Nucleolin antibody

Principal name

Nucleolin antibody

Alternative names for Nucleolin antibody

NCL

SwissProt ID

P19338 (Human)

Gene ID

4691 (NCL)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Pl (Plant), Bov (Bovine), African clawed frog, Can (Canine), Chk (Chicken), Insect, Mky (Monkey), Eq (Equine), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunoelectron Microscopy (EM), Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Nucleolin antibody

Nucleolin, which is identical to human DNA helicase IV, is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing. This 100 kDa protein has three major domains: a N-terminal domain comprised of long acidic stretches interspersed with basic repeats, similar to the structure of a high mobility group-type protein (this domain is responsible for the ablility of nucleolin to modulate chromatin condensation), a central domain that contains four RNA binding elements, a C-terminal domain approximately 85 amino acids long that is rich in glycine, arginine, and phenylalanine residues. Nucleolin fluctuates in parallel to DNA synthesis; intact 100 kDa protein is the major species in actively dividing cells, whereas the degraded forms are relativley abundant in nondividing cells. Nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Nucleolin also interacts directly with DNA topoisomerase I. It is located mainly in dense fibrillar regions of the nucleolus. Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. It interacts with APTX and contains 4 RNA recognition motif (RRM) domains.

General readings

Huang,Y., (2006) Blood 107 (9), 3564-3571

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nucleolin (Center) antibody

NCL Antibody (Center E443) (Cat. #TA324425) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow). Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

WB Suggested Anti-NCL Antibody Titration: 0.2-1 ug/mlPositive Control: JurkatNCL is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

Sample Type: 1. Human NT-2 cells (60ug); Primary Antibody Dilution: �2 ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: �1: 20, 000 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

Human Skin; NCL antibody - N-terminal region (ARP40583_P050) in Human Skin cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Nucleolin antibody

Human Kidney; Rabbit Anti-NCL Antibody. Catalog Number: ARP40584. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; NCL antibody - C-terminal region (ARP40584_T100) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

Nucleolin antibody

Western Blot:  Nucleolin Antibody -  Detection of nucleolin in crude PD31 nuclear extracts. Rabbit Purified Hu, Ms C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

Western Blot: Nucleolin Antibody - Western blot analysis of Nucleolin in HeLa nuclear extracts using anti-Nucleolin. Rabbit Purified Hu, Rt C, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

NCL Antibody (Center P291) (Cat. #TA324557) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow). Rabbit IgG Purified Hu, Mky ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Nucleolin pThr76/Thr84 antibody

Hela cell nuclear extract (2 x 106 cells) was phosphorylated in vitro using cdc2 kinase and ATP for 10 min. at 30C. Lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-nucleolin (Clone 10C7) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Lane 1 (-), no cdc2-kinase/ATP addition; Lane 2 (+), cdc2 kinase/ATP added. Mouse IgM 10C7 Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

Immunohistochemical detection of Nucleolin on paraffin embedded section of human stomach with AM26580AF-N. Mouse IgG1 4E2 Azide Free Hu F, ICC/IF, P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Nucleolin pThr76/Thr84 antibody

Hela cell nuclear extract (2 x 106 cells) was phosphorylated in vitro using cdc2 kinase and ATP for 10 min. at 30C. Lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-nucleolin (Clone 10C7) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Lane 1 (-), no cdc2-kinase/ATP addition; Lane 2 (+), cdc2 kinase/ATP added. Mouse IgM 10C7 Aff - Purified Hu P, WB
25 µg / €260.00
  Acris Antibodies GmbH

Nucleolin antibody

Nucleolin Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Nucleolin antibody

Western Blot analysis of NCL expression in transfected 293T cell line (H00004691-T02) by NCL MaxPab polyclonal antibody.

Lane 1: NCL transfected lysate(76.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Nucleolin antibody

In-situ immunofluorescent staining of NCL in PD31 murine pre-B cells using NCL polyclonal antibody ( Cat # PAB12376 ). Rabbit Hu, Ms C, ICC/IF, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

Nucleolin antibody

Nucleolin Rabbit Hu, Rt ICC/IF, IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

Nucleolin antibody

Nucleolin Rabbit Serum Hu, Rt ICC/IF, IP, P, WB
25 µl / €160.00
  Novus Biologicals Inc.

Nucleolin antibody

Western Blot and IP: Nucleolin Antibody [NB100-2237] - Detection of Human Nucleolin on HeLa whole cell lysate using NB100-2237. Nucleolin was also  immunoprecipitated using rabbit anti-Nucleolin antibodies NB100-2238 and NB100-2239. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Nucleolin antibody

Nucleolin Rabbit Serum Hu, Ms, Pl EM, ICC/IF, P, WB
25 µl / €200.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Nucleolin

Nucleolin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Nucleolin

Nucleolin Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

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