Otubain-1 (OTUB1) antibody

Principal name

Otubain-1 (OTUB1) antibody

Alternative names for Otubain-1 (OTUB1) antibody

Ubiquitin thioesterase OTUB1, OTB1, OTU1, HSPC263, OTU domain-containing ubiquitin aldehyde-binding protein 1, Ubiquitin-specific-processing protease OTUB1, Deubiquitinating enzyme OTUB1

SwissProt ID

B2RYG6 (Rat), Q7TQI3 (Mouse), Q96FW1 (Human)

Gene ID

55611 (OTUB1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F), Luminex (LMNX)

Background of Otubain-1 (OTUB1) antibody

Otubain 1, also known as OTUB1, is a member of the OUT (ovarian tumor) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system via its interaction with a ubiquitin protease and E3 ubiquitin ligase. This protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth. Recombinant OTUB1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Juris SJ, Shah K, Shokat K, Dixon JE, Vacratsis PO., Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry. FEBS Lett. 2006 Jan 9;580(1):179-83. (PMID: 16364312)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Otubain-1 (OTUB1) antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150) Mouse IgG1 OTI5B8 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-1 (OTUB1) antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505157) Mouse IgG1 OTI1E3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-1 (OTUB1) antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505158) Mouse IgG1 OTI2H1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-1 (OTUB1) antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505204) Mouse IgG2a OTI5F7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-1 (OTUB1) antibody

TA305680 (0.05ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . 12% SDS PAGE. TA307939 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

Western blot analysis of extracts from K562 cells, using OTUB1 Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB1 (RC210648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB1. Mouse IgG2b OTI1A6 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-1 (OTUB1) antibody

Anti-OTUB1 mouse monoclonal antibody (TA505198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OTUB1(RC210648). Mouse IgG1 OTI5F6 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 1A6 Capture (TA600424) and 2H1 Detection (TA700424) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI1A6 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 1E3 Capture (TA600425) and 5F7 Detection (TA700425) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI1E3 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 2H1 Capture (TA600426) and 5F6 Detection (TA700426) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI2H1 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 5B8 Capture (TA600427) and 1A6 Detection (TA700427) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI5B8 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 5F7 Capture (TA600428) and 1E3 Detection (TA700428) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI5F7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 1A6 Capture (TA600424) and 2H1 Detection (TA700424) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI2H1 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 1E3 Capture (TA600425) and 5F7 Detection (TA700425) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI5F7 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 2H1 Capture (TA600426) and 5F6 Detection (TA700426) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI5F6 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 5B8 Capture (TA600427) and 1A6 Detection (TA700427) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI1A6 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

OTUB1 ELISA with 5F7 Capture (TA600428) and 1E3 Detection (TA700428) Antibodies. Substrate used: Recombinant Human OTUB1 (TP310648) Mouse IgG1 OTI1E3 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

Otubain-1 (OTUB1) antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OTUB1 antibody (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : A549 cell lysate 
Lane 2. : HeLa cell lysate 
Lane 3. : HepG2 cell lysate 
Lane 4. : 293T cell lysate Mouse IgG1 AT17E10 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

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