P2RX7 / P2X7 antibody

Principal name

P2RX7 / P2X7 antibody

Alternative names for P2RX7 / P2X7 antibody

P2X purinoceptor 7, Purinergic receptor, ATP receptor, P2Z receptor

SwissProt ID

Q64663 (Rat), Q99572 (Human), Q9Z1M0 (Mouse)

Gene ID

5027 (P2RX7), 29665 (P2rx7), 18439

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of P2RX7 / P2X7 antibody

The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, med P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis. Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx alysis). With the introduction of flow cytometry, it is now possible to alyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.

General readings

Buell,GN., et al., (1998) Receptors Channels 5(6):347-54

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P2RX7 / P2X7 (N-term) antibody

TA305642 staining (0.3ug/ml) of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

P2RX7 / P2X7 antibody

Anti-P2RX7 / P2X7 antibody IHC of human pancreas, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Hu, Mky
50 µg / €345.00
  OriGene Technologies, Inc.

P2RX7 / P2X7 antibody

Western blot analysis of rat hippocampus lysates: 1. Anti-P2X7 Receptor antibody , (1:200). 2. Anti-P2X7 Receptor antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

P2RX7 / P2X7 antibody

Western blot analysis of rat brain membranes (lanes 1,5) and human cell lines; K562 (lanes 2 and 6); WEHI-231 (lanes 3 and 7) and HL-60 (lanes 4 and 8): 1-4. Anti-P2X7 Receptor (extracellular) antibody , (1:200). 5-8. Anti-P2X7 Receptor (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

P2RX7 / P2X7 antibody

WB Suggested Anti-P2RX7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

P2RX7 / P2X7 antibody

WB Suggested Anti-P2RX7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

P2RX7 / P2X7 antibody

Western blot analysis of P2RX7 in 3T3 cell lysate with P2RX7 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

P2RX7 / P2X7 antibody

HEK-293; WB Suggested Anti-P2RX7 Antibody . . Positive Control: Lane 1: 50ug mock transfected HEK-293Lane 2: 50ug hP2X7 transfected HEK-293Lane 3: 50ug mP2X7 transfected HEK-293Lane 4: 50ug rP2X7 transfected HEK-293. Primary Antibody Dilution : 1:625. Secondary Antibody : Anti-rabbit-HRP. Secondry Antibody Dilution : 1:1000. Submitted by: Ronald Sluyter, School of Biological Sciences, University of Wollongong; P2RX7 antibody - middle region (ARP35112_P050) in HEK-293 cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

P2RX7 / P2X7 antibody

Human kidney; P2RX7 antibody - N-terminal region (ARP35518_T100) in Human kidney cells using Immunohistochemistry Rabbit IgG Purified Bov, Can, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

P2RX7 / P2X7 antibody

P2RX7 / P2X7 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

Western Blot/IP/ChiP Kits

Catalog No. Host Iso. Clone Pres. React. Applications  

P2RX7 Western Blot Kit

P2RX7 Western Blot Kit Hu
25 Western Blots / kit / €640.00
  AVIVA Systems Biology

P2RX7 Western Blot kit

P2RX7 Western Blot kit
1 kit / €640.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

P2RX7 - human gene knockout kit via CRISPR

P2RX7 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

P2RX7 - human gene knockout kit via CRISPR

P2RX7 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

P2RX7 - human gene knockout kit via CRISPR

P2RX7 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

P2RX7 - human gene knockout kit via CRISPR

P2RX7 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

P2RX7 / P2X7

P2RX7 / P2X7 Human
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

P2RX7 293T Cell Transient Overexpression Lysate(Denatured)

P2RX7 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

P2RX7 overexpression lysate

P2RX7 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, P2RX7 (mGFP-tagged) - Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, P2RX7 (mGFP-tagged) - Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.

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