PAM antibody

Principal name

PAM antibody

Alternative names for PAM antibody

PHM, PAL, Peptidyl-glycine alpha-amidating monooxygenase, Peptidylglycine alpha-hydroxylating monooxygenase, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase

SwissProt ID

P10731 (Bovin), P14925 (Rat), P19021 (Human), P97467 (Mouse)

Gene ID

5066 (PAM), 18484 (Pam), 25508

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of PAM antibody

PAM is a multifunctiol protein. It has two enzymatically active domains with catalytic activities-peptidylglycine alpha-hydroxylating monooxygese (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products.This gene encodes a multifunctiol protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygese (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple altertively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known.

General readings

Glauder J, Ragg H, Rauch J, Engels JW.
Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells.
Biochem Biophys Res Commun. 1990 Jun 15;169(2):551-8.
PMID: 2357221

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAM (C-term) antibody

  Goat Polyclonal Antibody against PAM  
TA303337 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAM antibody

  Rabbit Polyclonal Anti-PAM Antibody  
WB Suggested Anti-PAM Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 721_B cell lysate Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

PAM - human gene knockout kit via CRISPR

PAM - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PAM - human gene knockout kit via CRISPR

PAM - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PAM - human gene knockout kit via CRISPR

PAM - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PAM - human gene knockout kit via CRISPR

PAM - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pam - mouse gene knockout kit via CRISPR

Pam - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pam - mouse gene knockout kit via CRISPR

Pam - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pam - mouse gene knockout kit via CRISPR

Pam - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pam - mouse gene knockout kit via CRISPR

Pam - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PAM (transcript variant 3)

  Recombinant protein of human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 3  
PAM Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

PAM overexpression lysate

PAM overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

PAM overexpression lysate

PAM overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

PAM-knockout Cell lysate

PAM-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €2,010.00
  OriGene Technologies, Inc.

Lenti ORF particles, PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,890.00
  OriGene Technologies, Inc.

Lenti ORF particles, PAM (mGFP-tagged) - Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, PAM (mGFP-tagged) - Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €1,870.00
  OriGene Technologies, Inc.

Lenti ORF particles, PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 5, 200 uL, >10^7 TU/mL

Lenti ORF particles, PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 5, 200 uL, >10^7 TU/mL  
200 µl / €2,000.00
  OriGene Technologies, Inc.

Lenti ORF particles, PAM (Myc-DDK-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PAM (Myc-DDK-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,810.00
  OriGene Technologies, Inc.

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