P2RY2 / P2Y2 antibody

Principal name

P2RY2 / P2Y2 antibody

Alternative names for P2RY2 / P2Y2 antibody

P2Y purinoceptor 2, P2U purinoceptor 1, P2RU1, P2U1, Purinergic receptor, ATP receptor

SwissProt ID

O18951 (Bovin), P35383 (Mouse), P41231 (Human), P41232 (Rat), Q5YA25 (Pig)

Gene ID

5029 (P2RY2), 29597 (P2ry2), 3250292 (Q5FSN7)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of P2RY2 / P2Y2 antibody

Purinenucletide receptor are classified in to two classes, the ionotrophic ligand-gated channels, P2X and the metabotropic G-protein coupled receptors, P2Y (1). There are 7 P2X receptors and 11 P2Y (P2Y1-2, 4, 6 and 11-14) each with unique agonist response profile has been identified. The subtypes of metabotropic receptors P2Y1 and P2Y2 are predominantly expressed in arterinal smooth muscle. The activation nof P2Y2 receptors led to release of intracellular calcium by phospholipase C action after which receptor phosphorylation appears to regulate desensitization of the P2Y2 receptor via action of protein kinase C. The purinergic receptors P2Y2 couples to the activation of mitogen activated protein kinases (MAPK) and mobilize the intracellular calcium stores, activation of phospholipase Cb, protein ikinase C isofroms, focal adhesion kinases and c-Src kinases. These pathways are implicated in number of physiological and pathological processes including inflammation, neuroprotection, active astrogliosis etc (1, 2). P2Y2 receptor induce chloride secretion in airway epithelial cells independent of cystic fibrosis transmembrane conductance regulator and this receptor ligands are therapeutically important for the treatment of retinal detachment and improved mucociliary clearance in cystic fibrosis patients.
The P2Y2 nucleotide receptor is seven TMD GPCR family member and is coupled to a Gq protein that is stimulated equipotently by UTP and ATP, mediating activation of phospholipase C-beta (PLC-β) and mitogen-activated protein kinase (MAPK). The P2y2 undergoes rapid agonist induce desensitization and phosphorylation at Serine 243, threonine 344 and serine 356 (1). The antagonist potencies for P2Y2 receptor is in the order of suramin>>PPADS= RB-2>TNP-ATP and suramin (pA2, 5.40) was a competitive antagonist (3). The extracellular acidification does not affect the ATP and UTP potencies of P2Y2 receptors while the P2Y4 receptor potencies are increased by 8-10-fold (3). Both P2Y2 and P2Y4 receptors regulate the UTP induced chloride secretion from intestinal mucosa and cuased endothelium induced relaxation in aorta (4, 5).
P2Y2 receptor when expressed in host cells showed significant heterogeneity on SDS-PAGE mobility like many other PGCRs (6). The P2Y2 is a 377 aa protein with characteristic 7TMDs of GPCR, exhibit a broad band od 52-76 kDa (7). The P2Y receptor sequence analyses suggest the presence of putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins and has several phosphorylation sites.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Boucher, I., et al. Am. J. Physiol., Cell Physiol. 299 (2), C411-C421 (2010)
Ratchford, A.M., et al. J. Biol. Chem. 285(10):7545-7555(2010)
Taboubi, S., et al. Mol. Biol. Cell 21(6):946-955(2010)
Vazquez-Cuevas, F.G., et al. Reprod. Biol. Endocrinol. 8, 88 (2010)

Items 1 to 20 of 21 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P2RY2 / P2Y2 antibody

Western Blot: P2Y Purinoceptor 2 Antibody - Western blot detection of P2Y2 in 20 ug of human brain lysate (lane 2) with P2Y2 polyclonal at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. MW marker in Lane 1. Rabbit IgG Purified Hu, Ms, Rt C, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

P2RY2 / P2Y2 antibody

Predicted band size: 42 kDa . Positive control: HT-29 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

P2RY2 / P2Y2 antibody

Predicted band size: 42 kDa . Positive control: HT-29 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

P2RY2 / P2Y2 antibody

Western blot analysis of rat brain (1, 3) and lung (2, 4) membranes: 1, 2. Anti-P2Y2 Receptor antibody , (1:200). 3, 4. Anti-P2Y2 Receptor antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

P2RY2 / P2Y2 antibody

Western blot analysis of extracts of various cell lines, using P2RY2 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

P2RY2 / P2Y2 control peptide

P2RY2 / P2Y2
0.1 mg / €250.00
  Acris Antibodies GmbH

P2RY2 / P2Y2 (C-term) antibody

P2RY2 / P2Y2 Rabbit Aff - Purified Fe, Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

P2RY2 / P2Y2 (C-term) antibody

Western blot analysis of P2RY2 / P2Y2 Antibody (C-term) Cat.-No AP53144PU-N in CEM cell line lysates (35ug/lane). This demonstrates the P2RY2 antibody detected the P2RY2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

P2RY2 / P2Y2 antibody

P2RY2 / P2Y2 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

P2RY2 / P2Y2 antibody

Western Blot analysis of P2RY2 expression in transfected 293T cell line (H00005029-T02) by P2RY2 MaxPab polyclonal antibody.

Lane 1: P2RY2 transfected lysate(41.47 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

P2RY2 / P2Y2 antibody

P2RY2 / P2Y2 Rabbit Hu, Ms, Rt E, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

P2RY2 / P2Y2 Control Peptide

P2RY2 / P2Y2 Purified
20 µg / €150.00
  Neuromics Antibodies

P2RY2 / P2Y2 antibody

P2RY2 / P2Y2 Rabbit Aff - Purified Hu, Ms, Rt C, P, WB
0.1 mg / €390.00
  Neuromics Antibodies

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

P2RY2 / P2Y2

P2RY2 / P2Y2 Human
2 µg / €450.00
  Abnova Taiwan Corp.

P2RY2 / P2Y2

P2RY2 / P2Y2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

P2RY2 / P2Y2

P2RY2 / P2Y2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

P2RY2 293T Cell Transient Overexpression Lysate(Denatured)

P2RY2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

P2RY2 overexpression lysate

P2RY2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

P2RY2 overexpression lysate

P2RY2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

P2RY2 overexpression lysate

P2RY2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Items 1 to 20 of 21 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn