Osteopontin / SPP1 antibody

Principal name

Osteopontin / SPP1 antibody

Alternative names for Osteopontin / SPP1 antibody

BNSP, OPN, SPP-1, Nephropontin, Uropontin, Secreted Phosphoprotein 1, Urinary Stone Protein, Bone Sialoprotein 1

SwissProt ID

P08721 (Rat), P10451 (Human), P10451-2, P10923 (Mouse), P14287 (Pig), P23498 (Chick), P31096 (Bovin), P31097 (Rabit), Q9XSY9 (Sheep)

Gene ID

6696 (SPP1), 25353 (Spp1), 20750, 4627 (MYH9)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Rb (Rabbit), Gt (Goat), Mky (Monkey), Gorilla

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F)

Background of Osteopontin / SPP1 antibody

Osteopontin (OPN) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors (ref 1). OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype (ref 1-4). Targeting of the gene encoding OPN, spp1 , has revealed that while OPN is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions (ref 5, 6), loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth (ref 7-12). The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN (ref 13). OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin) (ref 14). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, using inhibitory reagents, it was shown that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells, suggesting that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events (ref 14).

General readings

General / background references:Agnihotri R et al. Osteopontin, a novel substrate for matrix metalloproteinase-3 (stromelysin-1) and matrix metalloproteinase-7 (matrilysin). J Biol Chem 276:28261-7 (2001). PubMed PMID: 11375993
Ashkar S et al. Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity. Science 287:860-4 (2000). PubMed PMID: 10657301
McCawley LJ & Matrisian LM Matrix metalloproteinases: multifunctional contributors to tumor progression. Mol Med Today 6:149-56 (2000). PubMed PMID: 10740253
Ophascharoensuk V et al. Obstructive uropathy in the mouse: role of osteopontin in interstitial fibrosis and apoptosis. Kidney Int 56:571-80 (1999). PubMed PMID: 10432396
Noiri E et al. Reduced tolerance to acute renal ischemia in mice with a targeted disruption of the osteopontin gene. Kidney Int 56:74-82 (1999). PubMed PMID: 10411681
Nau GJ et al. Attenuated host resistance against Mycobacterium bovis BCG infection in mice lacking osteopontin. Infect Immun 67:4223-30 (1999). PubMed PMID: 10417195
Yoshitake H et al. Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption. Proc Natl Acad Sci U S A 96:8156-60 (1999). PubMed PMID: 10393964
Crawford HC et al. Distinct roles of osteopontin in host defense activity and tumor survival during squamous cell carcinoma progression in vivo. Cancer Res 58:5206-15 (1998). PubMed PMID: 9823334
Liaw L et al. Altered wound healing in mice lacking a functional osteopontin gene (spp1). J Clin Invest 101:1468-78 (1998). PubMed PMID: 9525990
Rittling SR et al. Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro. J Bone Miner Res 13:1101-11 (1998). PubMed PMID: 9661074
Uede T et al. Osteopontin, a coordinator of host defense system: a cytokine or an extracellular adhesive protein? Microbiol Immunol 41:641-8 (1997). PubMed PMID: 9343813
Weber GF & Cantor H The immunology of Eta-1/osteopontin. Cytokine Growth Factor Rev 7:241-8 (1996). PubMed PMID: 8971479
Denhardt DT et al. Osteopontin-induced modifications of cellular functions. Ann N Y Acad Sci 760:127-42 (1995). PubMed PMID: 7540371
Denhardt DT & Chambers AF Overcoming obstacles to metastasis--defenses against host defenses: osteopontin (OPN) as a shield against attack by cytotoxic host cells. J Cell Biochem 56:48-51 (1994). PubMed PMID: 7528752

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Osteopontin / SPP1 antibody

Immunohistochemistry: R1565 Osteopontin antibody was used at a 1/100-1/300 dilution to detect Osteopontin which is a normal component of elastic fibers in the breast (shown heavily stained in this section of Human breast tumor cells). There is also weak staining of the extracellular matrix. Osteoponin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required. Rabbit Serum Can, Hu, Ms, Por, Rt E, IP, P, WB
0.2 ml / €440.00
  Acris Antibodies GmbH

Osteopontin / SPP1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPP1 Rabbit Polyclonal antibody. (TA590059) Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806784) Mouse IgG1 OTI5E4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M07), clone 4H7. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2a 4H7 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2a 1E6 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M09), clone 3E11. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2a 3E11 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M10), clone 4F2. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2b 4F2 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M11), clone 2D12. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2b 2D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

Human breast ca. stained with Anti-Osteopontin antibody Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Osteopontin / SPP1 antibody

Human breast ca. stained with Anti-Osteopontin antibody Rabbit IgG Purified Hu P, WB
1 ml / €440.00
  Acris Antibodies GmbH

Osteopontin / SPP1 antibody

Human breast ca. stained with Anti-Osteopontin antibody Rabbit Purified Hu P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

Osteopontin / SPP1 (C-term) antibody

TA303040 staining (1µg/ml) of MOLT4 cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

Osteopontin / SPP1 (C-term) antibody

TA303040 staining (1µg/ml) of MOLT4 cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

Western Blot: Osteopontin Antibody (1B20) - Western blot analysis of Osteopontin expression in U2OS whole cell lysate. Mouse IgG1 1B20 Purified Hu, Rb, Rt C, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 (C-term) antibody

Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Goat IgG Aff - Purified Can, Hu, Ms, Rt E, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

Predicted band size: 34 kDa . Positive control: OP9 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

Predicted band size: 34 kDa . Positive control: 231 and OP9 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 231 cell lysate Lane2: OP9 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

Western blot analysis of extracts from LOVO cells, using osteopontin antibody (#TA326117).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

Western blot analysis of extracts of HeLa cell lines, using SPP1 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

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