PADI4 antibody

Principal name

PADI4 antibody

Alternative names for PADI4 antibody

PDI4, PAD4, PDI5, PADI5, PADI-H, HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV

SwissProt ID

O88807 (Rat), Q9UM07 (Human), Q9Z183 (Mouse)

Gene ID

23569 (PADI4)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Luminex (LMNX)

Background of PADI4 antibody

PADI4 (Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion (Nakashima et al., 1999). Suzuki et al. (2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al. (2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.

General readings

Wang Y, Wysocka J, Sayegh J, Lee YH, Perlin JR, Leonelli L, Sonbuchner LS, McDonald CH, Cook RG, Dou Y, Roeder RG, Clarke S, Stallcup MR, Allis CD, Coonrod SA. Human PAD4 regulates histone arginine methylation levels via demethylimination. Science. 2004 Oct 8;306(5694):279-83.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PADI4 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504813) Mouse IgG2a OTI4H5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PADI4 antibody

Western blot analysis of extracts of various cell lines, using PADI4 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504814) Mouse IgG1 OTI5E7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PADI4 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504887) Mouse IgG1 OTI6A2 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PADI4 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504896) Mouse IgG1 OTI5C10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PADI4 (N-term) antibody

TA302558 (0.3µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Chimp, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . 7.5% SDS PAGE. TA308535 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ChIP, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

Predicted band size: 74kDa. Positive control: A431 cell lysate. Recommended dilution: 1/100-500. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PADI4 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

Immunohistochemistry: PAD4 Antibody [TA336865] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide. Rabbit Purified Ms C, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

WB was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with the antibody against PADI4, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504791) Mouse IgG1 OTIE1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PADI4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4 (RC206501, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PADI4. Mouse IgG2a OTI5B7 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PADI4 antibody

PADI4 Luminex ELISA with 4H5 Capture (TA600440) and 5C10 Detection (TA700440) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501) Mouse IgG1 OTI4H5 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

PADI4 Luminex ELISA with 5B7 Capture (TA600441) and 5C10 Detection (TA700440) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501) Mouse IgG1 OTI5B7 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

PADI4 ELISA with 5E7 Capture (TA600442) and 4H5 Detection (TA700441) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501) Mouse IgG1 OTI5E7 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

PADI4 antibody

PADI4 Luminex ELISA with 4H5 Capture (TA600440) and 5C10 Detection (TA700440) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501) Mouse IgG1 OTI5C10 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

PADI4 antibody

PADI4 ELISA with 5E7 Capture (TA600442) and 4H5 Detection (TA700441) Antibodies. Substrate used: Recombinant Human PADI4 (TP306501) Mouse IgG1 OTI4H5 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

PADI4 (C-term) antibody

Western blot analysis of anti-PAD4 Pab (Cat.#AP12282PU-N) in 293 cell line lysate. PAD4(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PADI4 antibody

PADI4 Rabbit Serum Hu, Ms, Rt C, E, IP, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Items 1 to 20 of 38 total

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