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OLIG2 antibody

Principal name

OLIG2 antibody

Alternative names for OLIG2 antibody

OLIG2, BHLHB1, PRKCBP2, RACK17, Oligodendrocyte transcription factor 2, Oligo2, bHLHB1, Class B basic helix-loop-helix protein 1, Protein kinase C-binding protein RACK17, Protein kinase C-binding protein 2

SwissProt ID

Q13516 (Human), Q90XB3 (Chick), Q9EQW6 (Mouse)

Gene ID

10215 (OLIG2), 50913 (Olig2)

Ncbi ID

NP_005797.1, NP_005797, 9606, NP_058663, NP_058663.2, NM_005806

Available reactivities

Hu (Human), Chk (Chicken), Ms (Mouse), Bov (Bovine), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Goat, Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of OLIG2 antibody

OLIG2 is a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. OLIG2 is an essential regulator of ventral neuroectodermal progenitor cell fate. It is associated with T-cell acute lymphoblastic leukemia due to a chromosomal translocation t(14;21)(q11.2;q22). OLIG2 might play a role in learning deficits associated with Down syndrome.This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Mitkus,S.N., Schizophr. Res. 98 (1-3), 129-138 (2008)

Human; . Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage Cells. Dilution:. 1:500; OLIG2 Antibody - C-terminal region (ARP32753_P050) in Human cells using Immunohistochemistry

ARP32753_P050

Fetal Lung; Host: Rabbit. Target Name:OLIG2. Sample Tissue:Fetal Lung. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane. Gel Concentration: 0.12%.

ARP32753_P050

Human Fetal Lung; OLIG2 Antibody - C-terminal region (ARP32753_P050) validated by WB using Fetal Lung cell lysate at 1.0ug/ml.; OLIG2 Antibody - C-terminal region (ARP32753_P050) in Human Fetal Lung cells using Western Blot

ARP32753_P050

Immunoperoxidase of monoclonal antibody to OLIG2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

H00010215-M03

Western Blot analysis of OLIG2 expression in transfected 293T cell line (H00010215-T01) by OLIG2 MaxPab polyclonal antibody.Lane 1: OLIG2 transfected lysate(35.53 KDa).Lane 2: Non-transfected lysate.

H00010215-B01

Western Blot analysis of OLIG2 expression in transfected 293T cell line (H00010215-T01) by OLIG2 MaxPab polyclonal antibody.Lane 1: OLIG2 transfected lysate(32.40 KDa).Lane 2: Non-transfected lysate.

H00010215-D01P

human LCL and mouse brains; . Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage Cells. Dilution:. 1:500; OLIG2 antibody - N-terminal region (ARP31464_P050) in human LCL and mouse brains cells using Immunohistochemistry

ARP31464_P050

human LCL and mouse brains; OLIG2 antibody - N-terminal region (ARP31464_P050) validated by WB using human LCL and mouse brains at 1:1000.; OLIG2 antibody - N-terminal region (ARP31464_P050) in human LCL and mouse brains cells using Western Blot

ARP31464_P050

Human 293T; WB Suggested Anti-OLIG2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; OLIG2 antibody - N-terminal region (ARP31464_P050) in Human 293T cells using Western Blot

ARP31464_P050

Human optic nerve; Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage CellsDilution: 1:500; OLIG2 antibody - N-terminal region (ARP32752_P050) in Human optic nerve cells using Immunohistochemistry

ARP32752_P050

Human HepG2; WB Suggested Anti-OLIG2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; OLIG2 antibody - N-terminal region (ARP32752_P050) in Human HepG2 cells using Western Blot

ARP32752_P050

Immunoprecipitation of OLIG2 transfected lysate using anti-OLIG2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with OLIG2 MaxPab mouse polyclonal antibody (B01) (H00010215-B01).

H00010215-D01

Human Brain, Oligodendroglia: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM31746PU-N

Immunohistochemical analysis of paraffin-embedded muscle tissues using OLIG2 antibody Cat.-No AM06579SU-N with DAB staining.

AM06579SU-N

Immunofluorescence analysis of U251 cells using OLIG2 antibody Cat.-No AM06579SU-N (green). Blue: DRAQ5 fluorescent DNA dye.

AM06579SU-N

Western blot analysis using OLIG2 antibody Cat.-No AM06579SU-N against HEK293 (1) and OLIG2(AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.

AM06579SU-N

AM06579SU-N

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of glial cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

TA590593

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & golgi apparatus.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

TA590593

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OLIG2 (RC208209, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OLIG2.

TA590593

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

TA590593

TP308209

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 32k Olig2 protein.

TA309205

WB Suggested Anti-OLIG2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate

TA331141

Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage CellsDilution: 1:500

TA331141

OLIG2 Antibody - C-terminal region validated by WB using Fetal Lung cell lysate at 1.0ug/ml.

TA331142

Host: Rabbit; Target Name:OLIG2; Sample Tissue:Fetal Lung; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:1ug/ml; Peptide Concentration: 5ug/ml; Lysate Quantity: 25ug/lane; Gel Concentration: 0.12%

TA331142

Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage Cells; Dilution: 1:500

TA331142

Western blot detection of OLIG2 in CHO-K1 cell lysate(B)and CHO-K1 transfected by EGFP-OLIG2 fragment(A)cell lysate using OLIG2 mouse mAb (1:1000 diluted).