NUMB antibody

Principal name

NUMB antibody

Alternative names for NUMB antibody

Protein S171, h-Numb, Protein numb homolog

SwissProt ID

P49757 (Human), Q9QZS3 (Mouse), Q9UKE9 (Human)

Gene ID

8650 (NUMB), 18222 (Numb)

Available reactivities

Hu (Human), Ms (Mouse), Chk (Chicken), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NUMB antibody

Kit Component:
- KN311341G1, Numb gRNA vector 1 in pCas-Guide vector
- KN311341G2, Numb gRNA vector 2 in pCas-Guide vector
- KN311341D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Sczaniecka M et al. J Biol Chem. 2012 Apr 20;287(17):14052-68. PMID: 22337874.
2. Euskirchen P et al. Exp Cell Res. 2011 Dec 10;317(20):2864-73. PMID: 21939656.
3. Wu M et al. Dev Cell. 2010 Jul 20;19(1):114-25. PMID: 20643355.
4. Ito T et al. Nature. Nature. 2010 Aug 5;466(7307):765-8. PMID: 20639863.
5. Oliaro J et al. J Immunol. 2010 Jul 1;185(1):367-75. PMID: 20530266. 
6. Kharas MG et al. Nat Med. 2010 Aug;16(8):903-8. PMID: 20616797.
7. Ma Q et al. Cell Signal. 2008 Jun;20(6):1044-51. PMID: 18299187.
8. Corallini S et al. Reproduction. 2006 Dec;132(6):887-97. PMID: 17127749.
9. Di Marcotullio L et al. Nat Cell Biol. 2006 Dec;8(12):1415-23. PMID: 17115028.
10. Holowacz T et al. Dev Dyn. 2006 Mar;235(3):633-45. PMID: 16425215.
11. Zigman M et al. Neuron. 2005 Nov 23;48(4):539-45. PMID: 16301171.
12. Fassa A et al. J Neurosci Res. 2005 Oct 15;82(2):214-24. PMID: 16175584.
13. Sanada K et al. Cell. 2005 Jul 15;122(1):119-31. PMID: 16009138.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI5A9 (formerly 5A9)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA501561) Mouse IgG1 OTI5A9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)  
Anti-NUMB mouse monoclonal antibody (TA501614) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUMB(RC216230 ). Mouse IgG1 OTI1A7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI4F3 (formerly 4F3)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502168) Mouse IgG2a OTI4F3 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)  
Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802138) Mouse IgG2a OTI1B5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI2E8 (formerly 2E8)  
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802143) Mouse IgG1 OTI2E8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI2F1 (formerly 2F1)  
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802163) Mouse IgG1 OTI2F1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB (C-term) antibody

  Goat Polyclonal Antibody against NUMB  
Antibody staining (0.3µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NUMB (C-term) antibody

  Rabbit Polyclonal NUMB Antibody  
Western blot analysis of NUMB in mouse lung tissue lysate with NUMB antibody at (A) 0.25 and (B) 0.5 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NUMB antibody

  Rabbit Polyclonal Numb Antibody  
Western Blot: NUMB Antibody [TA336652] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of TA336652. ECL detection: 30 seconds. Rabbit Purified Chk, Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NUMB antibody

  Rabbit Polyclonal Anti-NUMB Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-6: K562 cells, hela cells, skov3 cells, NIH/3T3 cells, Raji cells, human testis tissue, Primary antibody: (NUMB Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI2C4 (formerly 2C4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG1 OTI2C4 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI6B2 (formerly 6B2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG2a OTI6B2 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG2a OTI2G1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)  
Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802132) Mouse IgG1 OTI2B9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG2a OTI1E3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI4C6 (formerly 4C6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG2a OTI4C6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI4D3 (formerly 4D3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG1 OTI4D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI1A11 (formerly 1A11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG1 OTI1A11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI3H8 (formerly 3H8)  
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802162) Mouse IgG2a OTI3H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

NUMB antibody

  NUMB mouse monoclonal antibody, clone OTI3B2 (formerly 3B2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Mouse IgG1 OTI3B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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