P4HTM / PH4 antibody

Principal name

P4HTM / PH4 antibody

Alternative names for P4HTM / PH4 antibody

Transmembrane prolyl 4-hydroxylase, HIF-prolyl hydroxylase PH-4, Hypoxia-inducible factor prolyl 4-hydroxylase, HIF-PH4, HPH-4

SwissProt ID

Q8BG58 (Mouse), Q9NXG6 (Human)

Gene ID

54681 (P4HTM), 74443 (P4htm)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of P4HTM / PH4 antibody

HIF prolyl 4-hydroxylases (HIF-P4Hs or PHDs) are proyl hydroxylase domain-containing enzymes (PHD1, PHD2, PHD3, and P4H-TM) that mediate key physiological responses to hypoxia by modulating HIF1 alpha levels. PHD member proteins have a wide but varied tissue expression and PHD4/HIF Prolyl Hydroxylase 4 (P4H-TM) is a novel member of this family that localize to ER membrane as single-pass type II membrane protein with its catalytic site inside the lumen. P4H-TM is expressed mainly in pancreas, heart, skeletal muscle, brain, placenta, kidney and adrel gland tissues and gets induced upon hypoxic trigger. P4H-TM catalyzes the post-translatiol formation of 4-hydroxyproline in HIF alpha proteins and hydroxylates HIF1A at 'Pro-402'/'Pro-564' sites. P4H-TM has been proposed to function as a cellular oxygen sensor and, under normoxic conditions, it targets HIF through hydroxylation for proteasomal degradation via VHL/von Hippel-Lindau ubiquitition complex. Ectopic over-expression of P4H-TM in cultured neuroblastoma cells was found to reduce HIF-1alpha ODDD reporter levels, whereas its knockdown increased HIF-1alpha protein levels. HIF-P4Hs plays a critical role in several pathologies such as myocardial infarction, stroke, peripheral vascular disease, inflammation, diabetes, carcinogenesis and severe anemias.

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P4HTM / PH4 antibody

Western Blot: HIF Prolyl Hydroxylase 4 Antibody [TA336302] - Whole cell lysate (25 mcg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4.  Antibody: Affinity purified rabbit anti-PHD4 (Cat. No. TA336302) used at 2 mcg/ml.  Detecti Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

ELISA: HIF Prolyl Hydroxylase 4 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

Western Blot analysis of P4HTM expression in transfected 293T cell line (H00054681-T01) by P4HTM MaxPab polyclonal antibody.

Lane 1: PH-4 transfected lysate(61.93 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

P4HTM / PH4 antibody

Western Blot analysis of PH-4 expression in transfected 293T cell line (H00054681-T01) by PH-4 MaxPab polyclonal antibody.

Lane 1: PH-4 transfected lysate(63.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

P4HTM / PH4 antibody

Western blot analysis of human PHD4 using P4HTM polyclonal antibody ( Cat # PAB12516 ). Samples : whole cell lysate from HeLa cells, normoxic and hypoxic, respectively. Rabbit Hu WB
0.1 ml / €410.00
  Abnova Taiwan Corp.

P4HTM / PH4 antibody

Human HepG2; WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; PH-4 antibody - middle region (ARP57762_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

P4HTM / PH4 antibody

Human HT1080; WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HT1080 cell lysate; PH-4 antibody - middle region (ARP57763_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

P4HTM / PH4

P4HTM / PH4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

P4HTM / PH4

P4HTM / PH4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

P4HTM overexpression lysate

P4HTM overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

PH-4 293T Cell Transient Overexpression Lysate(Denatured)

PH-4 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, P4HTM (mGFP-tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, P4HTM (mGFP-tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.

Lenti ORF particles, P4HTM (Myc-DDK tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, P4HTM (Myc-DDK tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €1,000.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, P4htm (GFP-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), 200 uL, >10^7 TU/mL

Lenti ORF particles, P4htm (GFP-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.

Lenti ORF particles, P4htm (Myc-DDK-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), 200 uL, >10^7 TU/mL

Lenti ORF particles, P4htm (Myc-DDK-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), 200 uL, >10^7 TU/mL  
200 µl / €1,000.00
  OriGene Technologies, Inc.

17 Item(s)

per page
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