PAK4 antibody

Principal name

PAK4 antibody

Alternative names for PAK4 antibody

PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, Serine/threonine-protein kinase PAK 4

SwissProt ID

O96013 (Human), Q8BTW9 (Mouse)

Gene ID

10298 (PAK4), 70584 (Pak4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Can (Canine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PAK4 antibody

The p21-activated kises (PAKs) are serine-threonine kises that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neurol development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

General readings

Liu Y., et al. (2008). Mol Cancer Res. 6(7):1215-24.
Li X., et al. (2005). J Biol Chem. 280(50):41192-200.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAK4 antibody

  PAK4 mouse monoclonal antibody, clone OTI1C7 (formerly 1C7)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PAK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807297) Mouse IgG1 OTI1C7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAK4 antibody

  Rabbit Polyclonal PAK4 Antibody  
Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAK4 antibody

  Anti-PAK4 Rabbit Polyclonal Antibody  
Predicted band size: 64 kDa . Positive control: Human colon cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK4 antibody

  Rabbit Polyclonal Anti-PAK4 Antibody  
Host: Rabbit; Target Name: PAK4; Sample Tissue: ACHN Whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAK4 antibody

  PAK4 mouse monoclonal antibody, clone OTI1C11 (formerly 1C11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4 (RC202302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAK4. Mouse IgG1 OTI1C11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAK4 antibody

  PAK4 mouse monoclonal antibody, clone OTI3C3 (formerly 3C3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4 (RC202302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAK4. Mouse IgG1 OTI3C3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAK4 (incl. pos. control) antibody

Detection of endogenous PAK4: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20217PU-N PAK4 antibody (Clone 6C1) at 0.5 µg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431 
Lane 2:SW480 
Lane 3: SW620
Lane 4: HT29 
Lane 5: MCF7 
Lane 6: MDA-MB-231 
Lane 7: T47D Mouse IgG1 6C1 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

PAK4 (N-term) antibody

PAK4 Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PAK4 antibody

Figure 2. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PAK4 (N-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.  (RIGHT)Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PAK4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PAK4 antibody

Western blot analysis of extracts from K562 cells, treated with PMA 125ng/ml 30', using PAK4/5/6 (Ab-474) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

PAK4 antibody

Western blot analysis of extracts from K562 cells, treated with PMA 125ng/ml 30', using PAK4/5/6 (Ab-474) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

PAK4/PAK5/PAK6 antibody

Western blot (WB) analysis of PAK4/5/6 antibody (Cat.-No.: AP01447PU-N) in extracts from K562 cell treated with PMA 125ng/ml 30'. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PAK4 - human gene knockout kit via CRISPR

PAK4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PAK4 - human gene knockout kit via CRISPR

PAK4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PAK4 - human gene knockout kit via CRISPR

PAK4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PAK4 - human gene knockout kit via CRISPR

PAK4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pak4 - mouse gene knockout kit via CRISPR

Pak4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pak4 - mouse gene knockout kit via CRISPR

Pak4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pak4 - mouse gene knockout kit via CRISPR

Pak4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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