PAK1 antibody

Principal name

PAK1 antibody

Alternative names for PAK1 antibody

PAK 1, PAK-1, Alpha-PAK, PAK alpha, p21-activated kinase 1, p65-PAK, Serine/threonine-protein kinase PAK 1

SwissProt ID

O88643 (Mouse), P35465 (Rat), P38990 (Yeast), Q08E52 (Bovin), Q13153 (Human), Q17850 (Caeel)

Gene ID

5058 (PAK1), 29431 (Pak1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of PAK1 antibody

A brain serine threonine protein kinase is a target for p21 ras related proteins Cdc42 and Rac1. p21-activated kinases (PAKs) bind to and are activated by Rho family GTPases such as Cdc42 and Rac. These GTPases play key roles in regulating cell polarity, stress responses, and cell cycle progression (1). Stiochiometeric analyses using purified Rac and PAK suggest that Rac binds to PAK with high affinity in a 1:1 ratio. The kinase complexes specifically with aGTP bound activated p21, inhibiting the p21 activity and leading to activation and auto-phosphorylation of p65pak (2). Three isoforms of p21-activated serine/threonine kinases, PAK68, PAK65 and PAK62, were found in both cytosolic & membrane fraction, & their contents were significantly increased during induced retinoic acid differentiation of human promyelocytic leukemia HL60 cell (3). The content of PAK65 or Rac is not evident in plasma membrane fraction of cells form un-differentiated cells (2).
Constitutively activated Pak1 also suppress the activity of scavenger receptor class B, type 1 (SB-B1) in macrophage cell line RAW264.7, an action similar to LPS (4). Mutational studies with PAK1 also suggest that SR-B1 receptor down regulation involves binding to Rac and Cdc42. In mammalian cells, hyper osmotic shocks can induce apoptotic cell death. It is suggested that hyper osmotic shock can induce cleavage for PAK2 to produce a 36kDa kinase, which is the result of DNA fragmentation and activation of ICE/CED-3 family cysteine proteases termed caspases-3.

General readings

1、 Brown JL, Stowers L, Baer M, Trejo J, Coughlin S, Chant J (Mar 1997). "Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway". Curr Biol 6 (5): 598–605.
2、 Bekri S, Adelaide J, Merscher S, Grosgeorge J, Caroli-Bosc F, Perucca-Lostanlen D, Kelley PM, Pebusque MJ, Theillet C, Birnbaum D, Gaudray P (Apr 1998). "Detailed map of a region commonly amplified at 11q13-->q14 in human breast carcinoma". Cytogenet Cell Genet 79 (1–2): 125–31.
3、"Entrez Gene: PAK1 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)".
4、 Bekri, S., Adelaide, J., Merscher, S., Grosgeorge, J., Caroli-Bosc, F., Perucca-Lostanlen, D., Kelley, P. M., Pebusque, M.-J., Theillet, C., Birnbaum, D., Gaudray, P. Detailed map of a region commonly amplified at 11q13-q14 in human breast carcinoma. Cytogenet. Cell Genet. 79: 125-131, 1997.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAK1 antibody

  Goat Polyclonal Antibody against PAK1  
TA303131 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Anti-PAK1 (Phospho-Thr212) Rabbit Polyclonal Antibody  
Predicted band size: 68 kDa . Positive control: 293 cells untreated or treated with forskolin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with forskolin lysate Lane2: 293 cells treated with forskolin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Anti-PAK1 Rabbit Polyclonal Antibody  
Predicted band size: 61 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Anti-PAK1 Rabbit Polyclonal Antibody  
Predicted band size: 61 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Rabbit Polyclonal PAK1 Antibody  
Western blot analysis of PAK1 expression in Etoposide treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Rabbit polyclonal PAK1/2/3 (Ab-423/402/421) antibody  
Western blot analysis of extracts from NIH-3T3 cells, using PAK1/2/3 (Ab-423/402/421) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Rabbit polyclonal PAK1 (Ab-204) antibody  
Western blot analysis of extracts from 3T3 cells, treated with UV (15mins), using PAK1 (Ab-204) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Rabbit polyclonal PAK1 (Phospho-Ser199) antibody  
Western blot analysis of extracts from LOVO cells, treated with starved (24hours), using PAK1/2 (Phospho-Ser199) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Rabbit polyclonal PAK1/2 (Ab-199) antibody  
Western blot analysis of extracts from K562 cells, using PAK1/2 (Ab-199) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  PAK1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of mouse kidney cell line, using PAK1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

  Rabbit Polyclonal anti-PAK alpha Antibody  
PAK1 Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK1 antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1E11 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

PAK1 antibody

PAK1 Rabbit Purified Hu IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

PAK1 pThr212 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 (phospho-Thr212) antibody (AP02438PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PAK1 pThr212 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 (phospho-Thr212) antibody (AP02438PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

PAK1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 antibody AP02681PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PAK1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 antibody AP02681PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

PAK1 antibody

Immunohistochemistry (IHC) analyzes of PAK1 antibody (Cat.-No: AP06555PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PAK1 pThr212 antibody

Dot blot analysis of Phospho-PAK1-T212 Pab (Cat#AP12780PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

PAK1 pThr423 antibody

Dot blot analysis of anti-PAK1-pThr423 Phospho-specific Pab (Cat.#AP12836PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

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