NTF97 antibody

Principal name

NTF97 antibody

Alternative names for NTF97 antibody

KPNB1, Nuclear factor P97, Importin-90, Importin subunit beta-1

SwissProt ID

P52296 (Rat), P70168 (Mouse), Q06142 (Yeast), Q14974 (Human)

Gene ID

3837 (KPNB1)

Available reactivities

Can (Canine), Hu (Human), Rt (Rat), Ms (Mouse), Por (Porcine), Bov (Bovine)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ChIP assay (ChIP), Neutralisation (NEUT), Flow Cytometry (F), Frozen Sections (C)

Background of NTF97 antibody

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq]

General readings

This product has been used in:Chi NC et al. Sequence and characterization of cytoplasmic nuclear protein import factor p97. J Cell Biol 130:265-74 (1995). PubMed PMID: 7615630
General / background references:Gorlich D & Mattaj IW Nucleocytoplasmic transport. Science 271:1513-8 (1996). PubMed PMID: 8599106
Powers MA & Forbes DJ Cytosolic factors in nuclear transport: what's importin? Cell 79:931-4 (1994). PubMed PMID: 8001141

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NTF97 antibody

TA303073 (0.03µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NTF97 antibody

Western blot analysis of Importin β expression in Jurkat (1), HeLa (2), Raji (3), HL-60 (4), A431 (5), L5178Y (6), WR19L (7), NIH/3T3 (8), CHO (9), PC12 (10) and Rat1 (11) using AM26500AF-N. Rat IgG2a 1D7 Azide Free Hu, Ms, Rt WB
0.1 mg / €360.00
  Acris Antibodies GmbH

NTF97 (851-863) antibody

Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hu, L, Mky, Ms, Op, Por, Prim, Rb, Rt, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

NTF97 (N-term) antibody

Western blot analysis of KPNB1 Antibody (N-term) (AP17524PU-N) in Y79 cell line lysates (35ug/lane). KPNB1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NTF97 antibody

KPNB1 polyclonal antibody ( Cat # PAB6909 ) ( 0.03 µg/ml ) staining of Daudi cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NTF97 (N-term) antibody

mouse, rat brain extract; WB Suggested Anti-KPNB1 Antibody . . Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience. Secondry Antibody Dilution : 1:20,000. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science; KPNB1 antibody - N-terminal region (ARP61178_P050) in mouse, rat brain extract cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

NTF97 antibody

Western Blot: KPNB1 Antibody [NB100-40784] - NB100-40784 (0.03ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

NTF97 antibody

Detection of KapB1 by Western Blot and  Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg)  from HeLa, 293T and mouse 3T3 cells. B. Whole cell lysate (1  mg for IP, 1/4 of IP loaded/lane) from HeLa cells. Antibodies:  Affinity purified rabbit anti-KapB1 antibody BL1962 (Cat. No.  NB100-79805) used at 1 mcg/ml for WB (A and B) and at 3  mcg/mg lysate for IP (B). Immunoprecipitation of KapB1 was  successful when using rabbit anti-KapB1 antibody BL1964 (Cat.  No. NB100-79806) at 3 mcg/mg lysate. Detection:  Chemiluminescence with exposure times of 30 seconds (A) and  10 seconds (B). Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NTF97 antibody

Detection of KapB1 by Western Blot and  Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg)  from HeLa, 293T and mouse 3T3 cells. B. Whole cell lysate (1  mg for IP, 1/4 of IP loaded/lane) from HeLa cells. Antibodies:  Affinity purified rabbit anti-KapB1 antibody BL1964 (Cat. No.  NB100-79806) used at 0.1 mcg/ml for WB (A and B) and at 3  mcg/mg lysate for IP (B). Immunoprecipitation of KapB1 was  unsuccessful when using rabbit anti-KapB1 antibody BL1962  (Cat. No. NB100-79805) at 3 mcg/mg lysate. Detection:  Chemiluminescence with an exposure time of 30 seconds (A and  B).  25043 West Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NTF97 antibody

Western Blot and Immunoprecipitation: KPNB1 Antibody [NB100-94993] - Detection of Human and Mouse KPNB1 on HeLa whole cell lysate using NB100-94993. Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

NTF97 antibody

Immunofluorescence: Importin beta Antibody (3E9) [NB120-2811] - Immunofluorescence with anti-Importin beta-1 Monoclonal Antibody [3E9]. Mouse IgG2a 3E9 Ascites Can, Hu, Ms, Por, Rt ChIP, ICC/IF, IP, NEUT, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

NTF97 antibody

Immunohistochemistry-Paraffin: Importin beta Antibody [NBP1-87712] - Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NTF97 (full length, C-term DDK tag)

NTF97 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

NTF97 (1-876, His-tag)

NTF97 Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

NTF97 (1-876, His-tag)

NTF97 Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

NTF97

NTF97 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NTF97

NTF97 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

17 Item(s)

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