NUP50 antibody

Principal name

NUP50 antibody

Alternative names for NUP50 antibody

Nucleoporin 50 kDa, NPAP60L, Nuclear pore-associated protein 60 kDa-like

SwissProt ID

O08587 (Rat), Q9JIH2 (Mouse), Q9UKX7 (Human)

Gene ID

10762 (NUP50), 25497 (Npap60)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Goat, Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of NUP50 antibody

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Ogawa, Y., et al. Mol. Biol. Cell 21(4):630-638(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NUP50 antibody

Host: Rabbit; Target Name: NUP50; Sample Tissue: HepG2; Antibody Dilution: 1.0 ug/mlThere is BioGPS gene expression data showing that NUP50 is expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

NUP50 antibody

Human HeLa; . Sample Type : Hela cell lysate .; NUP50 antibody - C-terminal region (ARP52288_P050) in Human HeLa cells using Immunofluorescence Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

NUP50 (C-term) antibody

TA302862 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

NUP50 antibody

Host: Rabbit; Target Name: Npap60; Sample Tissue: Rat Brain lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

NUP50 (C-term) antibody

Western blot analysis of NUP50 (arrow) using rabbit polyclonal NUP50 Antibody (C-term) (AP11702PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NUP50 (Center) antibody

Western blot analysis of NUP50 Antibody (Center) Cat.-No AP52974PU-N in WiDr cell line lysates (35ug/lane). This demonstrates the NUP50 antibody detected the NUP50 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

NUP50 antibody

Immunofluorescence of purified MaxPab antibody to NUP50 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

NUP50 antibody

Immunofluorescence of purified MaxPab antibody to NUP50 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

NUP50 antibody

NUP50 Mouse IgG2b 4H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

NUP50 antibody

Western blot analysis of NUP50 ( arrow ) usingNUP50 polyclonal antibody ( Cat # PAB3709 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the NUP50 gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

NUP50 antibody

NUP50 polyclonal antibody ( Cat # PAB6369 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

NUP50 antibody

Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-1212] - 3.8ug/ml staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

NUP50 antibody

Western Blot and Immunoprecipitation: NUP50 Antibody [NB100-93324] - Detection of Human NUP50 on HeLa whole cell lysate using NB100-93324. NUP50 was also  immunoprecipitated by rabbit anti-NUP50 antibody NB100-93323. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

NUP50 (transcript variant 3)

NUP50 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NUP50 (transcript variant 2)

NUP50 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

NUP50

NUP50 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NUP50

NUP50 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

NUP50

NUP50 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

NUP50

NUP50 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NUP50 293T Cell Transient Overexpression Lysate(Denatured)

NUP50 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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