PAPOLG antibody

Principal name

PAPOLG antibody

Alternative names for PAPOLG antibody

PAP2, PAPG, PAP-gamma, Neo-PAP, Poly(A) polymerase gamma, Neo-poly(A) polymerase, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme

SwissProt ID

Q6PCL9 (Mouse), Q9BWT3 (Human)

Gene ID

64895 (PAPOLG)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of PAPOLG antibody

PAPOLG is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3\' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity.This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3\' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.

General readings

Beausoleil,S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAPOLG antibody

WB Suggested Anti-PAPOLG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAPOLG antibody

Human HeLa; WB Suggested Anti-PAPOLG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate; PAPOLG antibody - middle region (ARP40991_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PAPOLG antibody

WB of IP: PAPOLG Antibody [NBP1-30060] - WCL from HeLa cells. NBP1-30060 used for IP at 10 mcg/mg lysate. PAP-gamma was also IPed by rabbit anti-PAP-gamma antibody NBP1-30061. For blotting IPed PAP-gamma, NBP1-30061 was used at 1 mcg/ml. Rabbit Aff - Purified Hu IP
0.1 ml / €470.00
  Novus Biologicals Inc.

PAPOLG antibody

WB and IP: PAPOLG Antibody [NBP1-30061] -Samples: Whole cell lysate from HeLa and mouse NIH3T3 cells. PAP-gamma was also IPed by NBP1-30060. For blotting IPed PAP-gamma, the ReliaBLOT®  Reagents and Procedures were used. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PAPOLG antibody

Western Blot: PAPOLG Antibody [NBP1-57230] - This Anti-PAPOLG antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

PAPOLG overexpression lysate

PAPOLG overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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