, using NSF antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NSF antibody

Figure 1. A typical Western blot (10% SDS-PAGE) of 3T3 (Lane 1), PC12 (Lane 2), MDBK (Lane 3) and normal human fibroblasts (Lane 4) probed with clone NSF-1 anti-NSF at 0.5 mg/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown). Mouse IgG1 NSF-1 Purified Bov, Chk, Hu, Ms, Rt E, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

NSF control peptide

NSF Purified
50 µg / €190.00
  Acris Antibodies GmbH

NSF antibody

NSF Rabbit Aff - Purified Ms, Rt IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

NSF (C-term) antibody

Figure 1. Western blot of rat caudate lysate showing specific labeling of the ~75k NSF protein. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

NSF antibody

Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Ms, Rt IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NSF antibody

Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T01) by NSF MaxPab polyclonal antibody.

Lane 1: NSF transfected lysate(81.84 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

NSF antibody

Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T01) by NSF MaxPab polyclonal antibody.

Lane 1: NSF transfected lysate(81.84 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

NSF antibody

Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T02) by NSF MaxPab polyclonal antibody.

Lane 1: NSF transfected lysate(82.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NSF antibody

Western blot analysis of 3T3 (lane 1), PC-12 (lane 2), MDBK (lane 3) and normal human fibroblasts (lane 4) probed with NSF monoclonal antibody, clone NSF-1 ( Cat # MAB1966 ) at 0.5 µg/ml. Bov, Chk, Hu, Ms, Rt
0.1 mg / €390.00
  Abnova Taiwan Corp.

NSF antibody

NSF Rabbit Hu, Ms, Rt C, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NSF antibody

Western blot of rat caudate lysate showing specific labeling of the ~75k Nsf protein. Using Nsf polyclonal antibody ( Cat # PAB9620 ). Rabbit Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

NSF antibody

Mouse brain stem; . Sample Type :. Mouse brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. NSF: Red DAPI:Blue. Gene Name :. NSF. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; NSF antibody - C-terminal region (ARP40629_P050) in Mouse brain stem cells using Immunohistochemistry Rabbit Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NSF antibody

Western blot analysis of NSF expression in MCF7 (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NSF antibody

Western blot analysis of NSF expression in MCF7 (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NSF antibody

Western blot analysis of NSF expression in MCF7 (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NSF

NSF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NSF

NSF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NSF

NSF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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