PAPOLA antibody

Principal name

PAPOLA antibody

Alternative names for PAPOLA antibody

PAP-alpha, Poly(A) polymerase alpha, Polynucleotide adenylyltransferase alpha

SwissProt ID

P25500 (Bovin), P51003 (Human), Q61183 (Mouse)

Gene ID

10914 (PAPOLA), 1091412 (ackA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PAPOLA antibody

Polyadenylation of the 3-prime ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction includes endoribonucleolytic cleavage of the pre-RNA at the poly(A) site that leads to synthesis of the poly(A) tail at the 3-prime end of the upstream cleavage product. The poly(A) polymerase (PAP) is required The adenosine addition reaction depends on poly(A) polymerase (PAP) activity. The testis express PAP-beta (TPAP) in the cytoplasm of spermatogenic cells. The adenosine addition function of PAP-beta plays a critical role in male germ cell production. PAP-beta-deficient transgenic mice display impaired expression of haploid-specific genes that are necessary for spermatogenesis. The intronless gene encoding human PAP-beta maps to chromosome 7p22.3.

General readings

Rapti, A., et al. Mol. Cell. Biochem. 340 (1-2), 91-96 (2010)
Nan, H., et al. J. Invest. Dermatol. 129(9):2250-2257(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Dettwiler, S., et al. J. Biol. Chem. 279(34):35788-35797(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAPOLA antibody

WB using the antibody against PAPOLA diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAPOLA (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma reacted with AP53159PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PAPOLA antibody

Western Blot and Immunoprecipitation: PAPOLA Antibody [NB100-55267] - Detection of Human PAPOLA on HeLa whole cell lysate using NB100-55267. PAPOLA was also immunoprecipitated by rabbit anti-PAPOLA antibody NB100-55268. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

PAPOLA antibody

Western Blot and Immunoprecipitation: PAPOLA Antibody [NB100-55268] - Detection of Human PAPOLA on HeLa whole cell lysate using NB100-55268. PAPOLA was also immunoprecipitated by rabbit anti-PAPOLA antibody NB100-55267. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

PAPOLA antibody

Immunohistochemistry-Paraffin: PAPOLA Antibody [NBP1-87345] - Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PAPOLA

PAPOLA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PAPOLA

PAPOLA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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