PAPSS1 antibody

Principal name

PAPSS1 antibody

Alternative names for PAPSS1 antibody

ATPSK1, PAPSS, PAPSS 1, Sulfurylase kinase 1, SK1, Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1

SwissProt ID

O43252 (Human), O54820 (Cavpo), Q60967 (Mouse)

Gene ID

9061 (PAPSS1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of PAPSS1 antibody

Sulfotransferase (SULT) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (PAPSS) catalyzes the biosynthesis of PAPS which serves as the universal sulfonate donor compound for all sulfotransferase reactions. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (603005). Bifunctional PAPSS1 is comprised of an N-terminal APS kinase domain, and a C-terminal ATP sulfurylase domain. Full-length protein has significantly less APS kinase activity than the N-terminal fragment, suggesting that the C-terminal domain exerts a regulatory role on the N-terminal APS kinase activity. In humans there are two major isoforms: PAPSS1 and PAPSS2. In brain and skin PAPSS1 is the major isoform, whereas in liver, cartilage and adrenal glands PAPSS2 isoform expression dominates. The predicted 623-amino acid protein is 98% identical to mouse PAPS synthase. The N-terminal 268-amino acid region of human PAPS synthase resembles APS kinases from other organisms and contains 3 conserved nucleotide-binding motifs.

General readings

Girard J.-P., et al. (1998) FASEB J. 12:603-612Venkatachalam K.V., et al. (1999) J. Biol. Chem. 274:2601-2604

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAPSS1 (C-term) antibody

Western blot analysis of anti-PAPSS1 Antibody (C-term) (Cat.#AP12297PU-N) in A2058 cell line lysates (35ug/lane). PAPSS1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PAPSS1 antibody

PAPSS1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PAPSS1 antibody

Western blot analysis of PAPSS1 over-expressed 293 cell line, cotransfected with PAPSS1 Validated Chimera RNAi ( Cat # H00009061-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PAPSS1 monoclonal antibody (M05), clone 1F4 (Cat # H00009061-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG2a 1F4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAPSS1 antibody

Western blot analysis of PAPSS1 polyclonal antibody ( Cat # PAB2534 ) in HepG2 cell line lysate ( 35 µg/lane ) . PAPSS1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 45 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PAPSS1 antibody

Western  Blot:  PAPSS1 Antibody [NBP1-33470] – Western blot analysis of PAPSS1 on Hep G2 lysates using NBP1-33470 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PAPSS1

PAPSS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PAPSS1 (24-624, His-tag)

PAPSS1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

PAPSS1 (24-624, His-tag)

PAPSS1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

PAPSS1

PAPSS1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PAPSS1

PAPSS1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

PAPSS1

PAPSS1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PAPSS1

PAPSS1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

12 Item(s)

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