NT5C2 antibody

Principal name

NT5C2 antibody

Alternative names for NT5C2 antibody

NT5B, NT5CP, PNT5, Cytosolic purine 5'-nucleotidase

SwissProt ID

O46411 (Bovin), P49902 (Human), Q3V1L4 (Mouse)

Gene ID

22978 (NT5C2), 76952 (Nt5c2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP)

Background of NT5C2 antibody

Various cytotoxic nucleoside analogs (NA) have been employed in the treatment of hematologic malignancies. These NA include pyrimidine analog cytosine arabinoside (ara-c) and purine analogs, cladribine and fludarabine. These NA become therapeutically effective only after phosporylation to the triphosphate level. The 5’-nucleotidases (5’-NTs) dephosphorylate the monophosphate form of NA and, therefore, may affect the pharmacological activity of these antimetabolites. Several 5’-NTs attached to membranes or present in the cytosol or in mitochondri. There are two cytosolic cyclic 5’nucleotidases are cN-II and dNT-1 and one mitochondrial (dNT-2) nucleotidase known to be present in the cells. cN-I plays a significant role in AMP breakdown to adenosine whereas cN-II breaks down IMP to inosine and GMP to guanosine. The primary sequence of CNI is unrelated to CNII or ecto-5’nucleotisdase (e-N). The tissue distribution of cNI cNII is different, cNI is found only in vertebrate heart where as cNII is expressed in heart, brain and muscle. Sequence alignments of cytosolic 5’-nucleotidase, along with other nucleotidases, suggest that cytosolic 5’nucleotidase belong to a large superfamily of hydrolases with different substrate specificities and functional roles. Overproduction of cN-II could lead to resistance against anti-cancer drugs based on purine analogs. cN-II is Mg2+-dependent, regulated and stabilized by several factors such as allosteric effectors ATP and 2,3-DPG, although these are not directly involved in the reaction stoichiometry (2).
IMP and GMP and its derivatives specific cytosolic 5’-nucleotidase (cNII) acts through the formation of a phosphoenzyme intermediate. Phosphate either released leading to 5’-mononucleotide hydrolysis or transferred to an appropriate nucleoside acceptor, giving rise to a mononucleotide interconversion. Chemical reagents specifically modifying aspartate and glutamate residues inhibit the enzyme, and this inhibition is partially prevented by cN-II substrates and physiological inhibitors (3). Site-directed mutagenesis experiments confirmed the essential role of Asp-52 in the catalytic machinery of the enzyme and suggested also that Asp-54 assists in the formation of the acyl phosphate species. There exist a putative phsohorylation site on the cNII near C-termianl end of the protein.

General readings

Niu, W., et al. Clin. Chim. Acta 411 (19-20), 1491-1495 (2010)
Yamauchi, T., et al. Biochem. Pharmacol. 77(12):1780-1786 (2009)
Newton-Cheh, C., et al. Nat. Genet. (2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90 (2009)
Jordheim, L.P., et al. Drug Metab. Dispos. 36(12):2419-2423 (2008)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NT5C2 antibody

  Rabbit Polyclonal antibody to NT5C2 (5'-nucleotidase, cytosolic II)  
Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308031 diluted at 1:500. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NT5C2 (N-term) antibody

Western blot analysis in WiDr cell line lysates (35ug/lane) using NT5C2 antibody Cat.-No. AP52948PU-N. This demonstrates the NT5C2 antibody detected the NT5C2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

NT5C2 - human gene knockout kit via CRISPR

NT5C2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

NT5C2 - human gene knockout kit via CRISPR

NT5C2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

NT5C2 - human gene knockout kit via CRISPR

NT5C2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

NT5C2 - human gene knockout kit via CRISPR

NT5C2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nt5c2 - mouse gene knockout kit via CRISPR

Nt5c2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Nt5c2 - mouse gene knockout kit via CRISPR

Nt5c2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nt5c2 - mouse gene knockout kit via CRISPR

Nt5c2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Nt5c2 - mouse gene knockout kit via CRISPR

Nt5c2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NT5C2 (transcript variant 1)

  Recombinant protein of human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1  
NT5C2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NT5C2 (transcript variant 2)

  Recombinant protein of human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 2  
NT5C2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NT5C2 (1-561, His-tag)

NT5C2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

NT5C2 (1-561, His-tag)

NT5C2 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NT5C2 (mGFP-tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, NT5C2 (mGFP-tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, NT5C2 (mGFP-tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, NT5C2 (mGFP-tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, NT5C2 (Myc-DDK tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, NT5C2 (Myc-DDK tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, NT5C2 (Myc-DDK tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, NT5C2 (Myc-DDK tagged) - Human 5'-nucleotidase, cytosolic II (NT5C2), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Nt5c2 (GFP-tagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Nt5c2 (GFP-tagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,230.00
  OriGene Technologies, Inc.

Lenti ORF particles, Nt5c2 (GFP-tagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, Nt5c2 (GFP-tagged) - Mouse 5'-nucleotidase, cytosolic II (Nt5c2), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,190.00
  OriGene Technologies, Inc.

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