P4HTM / PH4 antibody

Principal name

P4HTM / PH4 antibody

Alternative names for P4HTM / PH4 antibody

Transmembrane prolyl 4-hydroxylase, HIF-prolyl hydroxylase PH-4, Hypoxia-inducible factor prolyl 4-hydroxylase, HIF-PH4, HPH-4

SwissProt ID

Q8BG58 (Mouse), Q9NXG6 (Human)

Gene ID

54681 (P4HTM), 74443 (P4htm)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of P4HTM / PH4 antibody

HIF prolyl 4-hydroxylases (HIF-P4Hs or PHDs) are proyl hydroxylase domain-containing enzymes (PHD1, PHD2, PHD3, and P4H-TM) that mediate key physiological responses to hypoxia by modulating HIF1 alpha levels. PHD member proteins have a wide but varied tissue expression and PHD4/HIF Prolyl Hydroxylase 4 (P4H-TM) is a novel member of this family that localize to ER membrane as single-pass type II membrane protein with its catalytic site inside the lumen. P4H-TM is expressed mainly in pancreas, heart, skeletal muscle, brain, placenta, kidney and adrel gland tissues and gets induced upon hypoxic trigger. P4H-TM catalyzes the post-translatiol formation of 4-hydroxyproline in HIF alpha proteins and hydroxylates HIF1A at 'Pro-402'/'Pro-564' sites. P4H-TM has been proposed to function as a cellular oxygen sensor and, under normoxic conditions, it targets HIF through hydroxylation for proteasomal degradation via VHL/von Hippel-Lindau ubiquitition complex. Ectopic over-expression of P4H-TM in cultured neuroblastoma cells was found to reduce HIF-1alpha ODDD reporter levels, whereas its knockdown increased HIF-1alpha protein levels. HIF-P4Hs plays a critical role in several pathologies such as myocardial infarction, stroke, peripheral vascular disease, inflammation, diabetes, carcinogenesis and severe anemias.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P4HTM / PH4 antibody

  Rabbit Polyclonal PHD4/HIF Prolyl Hydroxylase 4 Antibody  
Western Blot: HIF Prolyl Hydroxylase 4 Antibody [TA336302] - Whole cell lysate (25 mcg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4.  Antibody: Affinity purified rabbit anti-PHD4 (Cat. No. TA336302) used at 2 mcg/ml.  Detecti Rabbit Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

  Rabbit Polyclonal Anti-PH-4 Antibody - middle region  
WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

  Rabbit Polyclonal Anti-PH-4 Antibody - middle region  
WB Suggested Anti-PH-4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

P4HTM / PH4 antibody

  Rabbit Polyclonal PHD4/HIF Prolyl Hydroxylase 4 Antibody  
ELISA: HIF Prolyl Hydroxylase 4 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

P4HTM - human gene knockout kit via CRISPR

P4HTM - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

P4HTM - human gene knockout kit via CRISPR

P4HTM - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

P4HTM - human gene knockout kit via CRISPR

P4HTM - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

P4htm - mouse gene knockout kit via CRISPR

P4htm - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

P4htm - mouse gene knockout kit via CRISPR

P4htm - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

P4htm - mouse gene knockout kit via CRISPR

P4htm - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

P4htm - mouse gene knockout kit via CRISPR

P4htm - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

P4HTM overexpression lysate

P4HTM overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, P4HTM (mGFP-tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, P4HTM (mGFP-tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.

Lenti ORF particles, P4HTM (Myc-DDK tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, P4HTM (Myc-DDK tagged) - Human prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €1,000.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, P4htm (GFP-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), 200 uL, >10^7 TU/mL

Lenti ORF particles, P4htm (GFP-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.

Lenti ORF particles, P4htm (Myc-DDK-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), 200 uL, >10^7 TU/mL

Lenti ORF particles, P4htm (Myc-DDK-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), 200 uL, >10^7 TU/mL  
200 µl / €1,000.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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