OCT3/4 antibody

Principal name

OCT3/4 antibody

Alternative names for OCT3/4 antibody

POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding transcription factor 3, Oct-3, Oct-4

SwissProt ID

O97552 (Bovin), P20263 (Mouse), Q01860 (Human), Q9TSV5 (Pig)

Gene ID

5460 (POU5F1), 4747 (NEFL), 18999 (Pou5f1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Gt (Goat), Can (Canine), Bov (Bovine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Fe (Feline), Chimp (Chimpanzee), Macaque, Mink, Prim (Primate), Eq (Equine)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C), Dot blot (Dot)

Background of OCT3/4 antibody

Oct-4, also known as POU domain transcription factor, Octomer- binding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression; the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.

General readings

Jones TD, Ulbright TM, Eble JN, Baldridge LA, Cheng L. OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma. Am J Surg Pathol. 2004 Jul;28(7):935-40. PMID: 15223965

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

OCT3/4 antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035) Mouse IgG1 OTI9B7 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

OCT3/4 antibody

Anti-POU5F1 mouse monoclonal antibody (TA506798) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POU5F1(RC211998). Mouse IgG1 OTI7H1 Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

OCT3/4 (6-152) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu, Mky, Por, Rb F, ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP15694PU Oct3/Oct4 antibody staining of Human Seminoma. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP15694PU Oct3/Oct4 antibody staining of Human Seminoma. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP15694PU Oct3/Oct4 antibody staining of Human Seminoma. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP15695PU Oct3/Oct4 antibody staining of Human Seminoma. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP15695PU Oct3/Oct4 antibody staining of Human Seminoma. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP15695PU Oct3/Oct4 antibody staining of Human Seminoma. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

OCT3/4 antibody

AP22409PU-N OCT4 antibody staining of NIH3T3 lysate at 1 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

OCT3/4 antibody

Detection of OCT4 in mouse brain lysate Rabbit IgG Purified Hu, Mky WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

OCT3/4 antibody

Anti-POU5F1 antibody (2µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

OCT3/4 antibody

Western blot analysis of POU5F1 in mouse liver tissue lysate with POU5F1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

OCT3/4 antibody

WB using Anti-Oct4 antibody shows detection of endogenous Oct4 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kDa (arrow head) corresponds to Oct4. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1:1,000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with Oct4 (lanes 3 and 4, respectively). Rabbit IgG Aff - Purified Chimp, Eq, Hu, Macaque, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

OCT3/4 antibody

F9 mouse embryonal carcinoma cell line (left) and mouse splenocyte (right) lysates were loaded at 1x10e5 cells/lane, probed with 2 ug/mL of Anti-Mouse OCT3/4 Purified and revealed with Anti-Rat IgG HRP. Rat EM92 Purified Ms P, WB
25 µg / €150.00
  OriGene Technologies, Inc.

OCT3/4 antibody

Predicted band size: 39 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

OCT3/4 antibody

Western blot analysis of OCT3/4 Antibody (E125) in HL60 cell line lysates (35ug/lane). OCT3/4 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

OCT3/4 antibody

Western blot analysis of extracts from HeLa cells, using OCT3 antibody (#TA326116).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

OCT3/4 antibody

Immunohistochemistry staining of Paraffin Seminoma tissue by OCT-4 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear) Mouse IgG MRQ-10 Purified Hu P
1 ml / €490.00
  OriGene Technologies, Inc.

OCT3/4 antibody

Immunofluorescent microscope analysis of NTERA-2 cells using anti-Oct4 polyclonal antibody (poly6319) (red). α-tubulin (clone 10D8) has been labeled with green, and nuclei were stained with DAPI (blue). Rabbit IgG Poly6319 Purified Hu ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

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