PAFAH1B1 / LIS1 antibody

Principal name

PAFAH1B1 / LIS1 antibody

Alternative names for PAFAH1B1 / LIS1 antibody

PAF-AH alpha, PAFAH alpha, PAFAHA, Lissencephaly-1 protein, LIS-1, MDCR, MDS, Platelet-activating factor acetylhydrolase IB subunit alpha, PAF acetylhydrolase 45 kDa subunit

SwissProt ID

P43033 (Bovin), P43034 (Human), P63004 (Rat), P63005 (Mouse), Q9GL51 (Pig), Q9PTR5 (Chick)

Gene ID

5048 (PAFAH1B1), 18472 (Pafah1b1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of PAFAH1B1 / LIS1 antibody

This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 is the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.PAFAH1B1 was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordites used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene.

General readings

Reiner O, Bar-Am I, Sapir T, Shmueli O, Carrozzo R, Lindsay EA, Baldini A, Ledbetter DH, Cahana A.
LIS2, gene and pseudogene, homologous to LIS1 (lissencephaly 1), located on the short and long arms of chromosome 2.
Genomics. 1995 Nov 20;30(2):251-6.
PMID: 8586424

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAFAH1B1 / LIS1 (C-term) antibody

TA302796 staining (2µg/ml) of NIH-3T3 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

Western blot analysis of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

Predicted cell location: Cytoplasm. Positive control: Human lung and liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human lung and liver cancer tissue using PAFAH1B1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

Predicted cell location: Cytoplasm. Positive control: Human ovarian and brain tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human ovarian and brain tissue using PAFAH1B1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

WB Suggested Anti-PAFAH1B1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysateThere is BioGPS gene expression data showing that PAFAH1B1 is expressed in MCF7 Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

WB Suggested Anti-PAFAH1B1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

Thymus: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PAFAH1B1 / LIS1 (397-410) antibody

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with PAFAH1B1 antibody AP22516PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fe, Fi, Hst, Hu, Insect, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PAFAH1B1 / LIS1 Control Peptide

PAFAH1B1 / LIS1
50 µg / €160.00
  Acris Antibodies GmbH

PAFAH1B1 / LIS1 antibody

PAFAH1B1 / LIS1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PAFAH1B1 / LIS1 antibody

Detection limit for recombinant GST tagged PAFAH1B1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 2C12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAFAH1B1 / LIS1 antibody

Western Blot analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5.

Lane 1: PAFAH1B1 transfected lysate(46.6 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 5A5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAFAH1B1 / LIS1 antibody

PAFAH1B1 polyclonal antibody ( Cat # PAB6171 ) ( 0.1µg/ml ) staining of rat ovary lysate ( 35µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PAFAH1B1 / LIS1 antibody

Western blot analysis of PAFAH1B1 in HeLa cell lysate with PAFAH1B1 polyclonal antibody ( Cat # PAB13006 ) at ( A ) 0.5 and ( B ) 1 µg/ml. Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PAFAH1B1 / LIS1 antibody

Human MCF-7; WB Suggested Anti-PAFAH1B1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate. There is BioGPS gene expression data showing that PAFAH1B1 is expressed in MCF7.; PAFAH1B1 antibody - N-terminal region (ARP56107_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PAFAH1B1 / LIS1 antibody

Human Lung; WB Suggested Anti-PAFAH1B1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Lung; PAFAH1B1 antibody - N-terminal region (ARP56108_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PAFAH1B1 / LIS1 antibody

PAFAH1B1 / LIS1 Mouse IgG1 PAFAH-8B1 Purified Hu ELISA, WB
50 µg / €640.00
  Funakoshi Co., Ltd.

PAFAH1B1 / LIS1 antibody

PAFAH1B1 / LIS1 Mouse IgG1 PAFAH-A7G Purified Hu ELISA, WB
50 µg / €640.00
  Funakoshi Co., Ltd.

PAFAH1B1 / LIS1 antibody

PAFAH1B1 / LIS1 Mouse PAFAH-A7G Biotin Hu ELISA, WB
50 µg / €760.00
  Funakoshi Co., Ltd.

PAFAH1B1 / LIS1 antibody

Western Blot: LIS1 / PAFAH1B1 Antibody [NB100-1040]-(0.1µg/ml) staining of Rat Ovary lysate (35µg protein in RIPA buffer). Goat IgG Aff - Purified Hu, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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