NSF antibody

Principal name

NSF antibody

Alternative names for NSF antibody

Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Vesicular-fusion protein NSF

SwissProt ID

P46459 (Human), P46460 (Mouse), Q9QUL6 (Rat)

Gene ID

4905 (NSF), 60355 (Nsf), 18195

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Bov (Bovine), Chk (Chicken), Can (Canine), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Frozen Sections (C)

Background of NSF antibody

The N-ethylmaleimide-sensitive fusion protein (NSF), acting in concert with SNAPs (Soluble NSF attachment proteins) and SNARES (SNAP receptors), is required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-toplasma membrane transport. In its functional form, NSF is a homotrimer of 76-kDa subunits. Each subunit contains an amino-terminal domain (N) and two homologous ATP-binding domains (D1 and D2). Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (a- or b-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.

  1. Quick-Filters:
  2. Highly Validated (1)

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NSF antibody

  Rabbit Polyclonal NSF Antibody  
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NSF antibody

  Rabbit polyclonal anti-NSF antibody  
Western blot analysis of extracts from CoLo cells, using NSF antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NSF antibody

Figure 1. A typical Western blot (10% SDS-PAGE) of 3T3 (Lane 1), PC12 (Lane 2), MDBK (Lane 3) and normal human fibroblasts (Lane 4) probed with clone NSF-1 anti-NSF at 0.5 mg/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown). Mouse IgG1 NSF-1 Purified Bov, Chk, Hu, Ms, Rt E, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

NSF control peptide

NSF Purified
50 µg / €180.00
  Acris Antibodies GmbH

NSF antibody

NSF Rabbit Aff - Purified Ms, Rt IP, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

NSF (C-term) antibody

Figure 1. Western blot of rat caudate lysate showing specific labeling of the ~75k NSF protein. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

NSF antibody

Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Ms, Rt IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Nsf - mouse gene knockout kit via CRISPR

Nsf - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Nsf - mouse gene knockout kit via CRISPR

Nsf - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nsf - mouse gene knockout kit via CRISPR

Nsf - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nsf - mouse gene knockout kit via CRISPR

Nsf - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NSF

  Recombinant protein of human N-ethylmaleimide-sensitive factor (NSF)  
NSF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nsf (GFP-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf), 200 uL, >10^7 TU/mL

Lenti ORF particles, Nsf (GFP-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf), 200 uL, >10^7 TU/mL  
200 µl / €1,440.00
  OriGene Technologies, Inc.

Lenti ORF particles, Nsf (Myc-DDK-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf), 200 uL, >10^7 TU/mL

Lenti ORF particles, Nsf (Myc-DDK-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf), 200 uL, >10^7 TU/mL  
200 µl / €1,290.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nsf (GFP-tagged ORF) - Rat N-ethylmaleimide-sensitive factor (Nsf), 200 uL, >10^7 TU/mL

Lenti ORF particles, Nsf (GFP-tagged ORF) - Rat N-ethylmaleimide-sensitive factor (Nsf), 200 uL, >10^7 TU/mL  
200 µl / €1,440.00
  OriGene Technologies, Inc.

Lenti ORF particles, Nsf (Myc-DDK-tagged ORF) - Rat N-ethylmaleimide-sensitive factor (Nsf), 200 uL, >10^7 TU/mL

Lenti ORF particles, Nsf (Myc-DDK-tagged ORF) - Rat N-ethylmaleimide-sensitive factor (Nsf), 200 uL, >10^7 TU/mL  
200 µl / €1,294.00
  OriGene Technologies, Inc.

16 Item(s)

per page
  • LinkedIn