NSUN2 antibody

Principal name

NSUN2 antibody

Alternative names for NSUN2 antibody

SAKI, TRM4, tRNA (cytosine-5-)-methyltransferase NSUN2, NOL1/NOP2/Sun domain family member 2, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, Substrate of AIM1/Aurora kinase B

SwissProt ID

Q08J23 (Human), Q1HFZ0 (Mouse), Q9W4M9 (Drome)

Gene ID

54888 (NSUN2), 28114 (Nsun2)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NSUN2 antibody

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

General readings

Huttenhofer,A., (2007) Mol. Biol. Cell 18 (3), 1107-1117

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NSUN2 antibody

  Rabbit Polyclonal Anti-NSUN2 Antibody  
WB Suggested Anti-NSUN2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NSUN2 antibody

  Rabbit polyclonal anti-NSun2 (Misu) antibody  
NSUN2 Rabbit IgG Purified Hu ICC/IF, IP, WB
100 µl / €325.00
  OriGene Technologies, Inc.

NSUN2 (Center) antibody

NSUN2 Antibody (Center) (Cat. #AP52942PU-N) western blot analysis in mouse spleen tissue lysates (35µg/lane).This demonstrates the NSUN2 antibody detected the NSUN2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NSUN2 antibody

NSUN2 Rabbit Purified Ms C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

NSUN2 antibody

Human HeLa; WB Suggested Anti-NSUN2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate; NSUN2 antibody - C-terminal region (ARP48811_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

NSUN2 - human gene knockout kit via CRISPR

NSUN2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NSUN2 - human gene knockout kit via CRISPR

NSUN2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NSUN2 - human gene knockout kit via CRISPR

NSUN2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NSUN2 - human gene knockout kit via CRISPR

NSUN2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nsun2 - mouse gene knockout kit via CRISPR

Nsun2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Nsun2 - mouse gene knockout kit via CRISPR

Nsun2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nsun2 - mouse gene knockout kit via CRISPR

Nsun2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nsun2 - mouse gene knockout kit via CRISPR

Nsun2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NSUN2

  Recombinant protein of human NOL1/NOP2/Sun domain family, member 2 (NSUN2)  
NSUN2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

NSUN2 overexpression lysate

NSUN2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NSUN2 (mGFP-tagged) - Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, NSUN2 (mGFP-tagged) - Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, NSUN2 (Myc-DDK tagged) - Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, NSUN2 (Myc-DDK tagged) - Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nsun2 (GFP-tagged) - Mouse NOL1/NOP2/Sun domain family member 2 (Nsun2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Nsun2 (GFP-tagged) - Mouse NOL1/NOP2/Sun domain family member 2 (Nsun2), 200 uL, >10^7 TU/mL  
200 µl / €1,460.00
  OriGene Technologies, Inc.

Lenti ORF particles, Nsun2 (Myc-DDK-tagged) - Mouse NOL1/NOP2/Sun domain family member 2 (Nsun2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Nsun2 (Myc-DDK-tagged) - Mouse NOL1/NOP2/Sun domain family member 2 (Nsun2), 200 uL, >10^7 TU/mL  
200 µl / €1,310.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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