NSUN2 antibody

Principal name

NSUN2 antibody

Alternative names for NSUN2 antibody

SAKI, TRM4, tRNA (cytosine-5-)-methyltransferase NSUN2, NOL1/NOP2/Sun domain family member 2, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, Substrate of AIM1/Aurora kinase B

SwissProt ID

Q08J23 (Human), Q1HFZ0 (Mouse), Q9W4M9 (Drome)

Gene ID

54888 (NSUN2), 28114 (Nsun2)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C)

Background of NSUN2 antibody

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

General readings

Huttenhofer,A., (2007) Mol. Biol. Cell 18 (3), 1107-1117

5 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NSUN2 (Center) antibody

NSUN2 Antibody (Center) (Cat. #AP52942PU-N) western blot analysis in mouse spleen tissue lysates (35µg/lane).This demonstrates the NSUN2 antibody detected the NSUN2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NSUN2 antibody

NSUN2 Rabbit Purified Ms C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

NSUN2 antibody

Human HeLa; WB Suggested Anti-NSUN2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate; NSUN2 antibody - C-terminal region (ARP48811_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NSUN2

NSUN2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

NSUN2 overexpression lysate

NSUN2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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