Nucleolin antibody

Principal name

Nucleolin antibody

Alternative names for Nucleolin antibody

NCL

SwissProt ID

P19338 (Human)

Gene ID

4691 (NCL)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Can (Canine), Chk (Chicken), Mky (Monkey), Eq (Equine), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Nucleolin antibody

Nucleolin (identical to human DNA helicase IV), is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing. Nucleolin is a 100 kDa protein which has three major domains: a N-terminal domain comprised of long acidic stretches interspersed with basic repeats, similar to the structure of a high mobility group-type protein (this domain is responsible for the ablility of nucleolin to modulate chromatin condensation), a central domain that contains four RNA binding elements and a C-terminal domain approximately 85 amino acids long that is rich in glycine, arginine, and phenylalanine residues. Nucleolin fluctuates in parallel to DNA synthesis; intact 100 kDa protein is the major species in actively dividing cells, whereas the degraded forms are relativley abundant in nondividing cells. Nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Nucleolin is primarily located in dense fibrillar regions of the nucleolus and is the major nucleolar protein of growing eukaryotic cells.
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

General readings

Huang,Y., (2006) Blood 107 (9), 3564-3571

  1. Quick-Filters:
  2. Highly Validated (3)

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nucleolin (Center) antibody

  Rabbit polyclonal NCL Antibody (Center E443)  
NCL Antibody (Center E443) (Cat. #TA324425) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow). Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

  Rabbit Polyclonal Anti-NCL Antibody  
WB Suggested Anti-NCL Antibody Titration: 0.2-1 ug/mlPositive Control: JurkatNCL is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

  Rabbit Polyclonal Anti-NCL Antibody  
Sample Type: 1. Human NT-2 cells (60ug); Primary Antibody Dilution: �2 ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: �1: 20, 000 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

  Rabbit Polyclonal Nucleolin Antibody  
Western Blot:  Nucleolin Antibody -  Detection of nucleolin in crude PD31 nuclear extracts. Rabbit Purified Hu, Ms C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

  Rabbit Polyclonal Nucleolin Antibody  
Western Blot: Nucleolin Antibody - Western blot analysis of Nucleolin in HeLa nuclear extracts using anti-Nucleolin. Rabbit Purified Hu, Rt C, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

  Rabbit polyclonal NCL Antibody (Center P291)  
NCL Antibody (Center P291) (Cat. #TA324557) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow). Rabbit IgG Purified Hu, Mky ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Nucleolin pThr76/Thr84 antibody

  Mouse Monoclonal anti-Nucleolin-Phospho (Thr76/Thr84) Antibody  
Hela cell nuclear extract (2 x 106 cells) was phosphorylated in vitro using cdc2 kinase and ATP for 10 min. at 30C. Lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-nucleolin (Clone 10C7) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Lane 1 (-), no cdc2-kinase/ATP addition; Lane 2 (+), cdc2 kinase/ATP added. Mouse IgM 10C7 Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nucleolin antibody

Immunohistochemical detection of Nucleolin on paraffin embedded section of human stomach with AM26580AF-N. Mouse IgG1 4E2 Azide Free Hu F, ICC/IF, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Nucleolin

  Recombinant protein of human nucleolin (NCL)  
Nucleolin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, NCL (mGFP-tagged)-Human nucleolin (NCL), 200 uL, >10^7 TU/mL

Lenti ORF particles, NCL (mGFP-tagged)-Human nucleolin (NCL), 200 uL, >10^7 TU/mL  
200 µl / €1,580.00
  OriGene Technologies, Inc.

Lenti ORF particles, NCL (Myc-DDK-tagged)-Human nucleolin (NCL), 200 uL, >10^7 TU/mL

Lenti ORF particles, NCL (Myc-DDK-tagged)-Human nucleolin (NCL), 200 uL, >10^7 TU/mL  
200 µl / €1,420.00
  OriGene Technologies, Inc.

11 Item(s)

per page
  • LinkedIn