ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)
Background of Nuclear protein NP60 / N-PAC antibody
N-PAC may have oxidoreductase activity. N-PAC regulates p38 MAP kise activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 sigling. N-PAC indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6
Immunofluorescence of purified MaxPab antibody to N-PAC on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot: N-PAC Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 61, 59 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.