Occludin antibody

Principal name

Occludin antibody

Alternative names for Occludin antibody

OCLN

SwissProt ID

Q16625 (Human), Q28269 (Canfa), Q61146 (Mouse), Q6P6T5 (Rat), Q91049 (Chick), Q9PUN1 (Xenla)

Gene ID

4950 (OCLN), 100506658, 87 (ACTN1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Por (Porcine)

Available hosts

Mouse, Rabbit, Guinea Pig

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of Occludin antibody

Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

General readings

Shen,L., (2008) J. Cell Biol. 181 (4), 683-695

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Occludin antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between OCLN and CSNK1E. HeLa cells were stained with anti-OCLN rabbit purified polyclonal 1:1200 and anti-CSNK1E mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Occludin (C-term) antibody

Human Lung stained with Anti-Occludin antibody Cat.-No AP15690PU Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Occludin antibody

Human Lung stained with Anti-Occludin antibody Cat.-No AP15690PU Rabbit Purified Hu P, WB
1 ml / €520.00
  Acris Antibodies GmbH

Occludin (C-term) antibody

Human Lung stained with Anti-Occludin antibody Cat.-No AP15690PU Rabbit Purified Hu P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

Occludin antibody

Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Occludin antibody

Predicted band size: 59 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Occludin antibody

Predicted cell location: Cytoplasm . Positive control: Human colon and lung cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using OCLN antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Occludin (358-504) antibody

Occludin Guinea Pig Ig Purified Can, Hu, Ms, Rt C, ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Occludin Control Peptide

Occludin
50 µg / €160.00
  Acris Antibodies GmbH

Occludin antibody

OCLN polyclonal antibody (A01), Lot # 051207JC01 Western Blot analysis of OCLN expression in Jurkat ( Cat # L017V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Occludin antibody

Western Blot analysis of OCLN expression in transfected 293T cell line (H00004950-T01) by OCLN MaxPab polyclonal antibody.

Lane 1: OCLN transfected lysate(57.42 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Occludin antibody

Occludin Rabbit Hu, Ms IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Occludin antibody

Immunofluorescence of monoclonal antibody to OCLN on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 1G7 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Occludin antibody

Occludin Mouse IgG1 5A7 Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Occludin antibody

Occludin Mouse IgM 2B3 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

Occludin antibody

Immunohistochemical staining of OCLN on formalin fixed, paraffin embedded human lung with OCLN polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Occludin antibody

Transfected 293T; WB Suggested Anti-OCLN Antibody Titration: 2.5ug/ml. Positive Control: Transfected 293T; OCLN antibody - N-terminal region (ARP42888_T100) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

Occludin antibody

Human COLO205; WB Suggested Anti-OCLN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate; OCLN antibody - N-terminal region (ARP42889_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Occludin antibody

Western Blot: occludin Antibody [NBP1-59358] - This Anti-OCLN antibody was used in Western Blot of Transfected 293T tissue lysate at a concentration of 2.5ug/ml. Rabbit Purified Can, Hu, Ms, Rt WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Occludin antibody

Western Blot: occludin Antibody [NBP1-59435] - This Anti-OCLN antibody was used in Western Blot of COLO205     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Xen, Ze WB
50 µg / €440.00
  Novus Biologicals Inc.

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