P4HA1 antibody

Principal name

P4HA1 antibody

Alternative names for P4HA1 antibody

P4HA, Prolyl 4-hydroxylase subunit alpha-1, EC=1.14.11.2, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1

SwissProt ID

P13674 (Human), P16924 (Chick), P54001 (Rat), Q1RMU3 (Bovin), Q60715 (Mouse)

Gene ID

5033 (P4HA1), 850333 (AGP1)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of P4HA1 antibody

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) contains an alternate in-frame exon, compared to variant 1. It encodes isoform 2, which is the same length as isoform 1. Variants 2 and 3 both encode isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI.
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
J Biol Chem. 1997 Jul 11;272(28):17342-8.
PMID: 9211872

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P4HA1 antibody

TA302527 (0.2µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

P4HA1 (431-443) antibody

Human Skin (formalin-fixed, paraffin-embedded) stained with P4HA1 antibody AP22559PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

P4HA1 antibody

Western Blot analysis of P4HA1 expression in transfected 293T cell line (H00005033-T01) by P4HA1 MaxPab polyclonal antibody.

Lane 1: P4HA1 transfected lysate(58.74 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

P4HA1 antibody

P4HA1 polyclonal antibody ( Cat # PAB7221 ) ( 0.2 µg/ml ) staining of HEK293 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

P4HA1 antibody

Western Blot: P4HA1 Antibody [NB100-57852] - NB100-57852 (0.2µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

P4HA1 antibody

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

P4HA1 antibody

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

P4HA1 (transcript variant 2)

P4HA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

P4HA1 (transcript variant 1)

P4HA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

P4HA1

P4HA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

P4HA1

P4HA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

P4HA1 293T Cell Transient Overexpression Lysate(Denatured)

P4HA1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

P4HA1 overexpression lysate

P4HA1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

P4HA1 overexpression lysate

P4HA1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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