Otubain-2 (OTUB2) antibody

Principal name

Otubain-2 (OTUB2) antibody

Alternative names for Otubain-2 (OTUB2) antibody

OTB2, OTU2, C14orf137, Ubiquitin thioesterase OTUB2, OTU domain-containing ubiquitin aldehyde-binding protein 2, Ubiquitin-specific-processing protease OTUB2, Deubiquitinating enzyme OTUB2

SwissProt ID

Q96DC9 (Human), Q9CQX0 (Mouse)

Gene ID

78990 (OTUB2)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Otubain-2 (OTUB2) antibody

OTUB2 (Ubiquitin thioesterase OTUB2) belongs to the peptidase C65 family. OTUB2 functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Recombinant human OTUB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Nanao MH, Tcherniuk SO, Chroboczek J, Dideberg O, Dessen A, Balakirev MY.
Crystal structure of human otubain 2.
EMBO Rep. 2004 Aug;5(8):783-8. Epub 2004 Jul 16
PMID: 15258613

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Otubain-2 (OTUB2) antibody

TA305685 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Otubain-2 (OTUB2) antibody

Western blot analysis of extracts from HepG2 cells, Jurkat cells, COLO cells, HT-29 cells and 293 cells, using OTUB2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Otubain-2 (OTUB2) antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OTUB2 monoclonal antibody. Mouse IgG1 OTI11A5 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-2 (OTUB2) antibody

Anti-OTUB2 mouse monoclonal antibody (TA501942) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OTUB2(RC209650). Mouse IgG1 OTI6F7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-2 (OTUB2) antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (RC209650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2. Mouse IgG1 OTI2B2 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-2 (OTUB2) antibody

Anti-OTUB2 mouse monoclonal antibody (TA501944) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OTUB2(RC209650). Mouse IgG1 OTI2F6 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-2 (OTUB2) antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (RC209650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2. Mouse IgG1 OTI11C8 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-2 (OTUB2) antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OTUB2 monoclonal antibody. Mouse IgG1 OTI11B3 Purified Can, Hu, Mky, Rt F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-2 (OTUB2) antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (RC209650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2. Mouse IgG1 OTI6C7 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-2 (OTUB2) antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (RC209650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2. Mouse IgG2a OTI2E5 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Otubain-2 (OTUB2) antibody

Otubain-2 (OTUB2) Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Otubain-2 (OTUB2) antibody

Otubain-2 (OTUB2) Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Otubain-2 (OTUB2) antibody

Otubain-2 (OTUB2) Rabbit Purified Hu, Ms, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Otubain-2 (OTUB2) antibody

Otubain-2 (OTUB2) Mouse IgG2a 2E7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Otubain-2 (OTUB2) antibody

OTUB2 polyclonal antibody ( Cat # PAB11567 ) ( 1 µg/ml ) staining of human cerebellum lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Otubain-2 (OTUB2) antibody

Western blot analysis of extracts from COLO/HepG2/Jurkat/293/HT-29 cells, using OTUB2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

Otubain-2 (OTUB2) antibody

Western blot analysis of extracts from COLO/HepG2/Jurkat/293/HT-29 cells, using OTUB2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

Otubain-2 (OTUB2) (N-term) antibody

Otubain-2 (OTUB2) Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Otubain-2 (OTUB2) antibody

Otubain-2 (OTUB2) Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Otubain-2 (OTUB2) antibody

Immunohistochemical analysis of OTUB2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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