P4HTM / PH4 antibody

Principal name

P4HTM / PH4 antibody

Alternative names for P4HTM / PH4 antibody

Transmembrane prolyl 4-hydroxylase, HIF-prolyl hydroxylase PH-4, Hypoxia-inducible factor prolyl 4-hydroxylase, HIF-PH4, HPH-4

SwissProt ID

Q8BG58 (Mouse), Q9NXG6 (Human)

Gene ID

54681 (P4HTM)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of P4HTM / PH4 antibody

HIF prolyl 4-hydroxylases (HIF-P4Hs or PHDs) are proyl hydroxylase domain-containing enzymes (PHD1, PHD2, PHD3, and P4H-TM) that mediate key physiological responses to hypoxia by modulating HIF1 alpha levels. PHD member proteins have a wide but varied tissue expression and PHD4/HIF Prolyl Hydroxylase 4 (P4H-TM) is a novel member of this family that localize to ER membrane as single-pass type II membrane protein with its catalytic site inside the lumen. P4H-TM is expressed mainly in pancreas, heart, skeletal muscle, brain, placenta, kidney and adrel gland tissues and gets induced upon hypoxic trigger. P4H-TM catalyzes the post-translatiol formation of 4-hydroxyproline in HIF alpha proteins and hydroxylates HIF1A at 'Pro-402'/'Pro-564' sites. P4H-TM has been proposed to function as a cellular oxygen sensor and, under normoxic conditions, it targets HIF through hydroxylation for proteasomal degradation via VHL/von Hippel-Lindau ubiquitition complex. Ectopic over-expression of P4H-TM in cultured neuroblastoma cells was found to reduce HIF-1alpha ODDD reporter levels, whereas its knockdown increased HIF-1alpha protein levels. HIF-P4Hs plays a critical role in several pathologies such as myocardial infarction, stroke, peripheral vascular disease, inflammation, diabetes, carcinogenesis and severe anemias.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P4HTM / PH4 antibody

Western Blot: HIF Prolyl Hydroxylase 4 Antibody [TA336302] - Whole cell lysate (25 mcg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4.  Antibody: Affinity purified rabbit anti-PHD4 (Cat. No. TA336302) used at 2 mcg/ml.  Detecti Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

ELISA: HIF Prolyl Hydroxylase 4 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

Western Blot analysis of P4HTM expression in transfected 293T cell line (H00054681-T01) by P4HTM MaxPab polyclonal antibody.

Lane 1: PH-4 transfected lysate(61.93 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

P4HTM / PH4 antibody

Western Blot analysis of PH-4 expression in transfected 293T cell line (H00054681-T01) by PH-4 MaxPab polyclonal antibody.

Lane 1: PH-4 transfected lysate(63.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

P4HTM / PH4 antibody

Western blot analysis of human PHD4 using P4HTM polyclonal antibody ( Cat # PAB12516 ). Samples : whole cell lysate from HeLa cells, normoxic and hypoxic, respectively. Rabbit Hu WB
0.1 ml / €410.00
  Abnova Taiwan Corp.

P4HTM / PH4 antibody

Human HepG2; WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; PH-4 antibody - middle region (ARP57762_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

P4HTM / PH4 antibody

Human HT1080; WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HT1080 cell lysate; PH-4 antibody - middle region (ARP57763_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

P4HTM / PH4 antibody

ELISA: HIF Prolyl Hydroxylase 4 Antibody [46020002] Rabbit Hu E, WB
50 µg / €530.00
  Novus Biologicals Inc.

P4HTM / PH4 antibody

P4HTM / PH4 Rabbit Aff - Purified Hu WB
0.1 ml / €360.00
  Novus Biologicals Inc.

P4HTM / PH4 antibody

P4HTM / PH4 Rabbit Aff - Purified Hu WB
0.1 ml / €390.00
  Novus Biologicals Inc.

P4HTM / PH4 antibody

P4HTM / PH4 Rabbit Aff - Purified Hu WB
0.1 ml / €390.00
  Novus Biologicals Inc.

P4HTM / PH4 antibody

P4HTM / PH4 Rabbit Aff - Purified Hu WB
0.1 ml / €380.00
  Novus Biologicals Inc.

P4HTM / PH4 antibody

P4HTM / PH4 Rabbit Aff - Purified Hu WB
0.1 ml / €390.00
  Novus Biologicals Inc.

P4HTM / PH4 antibody

P4HTM / PH4 Rabbit Aff - Purified Hu WB
25 µl / €140.00
  Novus Biologicals Inc.

P4HTM / PH4 antibody

Immunohistochemistry-Paraffin: P4HTM Antibody [NBP1-83989] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal and glial cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

P4HTM / PH4

P4HTM / PH4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

P4HTM / PH4

P4HTM / PH4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

P4HTM overexpression lysate

P4HTM overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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