PAR4 / F2RL3 antibody

Principal name

PAR4 / F2RL3 antibody

Alternative names for PAR4 / F2RL3 antibody

Proteinase-activated receptor 4, PAR-4, Thrombin receptor-like 3, Coagulation factor II receptor-like 3

SwissProt ID

O88634 (Mouse), Q920E0 (Rat), Q96RI0 (Human)

Gene ID

9002 (F2RL3), 116498 (F2rl3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Eq (Equine), Mky (Monkey), Por (Porcine), Can (Canine), Bear

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of PAR4 / F2RL3 antibody

Protease-activated receptor 4 (PAR-4) belongs to a family of four G protein-coupled receptors (PAR-1 - 4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their me. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-termil domain. This results in the creation of a new N-termil tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several sigl transduction pathways. Different PARs are activated by different proteases. Hence, PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated only by thrombin and PAR-2 is activated only by trypsin. PAR-4 can be also cleaved and activated by other proteases such as cathepsin G. The intracellular sigling mechanisms mediated by PAR-4 activation are not completely elucidated but they involve calcium mobilization downstream of phospholipase Cβ through the Gαq pathway. Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived form PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation. PAR-4 is likely involved in other physiological functions such as regulation of gastrointestil motility and regulation of vascular endothelial cell function.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAR4 / F2RL3 (N-term) antibody

  Rabbit Polyclonal Anti-F2RL3 Antibody (N-Terminus)  
Anti-F2RL3 / PAR4 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bov, Elephant, Hu, Mky, Ms, Por, Rt P
50 µg / €400.00
  OriGene Technologies, Inc.

PAR4 / F2RL3 antibody

  Rabbit Polyclonal Anti-Protease-activated Receptor-4 (extracellular)  
Western blot analysis of rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates: 1, 2. Anti-Protease-Activated Receptor-4 (extracellular) antibody, (1:200). 3, 4. Anti-Protease-Activated Receptor-4 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

PAR4 / F2RL3 antibody

  Rabbit polyclonal PAR4 (Cleaved-Gly48) antibody  
Western blot analysis of extracts from 3T3 cells, treated with etoposide (etoposide, 1hour), using PAR4 (Cleaved-Gly48) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAR4 / F2RL3 antibody

  Rabbit polyclonal anti-PAR4 antibody  
Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using PAR4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAR4 / F2RL3 antibody

  Rabbit Polyclonal Anti-F2RL3 Antibody  
Host: Rabbit; Target Name: F2RL3; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAR4 / F2RL3 (2nd cytopl. dom.) antibody

  Rabbit Polyclonal Anti-F2RL3 Antibody (Cytoplasmic Domain)  
Anti-F2RL3 / PAR4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bov, Bt, Eq, Hu, Mky, Ms, Por P
50 µg / €400.00
  OriGene Technologies, Inc.

PAR4 / F2RL3 (2nd extracell. dom.) antibody

  Rabbit Polyclonal Anti-F2RL3 Antibody (Extracellular Domain)  
Anti-F2RL3 / PAR4 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Can, Hu, Ms P
50 µg / €400.00
  OriGene Technologies, Inc.

PAR4 / F2RL3 (C-term) antibody

  Rabbit Polyclonal Anti-F2RL3 Antibody (C-Terminus)  
Anti-F2RL3 / PAR4 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Mky P
50 µg / €400.00
  OriGene Technologies, Inc.

PAR4 / F2RL3 antibody

Immunofluorescence analysis of LOVO cells, using PAR4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

PAR4 / F2RL3 antibody

Immunofluorescence analysis of LOVO cells, using PAR4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

PAR4 / F2RL3 (cleaved) antibody

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using PAR4 (Cleaved-Gly48) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Assay Biotech

PAR4 / F2RL3 (cleaved) antibody

Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using PAR4 (Cleaved-Gly48) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

F2RL3 - human gene knockout kit via CRISPR

F2RL3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

F2RL3 - human gene knockout kit via CRISPR

F2RL3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

F2RL3 - human gene knockout kit via CRISPR

F2RL3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

F2RL3 - human gene knockout kit via CRISPR

F2RL3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PAR4 / F2RL3

  Recombinant protein of human coagulation factor II (thrombin) receptor-like 3 (F2RL3)  
PAR4 / F2RL3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

F2RL3 overexpression lysate

F2RL3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, F2rl3 (GFP-tagged ORF) - Rat coagulation factor II (thrombin) receptor-like 3 (F2rl3), 200 uL, >10^7 TU/mL

Lenti ORF particles, F2rl3 (GFP-tagged ORF) - Rat coagulation factor II (thrombin) receptor-like 3 (F2rl3), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, F2rl3 (Myc-DDK-tagged ORF) - Rat coagulation factor II (thrombin) receptor-like 3 (F2rl3), 200 uL, >10^7 TU/mL

Lenti ORF particles, F2rl3 (Myc-DDK-tagged ORF) - Rat coagulation factor II (thrombin) receptor-like 3 (F2rl3), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

20 Item(s)

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