ORC4 antibody

Principal name

ORC4 antibody

Alternative names for ORC4 antibody

ORC4L, Origin recognition complex subunit 4

SwissProt ID

O43929 (Human), O88708 (Mouse), O93479 (Xenla), P54791 (Yeast), Q2YDI2 (Bovin), Q4U3P8 (Crigr), Q6P9Z8 (Rat)

Gene ID

5000 (ORC4), 2750002 (ackA)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), Dot blot (Dot), Immunoprecipitation (IP)

Background of ORC4 antibody

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown to form a core complex with ORC2L, -3L, and -5L. Three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]. Transcript Variant: This variant (2) represents the longest transcript. It differs in the 5' UTR, and encodes the same protein, as compared to variants 1 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Vashee S et al. Assembly of the human origin recognition complex. J Biol Chem 276:26666-73 (2001). PubMed PMID: 11323433
Quintana DG et al. Identification of HsORC4, a member of the human origin of replication recognition complex. J Biol Chem 272:28247-51 (1997). PubMed PMID: 9353276

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ORC4 (C-term) antibody

TA302715 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane).  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt ChIP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ORC4 antibody

WB Suggested Anti-ORC4L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Liver Rabbit IgG Purified Bov, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ORC4 antibody

Western blot analysis of ORC4L in Jurkat cell lysate with SP1346P. Goat Purified Hu WB
0.1 mg / €500.00
  Acris Antibodies GmbH

ORC4 antibody

Immunofluorescence of purified MaxPab antibody to ORC4L on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

ORC4 antibody

ORC4 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ORC4 antibody

ORC4 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

ORC4 antibody

Western Blot analysis of ORC4L expression in transfected 293T cell line (H00005000-T02) by ORC4L MaxPab polyclonal antibody.

Lane 1: ORC4L transfected lysate(50.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ORC4 antibody

Detection limit for recombinant GST tagged ORC4L is approximately 1ng/ml as a capture antibody. Mouse IgG2b 2A8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ORC4 antibody

ORC4L polyclonal antibody ( Cat # PAB6028 ) staining ( 2 µg/ml ) of Jurkat lysate ( RIPA buffer, 1.4E5 cells per lane ) . Detected by western blot using chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ORC4 antibody

Human Liver; WB Suggested Anti-ORC4L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Liver; ORC4L antibody - middle region (ARP57782_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ORC4 antibody

NB 100-798 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ORC4 antibody

Western Blot: ORC4L Antibody [NBP1-31132] - Western blot analysis of ORC4L Antibody on: A: Hela 10% SDS PAGE diluted at 1:1000 Rabbit IgG Hu Dot, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ORC4 antibody

Western Blot: ORC4L Antibody [NBP1-58167] - This Anti-ORC4L antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ORC4 (transcript variant 2)

ORC4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ORC4 (transcript variant 3)

ORC4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ORC4 (transcript variant 1)

ORC4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ORC4

ORC4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ORC4

ORC4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

ORC4L (Human) IP-WB Antibody Pair

ORC4L (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ORC4L 293T Cell Transient Overexpression Lysate(Denatured)

ORC4L 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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