Osteopontin / SPP1 antibody

Principal name

Osteopontin / SPP1 antibody

Alternative names for Osteopontin / SPP1 antibody

BNSP, OPN, SPP-1, Nephropontin, Uropontin, Secreted Phosphoprotein 1, Urinary Stone Protein, Bone Sialoprotein 1

SwissProt ID

P08721 (Rat), P10451 (Human), P10451-2, P10923 (Mouse), P14287 (Pig), P23498 (Chick), P31096 (Bovin), P31097 (Rabit), Q9XSY9 (Sheep)

Gene ID

6696 (SPP1), 25353 (Spp1), 20750

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Rb (Rabbit), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Osteopontin / SPP1 antibody

Osteopontin (OPN) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1. Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN. OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells. It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Weber, G.F., et al. Br. J. Cancer 103(6):861-869(2010)
Chen, R.X., et al. J. Gastroenterol. Hepatol. 25(8):1435-1442(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Osteopontin / SPP1 antibody

Immunohistochemistry: R1565 Osteopontin antibody was used at a 1/100-1/300 dilution to detect Osteopontin which is a normal component of elastic fibers in the breast (shown heavily stained in this section of Human breast tumor cells). There is also weak staining of the extracellular matrix. Osteoponin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required. Rabbit Serum Can, Hu, Ms, Por, Rt E, IP, P, WB
0.2 ml / €480.00
  Acris Antibodies GmbH

Osteopontin / SPP1 antibody

  Rabbit Polyclonal Osteopontin Antibody  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPP1 Rabbit Polyclonal antibody. (TA590059) Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  SPP1 mouse monoclonal antibody, clone OTI5E4 (formerly 5E4)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806784) Mouse IgG1 OTI5E4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M07), clone 4H7. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2a 4H7 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2a 1E6 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M09), clone 3E11. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2a 3E11 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M10), clone 4F2. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2b 4F2 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 antibody

SPP1 monoclonal antibody (M11), clone 2D12. Western Blot analysis of SPP1 expression in human stomach. Mouse IgG2b 2D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Osteopontin / SPP1 (C-term) antibody

  Goat Polyclonal Antibody against SPP1  
TA303040 staining (1µg/ml) of MOLT4 cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  Mouse Monoclonal Osteopontin Antibody (1B20)  
Western Blot: Osteopontin Antibody (1B20) - Western blot analysis of Osteopontin expression in U2OS whole cell lysate. Mouse IgG1 1B20 Purified Hu, Rb, Rt C, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 (C-term) antibody

  Osteopontin Goat Polyclonal (C-Terminus) Antibody  
Anti-Osteopontin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Goat IgG Aff - Purified Can, Hu, Ms, Rt E, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  Anti-SPP1 Rabbit Polyclonal Antibody  
Predicted band size: 34 kDa . Positive control: OP9 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  Anti-SPP1 Rabbit Polyclonal Antibody  
Predicted band size: 34 kDa . Positive control: 231 and OP9 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 231 cell lysate Lane2: OP9 cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  Rabbit polyclonal Osteopontin antibody  
Western blot analysis of extracts from LOVO cells, using osteopontin antibody (#TA326117).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  Rabbit Polyclonal SPP1 Antibody  
Western blot analysis of SPP1 in human bladder tissue lysate with SPP1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  SPP1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of HeLa cell lines, using SPP1 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  Rabbit Polyclonal Anti-Osteopontin Antibody  
Western blot analysis on LOVO cell lysate using Osteopontin Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  Rabbit Polyclonal Osteopontin Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (RC210592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  Rabbit Polyclonal Osteopontin Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (RC210592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Osteopontin / SPP1 antibody

  SPP1 mouse monoclonal antibody, clone OTI6C12 (formerly 6C12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPP1 (RC204803, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPP1. Mouse IgG1 OTI6C12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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