PAFAH1B1 / LIS1 antibody

Principal name

PAFAH1B1 / LIS1 antibody

Alternative names for PAFAH1B1 / LIS1 antibody

PAF-AH alpha, PAFAH alpha, PAFAHA, Lissencephaly-1 protein, LIS-1, MDCR, MDS, Platelet-activating factor acetylhydrolase IB subunit alpha, PAF acetylhydrolase 45 kDa subunit

SwissProt ID

P43033 (Bovin), P43034 (Human), P63004 (Rat), P63005 (Mouse), Q9GL51 (Pig), Q9PTR5 (Chick)

Gene ID

5048 (PAFAH1B1), 18472 (Pafah1b1), 83572

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Bt (Bat), Eq (Equine), Fe (Feline), Fi (Fish), Hst (Hamster), Insect, Mky (Monkey), Op (Opossum), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), Bear

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PAFAH1B1 / LIS1 antibody

This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 is the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.PAFAH1B1 was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordites used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene.

General readings

Reiner O, Bar-Am I, Sapir T, Shmueli O, Carrozzo R, Lindsay EA, Baldini A, Ledbetter DH, Cahana A.
LIS2, gene and pseudogene, homologous to LIS1 (lissencephaly 1), located on the short and long arms of chromosome 2.
Genomics. 1995 Nov 20;30(2):251-6.
PMID: 8586424

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAFAH1B1 / LIS1 (C-term) antibody

  Goat Polyclonal Antibody against PAFAH1B1  
TA302796 staining (2µg/ml) of NIH-3T3 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

  Rabbit Polyclonal LIS1 Antibody  
Western blot analysis of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

  Anti-PAFAH1B1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human lung and liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human lung and liver cancer tissue using PAFAH1B1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

  Anti-PAFAH1B1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human ovarian and brain tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human ovarian and brain tissue using PAFAH1B1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

  Rabbit polyclonal Anti-PAFAH1B1 Antibody  
WB Suggested Anti-PAFAH1B1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysateThere is BioGPS gene expression data showing that PAFAH1B1 is expressed in MCF7 Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

  Rabbit polyclonal Anti-PAFAH1B1 Antibody  
WB Suggested Anti-PAFAH1B1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAFAH1B1 / LIS1 antibody

Thymus: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PAFAH1B1 / LIS1 (397-410) antibody

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with PAFAH1B1 antibody AP22516PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fe, Fi, Hst, Hu, Insect, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PAFAH1B1 - human gene knockout kit via CRISPR

PAFAH1B1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PAFAH1B1 - human gene knockout kit via CRISPR

PAFAH1B1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PAFAH1B1 - human gene knockout kit via CRISPR

PAFAH1B1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PAFAH1B1 - human gene knockout kit via CRISPR

PAFAH1B1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pafah1b1 - mouse gene knockout kit via CRISPR

Pafah1b1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pafah1b1 - mouse gene knockout kit via CRISPR

Pafah1b1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pafah1b1 - mouse gene knockout kit via CRISPR

Pafah1b1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pafah1b1 - mouse gene knockout kit via CRISPR

Pafah1b1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PAFAH1B1 / LIS1

  Recombinant protein of human platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa (PAFAH1B1)  
PAFAH1B1 / LIS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PAFAH1B1 (mGFP-tagged) - Human platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) (PAFAH1B1), 200 uL, >10^7 TU/mL

Lenti ORF particles, PAFAH1B1 (mGFP-tagged) - Human platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) (PAFAH1B1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PAFAH1B1 (Myc-DDK tagged) - Human platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) (PAFAH1B1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PAFAH1B1 (Myc-DDK tagged) - Human platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) (PAFAH1B1) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pafah1b1 (GFP-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit (cDNA clone MGC:13913 IMAGE:4017963),, 200 uL, >10^7 TU/mL

Lenti ORF particles, Pafah1b1 (GFP-tagged) - Mouse platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit (cDNA clone MGC:13913 IMAGE:4017963),, 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.

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