Oncostatin-M antibody

Principal name

Oncostatin-M antibody

Alternative names for Oncostatin-M antibody

OSM

SwissProt ID

P13725 (Human), P53346 (Bovin), P53347 (Mouse)

Gene ID

5008 (OSM), 18413 (Osm)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Neutralisation (NEUT), Paraffin Sections (P), Functional assay (FN), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Oncostatin-M antibody

Oncostatin M (OSM) is a member of the IL-6 family of cytokines, which includes IL-6, IL-11, LIF, ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1) and cardiotropin-like cytokine. Structurally, OSM is a 196 amino acid monomeric glycoprotein with two potential N-linked glycosylation sites and five cysteine residues, four of which form disulfide bonds.OSM is a growth regulator, in particular with respect to human fibroblasts and vascular smooth muscle cells. Additionally, OSM stimulates the production of IL-6, G-CSF and GM-CSF from endothelial cells and hence plays a role in hematopoiesis and myelopoiesis. OSM mediates the differentiation of megakaryocytes, as well as the regulation of cholesterol metabolism, bone formation and wound healing. OSM has also been shown to play a role in both pro and anti-inflammatory actions. The expression of OSM from activated T-cells and macrophages is implicated in the initiation phase of an inflammatory response. OSM induces inflammation and destruction of mouse joints in vivo and an elevated level of OSM is present in joints of patients with rheumatoid arthritis. OSM may also be involved in multiple sclerosis (MS), as it is present in MS lesions in microglial cells and infiltrating leukocytes. Conversely, mice with myelin antigen induced encephalomyelitis exhibit symptom reduction when treated with human OSM. The actions of OSM are exerted through binding to a heterodimeric membrane receptor composed of either the OSM receptor or LIF receptor subunit associated with the gp 130-receptor chain. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

General / background references:Grove RI et al. Oncostatin M up-regulates low density lipoprotein receptors in HepG2 cells by a novel mechanism. J Biol Chem 266:18194-9 (1991). PubMed PMID: 1655740
Brown TJ et al. Regulation of IL-6 expression by oncostatin M. J Immunol 147:2175-80 (1991). PubMed PMID: 1918953
Hamilton JA et al. Oncostatin M stimulates urokinase-type plasminogen activator activity in human synovial fibroblasts. Biochem Biophys Res Commun 180:652-9 (1991). PubMed PMID: 1953736
Brown TJ et al. Purification and characterization of cytostatic lymphokines produced by activated human T lymphocytes. Synergistic antiproliferative activity of transforming growth factor beta 1, interferon-gamma, and oncostatin M for human melanoma cells. J Immunol 139:2977-83 (1987). PubMed PMID: 3117884

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Oncostatin-M antibody

Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-OSMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500195, Dilution 1:50) Mouse IgG1 OTI2B6 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Oncostatin-M antibody

To detect hOncostatin M by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hOncostatin M. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Oncostatin-M antibody

To detect hOncostatin M by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Oncostatin M (TA328409) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOncostatin M. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Oncostatin-M antibody

Oncostatin-M Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Oncostatin-M antibody

Oncostatin-M Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Oncostatin-M antibody

Formalin-Fixed, Paraffin-Embedded sections of human breast malignant ductal adenocarcinoma stained with  Oncostatin-M Antibody Cat.-No PP1060P.  Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

Oncostatin-M antibody

Oncostatin-M Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Oncostatin-M antibody

Western Blot analysis of OSM expression in transfected 293T cell line (H00005008-T01) by OSM MaxPab polyclonal antibody.

Lane 1: OSM transfected lysate(27.83 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Oncostatin-M antibody

Western Blot analysis of OSM expression in transfected 293T cell line (H00005008-T02) by OSM MaxPab polyclonal antibody.

Lane 1: OSM transfected lysate(27.72 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Oncostatin-M antibody

Western Blot analysis of OSM expression in transfected 293T cell line (H00005008-T02) by OSM MaxPab polyclonal antibody.

Lane 1: OSM transfected lysate(27.72 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Oncostatin-M antibody

Western Blot analysis of OSM expression in transfected 293T cell line (H00005008-T02) by OSM MaxPab polyclonal antibody.

Lane 1: OSM transfected lysate(28.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Oncostatin-M antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between OSM and COL4A6. HeLa cells were stained with anti-OSM rabbit purified polyclonal 1:1200 and anti-COL4A6 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Oncostatin-M antibody

Oncostatin-M Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €290.00
  Boster Immunoleader

Oncostatin-M antibody

Oncostatin-M Mouse IgG2a 17001,31 Purified Hu E, NEUT, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

Oncostatin-M antibody

Immunohistochemistry-Paraffin: Oncostatin M Antibody (2B6) [NBP1-47904] - Immunohistochemical staining of paraffin-embedded lung tissue using anti-OSMmouse monoclonal antibody. (Dilution 1:50) Mouse 2B6 Aff - Purified Hu P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

Oncostatin-M antibody

Immunohistochemistry-Paraffin: Oncostatin M Antibody [NBP1-87768] - Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Oncostatin-M

Oncostatin-M Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Oncostatin-M

Oncostatin-M Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 10 µg / €199.00

Special Price: 10 µg / €139.00

  OriGene Technologies, Inc.

Oncostatin-M

Oncostatin-M Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 10 µg / €199.00

Special Price: 10 µg / €139.00

  OriGene Technologies, Inc.

Oncostatin-M

Oncostatin-M Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €399.00
  OriGene Technologies, Inc.

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