NSF antibody

Principal name

NSF antibody

Alternative names for NSF antibody

Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Vesicular-fusion protein NSF

SwissProt ID

P46459 (Human), P46460 (Mouse), Q9QUL6 (Rat)

Gene ID

4905 (NSF), 60355 (Nsf), 18195, 6384905 (mpg)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Bov (Bovine), Can (Canine), Hst (Hamster), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of NSF antibody

N ethylmaleimide sensitive factor (NSF) is an approx. 83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NSF antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NSF antibody

Western blot analysis of extracts from CoLo cells, using NSF antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NSF antibody

Figure 1. A typical Western blot (10% SDS-PAGE) of 3T3 (Lane 1), PC12 (Lane 2), MDBK (Lane 3) and normal human fibroblasts (Lane 4) probed with clone NSF-1 anti-NSF at 0.5 mg/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown). Mouse IgG1 NSF-1 Purified Bov, Chk, Hu, Ms, Rt E, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

NSF control peptide

NSF Purified
50 µg / €190.00
  Acris Antibodies GmbH

NSF antibody

NSF Rabbit Aff - Purified Ms, Rt IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

NSF (C-term) antibody

Figure 1. Western blot of rat caudate lysate showing specific labeling of the ~75k NSF protein. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

NSF antibody

Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Ms, Rt IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NSF antibody

Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T01) by NSF MaxPab polyclonal antibody.

Lane 1: NSF transfected lysate(81.84 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

NSF antibody

Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T01) by NSF MaxPab polyclonal antibody.

Lane 1: NSF transfected lysate(81.84 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

NSF antibody

Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T02) by NSF MaxPab polyclonal antibody.

Lane 1: NSF transfected lysate(82.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

NSF antibody

Western blot analysis of 3T3 (lane 1), PC-12 (lane 2), MDBK (lane 3) and normal human fibroblasts (lane 4) probed with NSF monoclonal antibody, clone NSF-1 ( Cat # MAB1966 ) at 0.5 µg/ml. Bov, Chk, Hu, Ms, Rt
0.1 mg / €390.00
  Abnova Taiwan Corp.

NSF antibody

NSF Rabbit Hu, Ms, Rt C, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NSF antibody

Western blot of rat caudate lysate showing specific labeling of the ~75k Nsf protein. Using Nsf polyclonal antibody ( Cat # PAB9620 ). Rabbit Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

NSF antibody

Mouse brain stem; . Sample Type :. Mouse brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. NSF: Red DAPI:Blue. Gene Name :. NSF. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; NSF antibody - C-terminal region (ARP40629_P050) in Mouse brain stem cells using Immunohistochemistry Rabbit Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

NSF antibody

Western blot analysis of NSF expression in MCF7 (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

NSF antibody

Western blot analysis of NSF expression in MCF7 (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

NSF antibody

Western blot analysis of NSF expression in MCF7 (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

NSF antibody

ELISA: NSF Antibody [19750002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

NSF antibody

Western Blot: NSF Antibody [NB300-733] - Rat caudate lysate showing specific labeling of the ~75k NSF protein. Rabbit Aff - Purified Rt WB
0.1 ml / €380.00
  Novus Biologicals Inc.

NSF antibody

NSF Mouse IgG2b 9G7-3 Purified Bov, Can, Chk, Hu, Mky, Ms, Por, Rb, Rt, Sh WB
50 µg / €380.00
  Novus Biologicals Inc.

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