PADI4 antibody
Principal name
PADI4 antibody
Alternative names for PADI4 antibody
PDI4, PAD4, PDI5, PADI5, PADI-H, HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV
Ncbi ID
NP_036519.2, 9606, NM_012387, NP_036519, NP_035191.2, NM_011061, NM_017227
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Luminex (LMNX), ChIP assay (ChIP)
Background of PADI4 antibody
PADI4 (Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion (Nakashima et al., 1999). Suzuki et al. (2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al. (2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.
General readings
Wang Y, Wysocka J, Sayegh J, Lee YH, Perlin JR, Leonelli L, Sonbuchner LS, McDonald CH, Cook RG, Dou Y, Roeder RG, Clarke S, Stallcup MR, Allis CD, Coonrod SA. Human PAD4 regulates histone arginine methylation levels via demethylimination. Science. 2004 Oct 8;306(5694):279-83.
Western blot analysis of PADI4 polyclonal antibody ( Cat # PAB2519 ) in 293 cell line lysate. PAD4 ( arrow ) was detected using the purified polyclonal antibody.
PADI4 polyclonal antibody ( Cat # PAB6703 ) ( 0.3 µg/ml ) staining of human spleen lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of anti-PAD4 Pab (Cat.#AP12282PU-N) in 293 cell line lysate. PAD4(arrow) was detected using the purified Pab.
Western Blot analysis of PADI4 expression in transfected 293T cell line (H00023569-T02) by PADI4 MaxPab polyclonal antibody.Lane 1: PADI4 transfected lysate(74.10 KDa).Lane 2: Non-transfected lysate.
Western blot was performed on nuclear extracts from HeLa cells ( HeLa NE, 40 µg ) with PADI4 polyclonal antibody ( Cat # PAB14053 ), diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
ELISA was performed using a serial dilution of PADI4 polyclonal antibody ( Cat # PAB14053 ) in antigen coated wells.By plotting the absorbance against the crude serum dilution, the titer of the antibody was estimated to be 1 : 40,200.
Human 721 B; Host: Rabbit. Target Name: PADI4. Sample Tissue: 721_B Cell lysates. Antibody Dilution: 1.0ug/ml. There is BioGPS gene expression data showing that PADI4 is expressed in 721_B.; PADI4 antibody - middle region (ARP54888_P050) in Human 721 B cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504791)
Anti-PADI4 mouse monoclonal antibody (TA504791) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PADI4(RC206501).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813)
Anti-PADI4 mouse monoclonal antibody (TA504813) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PADI4(RC206501).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.
Flow cytometric Analysis of Hela cells, using anti-PADI4 antibody(TA504813),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PADI4 antibody(TA504813),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504814)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504814)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504814)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504887)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504887)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504887)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504887)
Anti-PADI4 mouse monoclonal antibody (TA504887) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PADI4(RC206501).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.
HEK293T cells transfected with either RC206501 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PADI4 antibody(TA504887), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504896)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504896)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504896)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504896)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504896)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504896)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504896)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4.
Flow cytometric Analysis of Hela cells, using anti-PADI4 antibody(TA504897),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PADI4 antibody(TA504897),(Red), compared to a nonspecific negative control antibody,(Blue).
PADI4 Luminex ELISA with 4H5 Capture (TA600440) and 5C10 Detection (TA700440) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501)
PADI4 Luminex ELISA with 5B7 Capture (TA600441) and 5C10 Detection (TA700440) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501)
PADI4 ELISA with 5E7 Capture (TA600442) and 4H5 Detection (TA700441) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501)
PADI4 Luminex ELISA with 5E7 Capture (TA600442) and 4H5 Detection (TA700441) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501)
PADI4 Luminex ELISA with 4H5 Capture (TA600440) and 5C10 Detection (TA700440) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501)
PADI4 Luminex ELISA with 5B7 Capture (TA600441) and 5C10 Detection (TA700440) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501)
PADI4 ELISA with 5E7 Capture (TA600442) and 4H5 Detection (TA700441) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501)
PADI4 Luminex ELISA with 5E7 Capture (TA600442) and 4H5 Detection (TA700441) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501)
TA302558 (0.3µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . 7.5% SDS PAGE. TA308535 diluted at 1:1000.
PADI4 antibody immunoprecipitates PADI4 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG. B. 5 ug of PADI4 antibody (TA308535). The precipitated DNA was detected by PCR with primer set targe
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4(TA308535) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PAD4(TA308535) antibody at 1:200 dilution.
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PADI4 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using PADI4 antibody.
Immunohistochemistry of paraffin-embedded human esophageal cancer using PADI4 antibody at dilution of 1:100 (200x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PADI4 antibody at dilution of 1:100 (200x lens).
Immunofluorescence analysis of U2OS cell using PADI4 antibody. Blue: DAPI for nuclear staining.
Immunohistochemistry: PAD4 Antibody [TA336865] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.
WB was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with the antibody against PADI4, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk is shown on the left; the location of the protein of interest is indicated on the right.
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody against PADI4. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.
- Quick-Filters:
- Highly Validated (5)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504813 | PADI4 antibody |
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| PADI4 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5) | |||||||
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Mouse | IgG2a | OTI4H5 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
 +12 additional images |
Top Selling product! Highly validated antibody!
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| TA332458 | PADI4 antibody |
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| Rabbit anti-PADI4 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Rt |
0.1 mg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA504814 | PADI4 antibody |
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| PADI4 mouse monoclonal antibody, clone OTI5E7 (formerly 5E7) | |||||||
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Mouse | IgG1 | OTI5E7 | Purified | Hu | P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
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| TA504887 | PADI4 antibody |
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| PADI4 mouse monoclonal antibody, clone OTI6A2 (formerly 6A2) | |||||||
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Mouse | IgG1 | OTI6A2 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
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| TA504896 | PADI4 antibody |
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| PADI4 mouse monoclonal antibody, clone OTI5C10 (formerly 5C10) | |||||||
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Mouse | IgG1 | OTI5C10 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
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| TA302558 | PADI4 (N-term) antibody |
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| Goat Polyclonal Antibody against PAD4 / PADI4 | |||||||
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Goat | Aff - Purified | Chimp, Hu | WB |
0.1 mg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA308535 | PADI4 antibody |
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| Rabbit Polyclonal antibody to PAD4 (peptidyl arginine deiminase, type IV) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | ChIP, ICC/IF, P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA321821 | PADI4 antibody |
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| Anti-PADI4 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA336865 | PADI4 antibody |
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| Rabbit Polyclonal PADI4 Antibody | |||||||
![Immunohistochemistry: PAD4 Antibody [TA336865] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-97309-1-H.jpg) |
Rabbit | Purified | Ms | C, P |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA347254 | PADI4 antibody |
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| Rabbit Polyclonal PADI4 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA504791 | PADI4 antibody |
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| PADI4 mouse monoclonal antibody, clone OTIE1 (formerly E1) | |||||||
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Mouse | IgG1 | OTIE1 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA504897 | PADI4 antibody |
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| PADI4 mouse monoclonal antibody, clone OTI5B7 (formerly 5B7) | |||||||
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Mouse | IgG2a | OTI5B7 | Purified | Hu | F, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA600440 | PADI4 antibody |
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| Luminex validated PADI4 mouse monoclonal antibody clone (4H5, clone OTI4H5 (formerly 4H5) | |||||||
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Mouse | IgG1 | OTI4H5 | Purified | Hu | LMNX |
0.1 mg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA600441 | PADI4 antibody |
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| Luminex validated PADI4 mouse monoclonal antibody clone (5B7, clone OTI5B7 (formerly 5B7) | |||||||
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Mouse | IgG1 | OTI5B7 | Purified | Hu | LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| TA600442 | PADI4 antibody |
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| Luminex and ELISA validated PADI4 mouse monoclonal antibody clone (5E7, clone OTI5E7 (formerly 5E7) | |||||||
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Mouse | IgG1 | OTI5E7 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA700440 | PADI4 antibody |
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| Luminex validated biotinylated PADI4 mouse monoclonal antibody (clone 5C10), clone OTI5C10 (formerly 5C10) | |||||||
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Mouse | IgG1 | OTI5C10 | Purified | Hu | LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA700441 | PADI4 antibody |
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| Luminex validated biotinylated PADI4 mouse monoclonal antibody (clone 4H5), clone OTI4H5 (formerly 4H5) | |||||||
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Mouse | IgG1 | OTI4H5 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AP12282PU-N | PADI4 (C-term) antibody |
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€370.00
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| Acris Antibodies GmbH | |||||||
| AP55150SU-N | PADI4 antibody |
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Rabbit | Serum | Hu, Ms, Rt | C, E, IP, WB |
0.2 ml /
€400.00
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| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN206501 | PADI4 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
