Nucleostemin antibody

Principal name

Nucleostemin antibody

Alternative names for Nucleostemin antibody

GNL3, E2IG3, NS, Guanine nucleotide-binding protein-like 3, Nucleolar GTP-binding protein 3, Novel nucleolar protein 47, E2-induced gene 3 protein

SwissProt ID

Q7ZX41 (Xenla), Q811S9 (Rat), Q8CI11 (Mouse), Q9BVP2 (Human)

Gene ID

26354 (GNL3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Chicken, Goat, Mouse

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Nucleostemin antibody

This protein may play a role in maintaining stem cell self-renewal. Depletion or overexpression of nucleostemin reduces cell proliferation in CNS stem cells and transformed cells. Mutation analysis indicated that excessive nucleostemin, particularly mutants lacking the GTP regulatory domain, prevents cells from entering mitosis and causes apoptosis in a p53 dependent manner. The N terminal basic domain mediates the interaction with p53 and is required for the cell death caused by overexpression.

General readings

General / background references:Tsai RY & McKay RD A nucleolar mechanism controlling cell proliferation in stem cells and cancer cells. Genes Dev 16:2991-3003 (2002). PubMed PMID: 12464630

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nucleostemin antibody

Immunofluorescence of purified MaxPab antibody to GNL3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Nucleostemin (115-126) antibody

TA334188 (0.03µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nucleostemin antibody

WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate. GNL3 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Nucleostemin antibody

WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Nucleostemin antibody

TA348931 (2 ug/ml) staining of HeLa (A) HepG2 (B) and K562 (C) nuclear lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nucleostemin (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody (C-term) (cat.#AP11354PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

Nucleostemin antibody

Western blot analysis of anti-GNL3 Antibody (Center) (Cat#AP11355PU-N) in HL60 cell line lysates (35ug/lane). GNL3 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Nucleostemin antibody

Nucleostemin Mouse IgG2a 1A1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Nucleostemin antibody

Nucleostemin Mouse IgG2a 3A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Nucleostemin antibody

Nucleostemin Mouse IgG2a 1G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Nucleostemin antibody

Nucleostemin Mouse IgG2a 4B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Nucleostemin antibody

Nucleostemin Mouse IgG2a 2B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Nucleostemin antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 polyclonal antibody ( Cat # PAB2481 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

Nucleostemin antibody

Western blot analysis of GNL3 polyclonal antibody ( Cat # PAB2482 ) in HL-60 cell line lysate ( 35 µg/lane ) . GNL3 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Nucleostemin antibody

Human Stomach; WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; GNL3 antibody - N-terminal region (ARP58826_P050) in Human Stomach cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Nucleostemin antibody

Human 721_B; WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. GNL3 is supported by BioGPS gene expression data to be expressed in 721_B.; GNL3 antibody - middle region (ARP58827_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Nucleostemin antibody

Nucleostemin Goat Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €450.00
  Neuromics Antibodies

Nucleostemin antibody

Western Blot and IP: GNL3 Antibody [NB100-1568] - Detection of Human and Mouse GNL3 on HeLa whole cell lysate using NB100-1568. GNL3 was also immunoprecipitated using rabbit anti-GNL3 antibodies NB100-1569 and another GNL3 Ab. Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Nucleostemin antibody

Western Blot and IP: GNL3 Antibody [NB100-1569] - Detection of Human and Mouse GNL3 on HeLa whole cell lysate using NB100-1569. GNL3 was also immunoprecipitated using rabbit anti-GNL3 antibodies NB100-1568 and another GNL3 Ab. Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Nucleostemin antibody

Western Blot: E coli-derived fusion protein as test antigen. Affi-anti- RGS6 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-NS ) Chicken Aff - Purified Hu, Ms, Rt WB
50 µg / €520.00
  Novus Biologicals Inc.

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