P2RY1 / P2Y1 antibody

Principal name

P2RY1 / P2Y1 antibody

Alternative names for P2RY1 / P2Y1 antibody

Purinergic receptor, ATP receptor

SwissProt ID

P34996 (Chick), P47900 (Human), P48042 (Bovin), P49650 (Mouse), P49651 (Rat), P59902 (Cavpo)

Gene ID

5028 (P2RY1), 25265 (P2ry1), 18441

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline), Bt (Bat), Bov (Bovine), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), Bear, Can (Canine), Op (Opossum), L (Lizard), Ze (Zebrafish), Chimp (Chimpanzee)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of P2RY1 / P2Y1 antibody

Purinenucletide receptor are classified in to two classes, the ionotrophic ligand-gated channels, P2X and the metabotropic G-protein coupled receptors, P2Y (1). There are 7 P2X receptors and 11 P2Y (P2Y1-2, 4, 6 and 11-14) each with unique ag9nist response profile has been identified. The subtypes o metabotropic receptors P2Y1 and P2Y2 are predominantly expressed in arterial smooth muscle. The activation of P2Y2 receptors led to release of intracellular calcium by phospholipase C action after which receptor phosphorylation appears to regulate desensitization of the P2Y2 receptor via action of protein kinase C. The purinergic receptors P2Y2 couples to the activation of mitogen activated protein kinases (MAPK) and mobilize the intracellular calcium stores, activation of phospholipase Cb, protein kinase C isofroms, focal adhesion kinases and c-Src kinases. These pathways are implicated in number of physiological and pathological processes including inflammation, neuroprotection, active astrogliosis etc., (1, 2). P2Y2 receptor induce chloride secretion in airway epithelial cells independent of cystic fibrosis trans-membrane conductance regulator and this receptor ligands are therapeutically important for the treatment of retinal detachment and improved mucociliary clearance in cystic fibrosis patients.
The P2Y2 nucleotide receptor is seven TMD GPCR family member and is coupled to a Gq protein that is stimulated equipotently by UTP and ATP, mediating activation of phospholipase C-beta (PLC-β) and mitogen-activated protein kinase (MAPK). The P2y2 undergoes rapid agonist induce desensitization and phosphorylation at Serine 243, Threonine 344 and serine 356 (1). The antagonist potencies for P2Y2 receptor is in the order of suramin>>PPADS= RB-2>TNP-ATP and suramin (pA2, 5.40) was a competitive antagonist (3). The extracellular acidification does not affect the ATP and UTP potencies of P2Y2 receptors while the P2Y4 receptor potencies are increased by 8-10-fold (3). Both P2Y2 and P2Y4 receptors regulate the UTP induced chloride secretion from intestinal mucosa and caused endothelium induced relaxation in aorta (4, 5).
P2Y2 receptor when expressed in host cells showed significant heterogeneity on SDS-PAGE mobility like many other PGCRs (6). The P2Y2 is a 377 aa protein with characteristic 7TMDs of GPCR, exhibit a broad band of 52-76 kDa (7). The P2Y receptor sequence analyses suggest the presence of putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins and has several phosphorylation sites. The Anti-P2Y2-selective antibodies were generated against conserved but unique sequences on from P2Y2 gene that are expressed only in P2Y2 and was common in several other species.

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P2RY1 / P2Y1 (2 nd Extracell. Dom.) antibody

  P2RY1 / P2Y1 Rabbit Polyclonal (Extracellular Domain) Antibody  
P2RY1 / P2Y1 Rabbit Aff - Purified Bear, Bov, Bt, Chk, Elephant, Eq, GP, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt, Tr, Xen P
50 µg / €400.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 (C-term) antibody

  P2RY1 / P2Y1 Rabbit Polyclonal (C-Terminus) Antibody  
P2RY1 / P2Y1 Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, GP, Hst, Hu, L, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen ICC/IF, P
50 µg / €400.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

  P2RY1 / P2Y1 Rabbit Polyclonal (N-Terminus) Antibody  
Anti-P2RY1 / P2Y1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Hu, Mky, Ms
50 µg / €400.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

  Anti-P2RY1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P2RY1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

  Rabbit Polyclonal Anti-P2Y1 Receptor (extracellular)  
Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates: 1-3. Anti-P2Y1 Receptor (extracellular) antibody, (1:200). 4-6. Anti-P2Y1 Receptor (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

  Rabbit polyclonal Anti-P2Y1 Receptor  
Western blot analysis of rat brain membranes (1, 3) and human platelets lysate (2, 4): 1, 2. Anti-P2Y1 Receptor antibody , (1:200). 3, 4. Anti-P2Y1 Receptor antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

  Rabbit Polyclonal Anti-P2RY1  
WB Suggested Anti-P2RY1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Fetal Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

P2RY1 / P2Y1 antibody

  Rabbit Polyclonal Anti-P2RY1 Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, mouse liver tissue, Primary antibody: (P2RY1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

P2RY1 - human gene knockout kit via CRISPR

P2RY1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

P2RY1 - human gene knockout kit via CRISPR

P2RY1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

P2RY1 - human gene knockout kit via CRISPR

P2RY1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

P2RY1 - human gene knockout kit via CRISPR

P2RY1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

P2ry1 - mouse gene knockout kit via CRISPR

P2ry1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

P2ry1 - mouse gene knockout kit via CRISPR

P2ry1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

P2ry1 - mouse gene knockout kit via CRISPR

P2ry1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

P2ry1 - mouse gene knockout kit via CRISPR

P2ry1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

P2RY1 overexpression lysate

P2RY1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, P2ry1 (GFP-tagged ORF) - Rat purinergic receptor P2Y, G-protein coupled 1 (P2ry1), 200 uL, >10^7 TU/mL

Lenti ORF particles, P2ry1 (GFP-tagged ORF) - Rat purinergic receptor P2Y, G-protein coupled 1 (P2ry1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, P2ry1 (Myc-DDK-tagged ORF) - Rat purinergic receptor P2Y, G-protein coupled 1 (P2ry1), 200 uL, >10^7 TU/mL

Lenti ORF particles, P2ry1 (Myc-DDK-tagged ORF) - Rat purinergic receptor P2Y, G-protein coupled 1 (P2ry1), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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