ORC2 antibody

Principal name

ORC2 antibody

Alternative names for ORC2 antibody

RRR1, SIR5, YBR060C, YBR0523, ORC2L, Origin recognition complex subunit 2, Origin recognition complex 71 kDa subunit

SwissProt ID

A6QNM3 (Bovin), P32833 (Yeast), Q13416 (Human), Q60862 (Mouse), Q75PQ8 (Rat), Q91628 (Xenla)

Gene ID

4999 (ORC2), 852352

Available reactivities

Hu (Human), Ye (Yeast), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ORC2 antibody

ORC, the origin recognition complex, is a multi-subunit protein complex that is composed of 6 different proteins ranging in molecular weight from 50 to 120 kDa. The complex was originally identified in S. cerevisiae and is essential for the initiation of DNA replication. The proteins bind to a specific recognition sequence in the origin region and remain bound to the DNA throughout the cell cycle. The second largest of the proteins, the 71 kDa Orc2p, is encoded by ORC2. ORC2, also called RRR1 or SIR5, is an essential gene in S. cerevisiae and is required for both replication and transcriptional silencing. ORC is present at little more than one complete complex per replication origin and binds at origins immediately after initiation in the previous cell cycle. Assembly of additional complexes occurs in multiple stages during the cell cycle and includes a pre-replicative complex (pre-RC) as well as other proteins. Orc2p, along with Orc5p, forms a complex with Cdc6p, a protein required for initiation of DNA replication. Since ORC and Cdc6p bind as a complex at individual DNA replication origins, ORC and Cdc6p may cooperate to determine the frequency of initiation of replication and also repress transcription of specific genes. Although the origin of DNA replication has been difficult to demonstrate in other eukaryotic species, the ORC function has been conserved through eukaryotes with orthologs of ORC2 having been identified in other eukaryotes including Humans, Mice, C. elegans, Drosophila, Xenopus, and various Yeast and plant species.

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (1)

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ORC2 antibody

Immunoperoxidase of monoclonal antibody to ORC2L on formalin-fixed paraffin-embedded human spleen tissue.[antibody concentration 5ug/ml] Mouse IgG1 3E11-1G5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ORC2 antibody

Sample (30 ug of whole cell lysate). . A: Raji. . 7.5% SDS PAGE. . ORC2 antibody. . TA308075 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ORC2 antibody

ORC2L Antibody (Center) (Cat. #TA324551) western blot analysis in HUVEC cell line lysates (35ug/lane).This demonstrates the ORC2L antibody detected the ORC2L protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ORC2 antibody

Host: Rabbit; Target Name: Orc2; Sample Tissue: Mouse Lung lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu, Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

ORC2 antibody

WB using the antibody against ORC2 diluted 1:500 in PBS-Tween containing 10% milk. The position of the protein of interest is indicated on the right (expected size: 66 kDa); the marker (in kDa) is shown on the left. Mouse IgG1 Monoclol Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

ORC2 antibody

ORC2 Mouse IgG1 SB46 Purified Ye IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

ORC2 (1-577) antibody

Immunocytochemical detection of human ORC2 in HeLa with AM26604AF-N Mouse IgG1 3B7 Azide Free Hu ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

ORC2 antibody

Western blot analysis of ORC2 expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ORC2 antibody

Western blot analysis of ORC2 expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ORC2 antibody

Western blot analysis of ORC2 expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ORC2 antibody

ORC2 Antibody [3G6] Rat IgG2a 3G6 Purified Hu IP, WB
50 µl / €360.00
  Immuquest

ORC2 antibody

ORC2 Rabbit IgG Hu P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ORC2 antibody

Western Blot: Orc2 Antibody [NBP1-46175]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-ORC2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46175, which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, P, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

ORC2 antibody

ORC2 Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ORC2

ORC2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ORC2

ORC2 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

16 Item(s)

per page
  • LinkedIn