PAX4 antibody

Principal name

PAX4 antibody

Alternative names for PAX4 antibody

Pax-4, Paired box protein Pax-4

SwissProt ID

O43316 (Human), O43316-4, O88436 (Rat), P32115 (Mouse)

Gene ID

5078 (PAX4)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of PAX4 antibody

Pax proteins are a family of transcription factors with a highly conserved paired domain; many members also contain a paired-type homeodomain and/or an octapeptide. Nine mammalian PAX genes are known and classified into four subgroups: Pax-1/9, Pax-2/5/8, Pax-3/7, and Pax-4/6. Most of these genes are involved in nervous system development. The homeo box, which encodes the DNA-binding homeo domain, is a DNA binding sequence motif present in several Drosophila melanogaster developmental genes; it has been used to identify many homologous genes involved in mammalian development. The paired box is another conserved sequence motif, first identified in the paired (prd) and gooseberry (gsb) Drosophila homeo domain genes. The amino-terminal region of the paired domain, including one of the three predicted alpha-helices, is necessary and sufficient for binding. Single amino acid substitution mutation in PAX1 causes formation of the mouse skeletal mutant undulated (un) mutant (1).
PAX4 and PAX6 are highly related and belong to a PAX family of transcription factor that contain both a paired domain and a homeodomain which are potential DNA biding domains. The PAX4 and PAX6 mRNA is expressed at murine embryonic age e9.5 in ventral spinal chord and pancreas. age is expressed in the Pax-4 is necessary for normal development of Pancreas, PAX4 is identified as only regulator of endocrine development of pancreas and its targets genes are not known (2). PAX4 heterozygotes containing a single mutated PAX4 gene are normal however, double null mutant of PAX4 and PAX6 fail to produce any mature pancreatic endocrine cells, suggesting the PAX4 and PAX6 together are required for pancreatic endocrine cell differentiation (2). PAX4 and PAX 6 bind to various sequences in the rat insulin I, somatostatin and glucagons promoters and acts as repressor for gene expression. The homeodoamin and the carboxy portion of the PAX4 molecule confers the majority of the repressive activity. PAX4 expression peaks early in the pancreatic development and it is not expressed in mature islets cells (2).

General readings

1. Haaland WC, Scaduto DI, Maldonado MR, Mansouri DL, Nalini R, Iyer D, Patel S, Guthikonda A, Hampe CS, Balasubramanyam A, Metzker ML,
A-beta-subtype of ketosis-prone diabetes is not predominantly a monogenic diabetic syndrome. Diabetes care 2009 May 32 (5): 873-7. PMID: 19228875

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAX4 antibody

WB Suggested Anti-PAX4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAX4 antibody

Human COLO205; Host: Rabbit . Target Name: PAX4 . Sample Tissue: COLO205 . Antibody Dilution: 1.0ug/ml. PAX4 is supported by BioGPS gene expression data to be expressed in COLO205.; PAX4 antibody - middle region (ARP32064_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

PAX4 antibody

(0.2ug/ml) staining of Mouse Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAX4 antibody

WB Suggested Anti-PAX4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAX4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAX4 (RC210370, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAX4. Mouse IgG2a OTIF3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAX4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAX4 (RC210370, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAX4. Mouse IgG2b OTI3H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAX4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAX4 (RC210370, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAX4. Mouse IgG1 OTIH4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PAX4 antibody

Western blot analysis using PAX4 antibody Cat.-No AM06567SU-N against HEK293 (1) and PAX4(AA: 105-232)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 3C12 Ascites Hu E, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PAX4 Control Peptide

PAX4
0.1 mg / €250.00
  Acris Antibodies GmbH

PAX4 antibody

PAX4 Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PAX4 (Center) antibody

Western blot analysis of PAX4 antibody (Center) (AP17621PU-N) in CEM cell line lysates (35ug/lane). PAX4 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PAX4 antibody

PAX4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PAX4 antibody

Western Blot analysis of PAX4 expression in transfected 293T cell line (H00005078-T02) by PAX4 MaxPab polyclonal antibody.

Lane 1: PAX4 transfected lysate(37.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PAX4 antibody

Human Stomach; WB Suggested Anti-PAX4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Stomach; PAX4 antibody - N-terminal region (ARP32737_P050) in Human Stomach cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PAX4 antibody

PAX4 Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PAX4 antibody

Immunohistochemistry-Paraffin: PAX4 Antibody [NBP1-81043] - Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

PAX4 antibody

Immunohistochemistry-Paraffin: PAX4 Antibody [NBP1-81044] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Western Blot/IP/ChiP Kits

Catalog No. Host Iso. Clone Pres. React. Applications  

PAX4 Western Blot kit

PAX4 Western Blot kit
1 kit / €640.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PAX4 (full length, N-term HIS tag)

PAX4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

PAX4

PAX4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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