PARP1 antibody

Principal name

PARP1 antibody

Alternative names for PARP1 antibody

PARP-1, ADPRT, ADPRT 1, PPOL, Poly [ADP-ribose] polymerase 1, NAD(+) ADP-ribosyltransferase 1, Poly[ADP-ribose] synthetase 1

SwissProt ID

P09874 (Human), P11103 (Mouse), P18493 (Bovin), P26446 (Chick), P27008 (Rat), P31669 (Xenla)

Gene ID

142 (PARP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Dros (Drosophila), Hst (Hamster), Xen (Xenopus), Bov (Bovine), Can (Canine), Chk (Chicken), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of PARP1 antibody

PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular “nick sensor” and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.

General readings

Tewari M et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81:801-9 (1995). PubMed PMID: 7774019
Lamarre D et al. Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study. Biochim Biophys Acta 950:147-60 (1988). PubMed PMID: 2454668

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PARP1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PARP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804938) Mouse IgG1 OTI5F1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PARP1 antibody

Human tonsil stained with Anti-PARP antibody Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €440.00
  Acris Antibodies GmbH

PARP1 antibody

Human tonsil stained with Anti-PARP antibody Rabbit Purified Hu, Ms, Rt P, WB

Regular Price: 0.2 ml / €200.00

Special Price: 0.2 ml / €140.00

  Acris Antibodies GmbH

PARP1 antibody

Western blot analysis of extracts from 3T3 cells, treated with etoposide (25uM, 1hour), using PARP (Cleaved-Gly215) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Western blot analysis using PARP mouse mAb against Jurkat (1), K562 (2), HeLa (3), Raji (4), THP-1 (5) and SW620 (6) cell lysate. Mouse 7A10 Purified Hu F, WB
0.1 ml / €345.00
  OriGene Technologies, Inc.

PARP1 antibody

Predicted band size: 113 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 second) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Predicted band size: 113 kDa . Positive control: Hela, K562 and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: K562 cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 second) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Predicted band size: 113 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Western blot analysis of extracts from A549 cells,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Western blot analysis of extracts from A549 cells, treated with etoposide (25uM, 24hours), using PARP (Cleaved-Asp214) antibody. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PARP antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG1 5A5 Purified Hu, Ms ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Western blot analysis of extracts of Jurkat cell line, using PARP1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Western Blot: PARP Antibody (194C1439) [TA336426] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, using this antibody at 2 ug/ml. The band corresponding to cleaved PARP is only seen in the treated samples. anti-mou Mouse IgG2b 194C1439 Purified Hu F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PARP1 antibody

Western blot analysis using PARP antibody Cat.-No AM06413SU-N against Jurkat (Lane 1), K562 (Lane 2), Hela (Lane 3), Raji (Lane 4),THP-1 (Lane 5) and SW620 (Lane 6) cell lysate. Mouse IgG1 7A10 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

PARP1 antibody

Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PARP antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Mouse IgG1 5A5 Purified Hu, Ms ICC/IF, WB
25 µg / €210.00
  Acris Antibodies GmbH

PARP1 antibody

PARP was detected in immersion fixed paraffinembedded sections of human breast using Mouse Anti-Human PARP Monoclonal Antibody at 15 µg/mL overnight at 4°C. Mouse IgG1 839120 Purified Hu P, WB
0.1 ml / €460.00
  Acris Antibodies GmbH

PARP1 Control Peptide

PARP1 Purified
50 µg / €190.00
  Acris Antibodies GmbH

PARP1 (full length, cleaved) antibody

PARP1 Rabbit Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PARP1 (cleaved) antibody

PARP1 Rabbit Aff - Purified Hu P, WB
0.1 mg / €500.00
  Acris Antibodies GmbH

PARP1 (cleaved) antibody

PARP1 Rabbit Aff - Purified Ms ICC/IF, P, WB
0.1 mg / €500.00
  Acris Antibodies GmbH

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