PABPC1 antibody

Principal name

PABPC1 antibody

Alternative names for PABPC1 antibody

PAB1, PABP-1, PABP1, Polyadenylate-binding protein 1, PABPC2, Poly(A)-binding protein 1

SwissProt ID

P11940 (Human), P29341 (Mouse), P61286 (Bovin), Q9EPH8 (Rat)

Gene ID

26986 (PABPC1)

Available reactivities

Chk (Chicken), Hu (Human), Rb (Rabbit), Xen (Xenopus), Bov (Bovine), Can (Canine), Mky (Monkey), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C)

Background of PABPC1 antibody

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.

General readings

General / background references:Afonina E et al. The human poly(A)-binding protein 1 shuttles between the nucleus and the cytoplasm. J Biol Chem 273:13015-21 (1998). PubMed PMID: 9582337
Garlach M et al. The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity. Exp Cell Res 211:400-7 (1994). PubMed PMID: 7908267
Grange T et al. Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif. Nucleic Acids Res 15:4771-87 (1987). PubMed PMID: 2885805

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PABPC1 antibody

WB Suggested Anti-PABPC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PABPC1 (Center) antibody

Western blot analysis of PABPC1 Antibody (Center) (AP17616PU-N) in NIH-3T3 cell line lysates (35ug/lane). PABPC1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PABPC1 antibody

PABPC1 Rabbit Serum Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

PABPC1 antibody

Western Blot analysis of PABPC1 expression in transfected 293T cell line (H00026986-T02) by PABPC1 MaxPab polyclonal antibody.

Lane 1: PABPC1 transfected lysate(70.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PABPC1 antibody

Western blot for PABPC1 monoclonal antibody, clone 10E10 ( Cat # MAB2465 ) on HeLa cell extracts ( left lane ). Mouse IgG2b 10E10 Chk, Hu, Rb E, ICC/IF, IP, WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

PABPC1 antibody

Human brain stems (NT2); . Sample Type :. Human brain stem cells (NT2) . Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. Red: PABPC1 Blue: DAPI. Gene Name :. PABPC1. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; PABPC1 antibody - middle region (ARP40403_P050) in Human brain stems (NT2) cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PABPC1 antibody

PABP Antibody [10E10] Mouse IgG2b 10E10 Purified Chk, Hu, Rb, Xen E, F, ICC/IF, IP, WB
0.1 ml / €400.00
  Immuquest

PABPC1 antibody

PABP Antibody [10E10] Mouse IgG2b 10E10 Purified Chk, Hu, Rb, Xen E, F, ICC/IF, IP, WB
50 µl / €360.00
  Immuquest

PABPC1 antibody

PABPC1 Mouse IgG2b 10E10 Aff - Purified Bov, Can, Hu, Mky, Xen E, ICC/IF, IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

PABPC1 antibody

Western Blot: PABP Antibody [NBP1-57154] - This Anti-PABPC1 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PABPC1

PABPC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PABPC1

PABPC1 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

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