P2RY1 / P2Y1 antibody

Principal name

P2RY1 / P2Y1 antibody

Alternative names for P2RY1 / P2Y1 antibody

Purinergic receptor, ATP receptor

SwissProt ID

P34996 (Chick), P47900 (Human), P48042 (Bovin), P49650 (Mouse), P49651 (Rat), P59902 (Cavpo)

Gene ID

5028 (P2RY1), 25265 (P2ry1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), GP (Guinea Pig), Fe (Feline), Can (Canine), Bov (Bovine), Chk (Chicken), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Rb (Rabbit), Gorilla, Xen (Xenopus), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), ELISA (detection) (E(detection))

Background of P2RY1 / P2Y1 antibody

Purinenucletide receptor are classified in to two classes, the ionotrophic ligand-gated channels, P2X and the metabotropic G-protein coupled receptors, P2Y (1). There are 7 P2X receptors and 11 P2Y (P2Y1-2, 4, 6 and 11-14) each with unique ag9nist response profile has been identified. The subtypes o metabotropic receptors P2Y1 and P2Y2 are predominantly expressed in arterial smooth muscle. The activation of P2Y2 receptors led to release of intracellular calcium by phospholipase C action after which receptor phosphorylation appears to regulate desensitization of the P2Y2 receptor via action of protein kinase C. The purinergic receptors P2Y2 couples to the activation of mitogen activated protein kinases (MAPK) and mobilize the intracellular calcium stores, activation of phospholipase Cb, protein kinase C isofroms, focal adhesion kinases and c-Src kinases. These pathways are implicated in number of physiological and pathological processes including inflammation, neuroprotection, active astrogliosis etc., (1, 2). P2Y2 receptor induce chloride secretion in airway epithelial cells independent of cystic fibrosis trans-membrane conductance regulator and this receptor ligands are therapeutically important for the treatment of retinal detachment and improved mucociliary clearance in cystic fibrosis patients.
The P2Y2 nucleotide receptor is seven TMD GPCR family member and is coupled to a Gq protein that is stimulated equipotently by UTP and ATP, mediating activation of phospholipase C-beta (PLC-β) and mitogen-activated protein kinase (MAPK). The P2y2 undergoes rapid agonist induce desensitization and phosphorylation at Serine 243, Threonine 344 and serine 356 (1). The antagonist potencies for P2Y2 receptor is in the order of suramin>>PPADS= RB-2>TNP-ATP and suramin (pA2, 5.40) was a competitive antagonist (3). The extracellular acidification does not affect the ATP and UTP potencies of P2Y2 receptors while the P2Y4 receptor potencies are increased by 8-10-fold (3). Both P2Y2 and P2Y4 receptors regulate the UTP induced chloride secretion from intestinal mucosa and caused endothelium induced relaxation in aorta (4, 5).
P2Y2 receptor when expressed in host cells showed significant heterogeneity on SDS-PAGE mobility like many other PGCRs (6). The P2Y2 is a 377 aa protein with characteristic 7TMDs of GPCR, exhibit a broad band of 52-76 kDa (7). The P2Y receptor sequence analyses suggest the presence of putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins and has several phosphorylation sites. The Anti-P2Y2-selective antibodies were generated against conserved but unique sequences on from P2Y2 gene that are expressed only in P2Y2 and was common in several other species.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P2RY1 / P2Y1 (2 nd Extracell. Dom.) antibody

Anti-P2RY1 / P2Y1 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Chk, Elephant, Eq, GP, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt, Tr, Xen P
50 µg / €345.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 (C-term) antibody

Anti-P2RY1 / P2Y1 antibody IHC of human brain, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, GP, Hst, Hu, L, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen ICC/IF, P
50 µg / €345.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

Anti-P2RY1 / P2Y1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Hu, Mky, Ms
50 µg / €345.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P2RY1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates: 1-3. Anti-P2Y1 Receptor (extracellular) antibody, (1:200). 4-6. Anti-P2Y1 Receptor (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

Western blot analysis of rat brain membranes (1, 3) and human platelets lysate (2, 4): 1, 2. Anti-P2Y1 Receptor antibody , (1:200). 3, 4. Anti-P2Y1 Receptor antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

WB Suggested Anti-P2RY1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Fetal Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 control peptide

P2RY1 / P2Y1
0.1 mg / €250.00
  Acris Antibodies GmbH

P2RY1 / P2Y1 (C-term) antibody

P2RY1 / P2Y1 Rabbit Aff - Purified Fe, Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

P2RY1 / P2Y1 Control Peptide

P2RY1 / P2Y1 Purified
50 µg / €220.00
  Acris Antibodies GmbH

P2RY1 / P2Y1 antibody

P2RY1 / P2Y1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

P2RY1 / P2Y1 antibody

Western Blot analysis of P2RY1 expression in transfected 293T cell line (H00005028-T02) by P2RY1 MaxPab polyclonal antibody.

Lane 1: P2RY1 transfected lysate(42.10 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

P2RY1 / P2Y1 antibody

Detection limit for recombinant GST tagged P2RY1 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 4C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

P2RY1 / P2Y1 antibody

P2RY1 / P2Y1 Goat Hu, Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

P2RY1 / P2Y1 antibody

P2RY1 / P2Y1 Rabbit Hu, Ms, Rt E, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

P2RY1 / P2Y1 (Center) antibody

Rat Liver; WB Suggested Anti-P2ry1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Liver; P2ry1 antibody - middle region (ARP51290_P050) in Rat Liver cells using Western Blot Rabbit Aff - Purified Hu, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

P2RY1 / P2Y1 antibody

P2RY1 / P2Y1 Goat IgG Aff - Purified Hu E
0.1 mg / €320.00
  Novus Biologicals Inc.

P2RY1 / P2Y1 antibody

P2RY1 / P2Y1 Rabbit GP, Hu, Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

P2RY1 / P2Y1 antibody

Immunohistochemistry-Paraffin: P2Y1 Antibody [NBP1-82439] - Immunohistochemical staining of human placenta shows strong membranous and moderate cytoplasmic and nuclear positivity in trophoblastic cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

P2RY1 / P2Y1 antibody

Brain, Neurons and glia Rabbit Aff - Purified Hu P
50 µl / €440.00
  Novus Biologicals Inc.

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