Nucleostemin antibody

Principal name

Nucleostemin antibody

Alternative names for Nucleostemin antibody

GNL3, E2IG3, NS, Guanine nucleotide-binding protein-like 3, Nucleolar GTP-binding protein 3, Novel nucleolar protein 47, E2-induced gene 3 protein

SwissProt ID

Q7ZX41 (Xenla), Q811S9 (Rat), Q8CI11 (Mouse), Q9BVP2 (Human)

Gene ID

26354 (GNL3), 30877 (Gnl3), 290556

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of Nucleostemin antibody

Kit Component:
- KN307095G1, Gnl3 gRNA vector 1 in pCas-Guide vector
- KN307095G2, Gnl3 gRNA vector 2 in pCas-Guide vector
- KN307095D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Nucleostemin (115-126) antibody

  Goat Anti-GNL3 (aa115-126) Antibody  
TA334188 (0.03µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nucleostemin antibody

  Rabbit Polyclonal Anti-GNL3 Antibody - middle region  
WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate. GNL3 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Nucleostemin antibody

  Rabbit Polyclonal Anti-GNL3 Antibody - N-terminal region  
WB Suggested Anti-GNL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Nucleostemin antibody

  Goat Polyclonal Anti-GNL3 Antibody  
TA348931 (2 ug/ml) staining of HeLa (A) HepG2 (B) and K562 (C) nuclear lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Nucleostemin antibody

  Rabbit Polyclonal Anti-GNL3 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: K562 cells, Hela cells, LoVo cells, 293T cells, Raji cells, Primary antibody: (GNL3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Nucleostemin (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody (C-term) (cat.#AP11354PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

Nucleostemin antibody

Western blot analysis of anti-GNL3 Antibody (Center) (Cat#AP11355PU-N) in HL60 cell line lysates (35ug/lane). GNL3 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Nucleostemin antibody

Nucleostemin Goat Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €450.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

GNL3 - human gene knockout kit via CRISPR

GNL3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GNL3 - human gene knockout kit via CRISPR

GNL3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GNL3 - human gene knockout kit via CRISPR

GNL3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GNL3 - human gene knockout kit via CRISPR

GNL3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gnl3 - mouse gene knockout kit via CRISPR

Gnl3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Gnl3 - mouse gene knockout kit via CRISPR

Gnl3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gnl3 - mouse gene knockout kit via CRISPR

Gnl3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gnl3 - mouse gene knockout kit via CRISPR

Gnl3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Nucleostemin (full length, N-term HIS tag, transcript variant 1)

  Purified recombinant protein of Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
Nucleostemin Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

GNL3 overexpression lysate

GNL3 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

GNL3 overexpression lysate

GNL3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

GNL3 overexpression lysate

GNL3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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