NUMB antibody

Principal name

NUMB antibody

Alternative names for NUMB antibody

Protein S171, h-Numb, Protein numb homolog

SwissProt ID

P49757 (Human), Q9QZS3 (Mouse), Q9UKE9 (Human)

Gene ID

8650 (NUMB), 18222 (Numb)

Available reactivities

Hu (Human), Ms (Mouse), Chk (Chicken), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of NUMB antibody

NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells (1,2). NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.

General readings

1. Sczaniecka M et al. J Biol Chem. 2012 Apr 20;287(17):14052-68. PMID: 22337874
(WB on Human breast cancer cells).
2. Euskirchen P et al. Exp Cell Res. 2011 Dec 10;317(20):2864-73. PMID: 21939656
(in WB on Human malignant gliomas cells (U87).
3. Wu M et al. Dev Cell. 2010 Jul 20;19(1):114-25. PMID: 20643355
(in WB; IHC on Mouse primary epicardial cells).
4. Ito T et al. Nature. Nature. 2010 Aug 5;466(7307):765-8. PMID: 20639863
(in WB; IF on Mouse chronic myelogenous leukemia cells (CML).
5. Oliaro J et al. J Immunol. 2010 Jul 1;185(1):367-75. PMID: 20530266
(in IF on Mouse Bone marrow cells).
6. Kharas MG et al. Nat Med. 2010 Aug;16(8):903-8. PMID: 20616797
7. Ma Q et al. Cell Signal. 2008 Jun;20(6):1044-51. PMID: 18299187
8. Corallini S et al. Reproduction. 2006 Dec;132(6):887-97. PMID: 17127749
9. Di Marcotullio L et al. Nat Cell Biol. 2006 Dec;8(12):1415-23. PMID: 17115028
10. Holowacz T et al. Dev Dyn. 2006 Mar;235(3):633-45. PMID: 16425215
11. Zigman M et al. Neuron. 2005 Nov 23;48(4):539-45. PMID: 16301171
12. Fassa A et al. J Neurosci Res. 2005 Oct 15;82(2):214-24. PMID: 16175584
13. Sanada K et al. Cell. 2005 Jul 15;122(1):119-31. PMID: 16009138

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NUMB control peptide

NUMB
50 µg / €160.00
  Acris Antibodies GmbH

NUMB antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA501561) Mouse IgG1 OTI5A9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

Anti-NUMB mouse monoclonal antibody (TA501614) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUMB(RC216230 ). Mouse IgG1 OTI1A7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168) Mouse IgG2a OTI4F3 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802138) Mouse IgG2a OTI1B5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802143) Mouse IgG1 OTI2E8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802163) Mouse IgG1 OTI2F1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB (C-term) antibody

Antibody staining (0.3µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, ICC/IF, IP, WB
20 µg / €200.00
  Acris Antibodies GmbH

NUMB (C-term) antibody

Antibody staining (0.3µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NUMB (C-term) antibody

Western blot analysis of NUMB in mouse lung tissue lysate with NUMB antibody at (A) 0.25 and (B) 0.5 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NUMB antibody

Western Blot: NUMB Antibody [TA336652] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of TA336652. ECL detection: 30 seconds. Rabbit Purified Chk, Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NUMB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG1 OTI2C4 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG2a OTI6B2 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG2a OTI2G1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802132) Mouse IgG1 OTI2B9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG2a OTI1E3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG2a OTI4C6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG1 OTI4D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG1 OTI1A11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802162) Mouse IgG2a OTI3H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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