OGT antibody

Principal name

OGT antibody

Alternative names for OGT antibody

O-GlcNAc transferase subunit p110, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-linked N-acetylglucosamine transferase 110 kDa subunit

SwissProt ID

O15294 (Human), O18158 (Caeel), P56558 (Rat), P81436 (Rabit), Q27HV0 (Pig), Q8CGY8 (Mouse)

Gene ID

8473 (OGT), 108155 (Ogt), 26295

Available reactivities

Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Fi (Fish), Hst (Hamster), Hu (Human), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), Bear, Dros (Drosophila), Ze (Zebrafish), African clawed frog, GP (Guinea Pig)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of OGT antibody

OGT catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization sigl. Two altertively spliced transcript variants encoding distinct isoforms have been found for this gene. O-linked N-acetylglucosamine (O-Glcc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization sigl. Two altertively spliced transcript variants encoding distinct isoforms have been found for this gene.

General readings

Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS.
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.
Diabetes. 2004 Dec;53(12):3184-92.
PMID: 15561949

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

OGT antibody

  Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase))  
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug A431 whole cell extract. C. 30 ug HeLa whole cell extract. D. 30 ug HepG2 whole cell extract. 7.5 % SDS-PAGE. O-GlcNAc Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

OGT antibody

  Rabbit Polyclonal antibody to O-GlcNAc transferase (O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase))  
Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. TA308773 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

OGT antibody

  OGT Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using OGT antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

OGT antibody

  Goat Polyclonal Antibody against OGT  
TA302556 (0.05µg/ml) staining of Rat Pancreas lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

OGT antibody

  Rabbit polyclonal Anti-OGT Antibody  
Host: Rabbit; Target Name: OGT; Sample Tissue: 721_B Whole Cell lysates; Antibody Dilution: 1.0ug/mlOGT is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

OGT (543-555) antibody

Antibody (0.05 µg/ml) staining of Rat Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

OGT (C-term) antibody

Western blot analysis of OGT antibody (C-term) (AP17608PU-N) in mouse bladder tissue lysates (35ug/lane). OGT (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

OGT - human gene knockout kit via CRISPR

OGT - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

OGT - human gene knockout kit via CRISPR

OGT - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

OGT - human gene knockout kit via CRISPR

OGT - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

OGT - human gene knockout kit via CRISPR

OGT - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ogt - mouse gene knockout kit via CRISPR

Ogt - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ogt - mouse gene knockout kit via CRISPR

Ogt - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ogt - mouse gene knockout kit via CRISPR

Ogt - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ogt - mouse gene knockout kit via CRISPR

Ogt - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

OGT (transcript variant 1)

  Recombinant protein of human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1  
OGT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

OGT (transcript variant 2)

  Recombinant protein of human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 2  
OGT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

OGT overexpression lysate

OGT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

OGT overexpression lysate

OGT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

OGT overexpression lysate

OGT overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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