PAK4 antibody

Principal name

PAK4 antibody

Alternative names for PAK4 antibody

PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, Serine/threonine-protein kinase PAK 4

SwissProt ID

O96013 (Human), Q8BTW9 (Mouse)

Gene ID

10298 (PAK4), 1029856 (atpB), 70584 (Pak4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Can (Canine), Hst (Hamster), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of PAK4 antibody

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

General readings

Liu Y., et al. (2008). Mol Cancer Res. 6(7):1215-24.
Li X., et al. (2005). J Biol Chem. 280(50):41192-200.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAK4 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PAK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807297) Mouse IgG1 OTI1C7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAK4 antibody

Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAK4 antibody

Predicted band size: 64 kDa . Positive control: Human colon cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAK4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4 (RC202302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAK4. Mouse IgG1 OTI1C11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAK4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAK4 (RC202302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAK4. Mouse IgG1 OTI3C3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAK4 (incl. pos. control) antibody

Detection of endogenous PAK4: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20217PU-N PAK4 antibody (Clone 6C1) at 0.5 µg/ ml for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431 
Lane 2:SW480 
Lane 3: SW620
Lane 4: HT29 
Lane 5: MCF7 
Lane 6: MDA-MB-231 
Lane 7: T47D Mouse IgG1 6C1 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

PAK4 (N-term) antibody

PAK4 Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PAK4 antibody

Figure 2. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PAK4 (N-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.  (RIGHT)Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PAK4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PAK4 antibody

PAK4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PAK4 antibody

PAK4 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

PAK4 antibody

PAK4 Mouse IgG1 3F10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAK4 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PAK4 polyclonal antibody ( Cat # PAB2301 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PAK4 pSer474 antibody

The cell lysate derived from HEK 293 cells was immunoprobed by PAK4 ( phospho S474 ) polyclonal antibody ( Cat # PAB12642 ) at 1 : 500. An immunoreactive band is observed around ~ 68kDa ( Lane 1 ). This band is blocked by pre-incubation with immunizing peptide ( Lane 2 ). Rabbit Bov, Chk, Hu, Ms, Rt E, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PAK4 antibody

The cell lysate derived from 293 cells was immunoprobed by PAK4 polyclonal antibody ( Cat # PAB12721 ) at 1 : 500.
An immunoreactive band is observed around ~68kDa ( lane 1 ).
This band is blocked by pre-incubation with immunizing peptide ( Lane 2 ). Rabbit Bov, Chk, Hu, Ms, Rt E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PAK4 antibody

Immunohistochemistry of PAK4 in human colon tissue with PAK4 polyclonal antibody ( Cat # PAB12892 ) at 10 µg/ml. Rabbit Hu P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PAK4 antibody

Western blot analysis of extracts from K562 cells, treated with PMA 125ng/ml 30', using PAK4/5/6 (Ab-474) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

PAK4 antibody

Western blot analysis of extracts from K562 cells, treated with PMA 125ng/ml 30', using PAK4/5/6 (Ab-474) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

PAK4 pSer474 antibody

Immunofluorescent analysis of PAK4 (pS474) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

PAK4 pSer474 antibody

Immunofluorescent analysis of PAK4 (pS474) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

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