OGT antibody

Principal name

OGT antibody

Alternative names for OGT antibody

O-GlcNAc transferase subunit p110, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-linked N-acetylglucosamine transferase 110 kDa subunit

SwissProt ID

O15294 (Human), O18158 (Caeel), P56558 (Rat), P81436 (Rabit), Q27HV0 (Pig), Q8CGY8 (Mouse)

Gene ID

8473 (OGT)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Dros (Drosophila), Por (Porcine), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of OGT antibody

Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related; the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.

General readings

Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS.
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.
Diabetes. 2004 Dec;53(12):3184-92.
PMID: 15561949

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

OGT antibody

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug A431 whole cell extract. C. 30 ug HeLa whole cell extract. D. 30 ug HepG2 whole cell extract. 7.5 % SDS-PAGE. O-GlcNAc Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

OGT antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. TA308773 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

OGT antibody

Western blot analysis of extracts of various cell lines, using OGT antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

OGT antibody

TA302556 (0.05µg/ml) staining of Rat Pancreas lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

OGT antibody

Host: Rabbit; Target Name: OGT; Sample Tissue: 721_B Whole Cell lysates; Antibody Dilution: 1.0ug/mlOGT is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

OGT (543-555) antibody

Antibody (0.05 µg/ml) staining of Rat Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

OGT (C-term) antibody

Western blot analysis of OGT antibody (C-term) (AP17608PU-N) in mouse bladder tissue lysates (35ug/lane). OGT (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

OGT antibody

OGT polyclonal antibody ( Cat # PAB7209 ) ( 0.05 µg/ml ) staining of rat pancreas lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

OGT antibody

Human Pineal Tissue; Rabbit Anti-OGT Antibody . Catalog Number: ARP49154_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in processes of pinealocytes and endothelial cells in blood vessels . Primary Antibody Concentration: 1:100 . Other Working Concentrations: 1/600 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-OGT Antibody (ARP49154_P050) ) in Human Pineal Tissue using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

OGT antibody

Western Blot: OGT - Golgi Complex Marker Antibody [NB100-57845] - NB100-57845 (0.05µg/ml) staining of Rat Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

OGT antibody

Immunohistochemistry-Paraffin: O-GlcNAc Antibody [NBP1-32791] - Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using O-GlcNAc transferase antibody at 1:100 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

OGT antibody

Western Blot: O-GLCNAC Antibody [NBP1-52925] - This Anti-OGT antibody was used in Western Blot of Fetal Heart tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

OGT antibody

Immunohistochemistry-Paraffin: O-GlcNAc Antibody [NBP1-89844] - Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

OGT antibody

Immunohistochemistry-Paraffin: O-GlcNAc Antibody [NBP1-89845] - Immunohistochemical staining of human liver shows moderate nuclear and cytoplasmic positivity in hepatocytes. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

OGT (transcript variant 1)

OGT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

OGT (transcript variant 2)

OGT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

OGT

OGT Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

OGT

OGT Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

OGT overexpression lysate

OGT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

OGT overexpression lysate

OGT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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