PAX8 antibody

Principal name

PAX8 antibody

Alternative names for PAX8 antibody

Pax-8, Paired box protein Pax-8

SwissProt ID

P47240 (Canfa), P51974 (Rat), Q00288 (Mouse), Q06710 (Human), Q9PUK5 (Xenla)

Gene ID

7849 (PAX8), 4747 (NEFL), 18510 (Pax8), 7846 (TUBA1A)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Can (Canine), Rb (Rabbit), Dros (Drosophila), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ChIP assay (ChIP), Frozen Sections (C)

Background of PAX8 antibody

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas.Tissue specificity: Expressed in the excretory system, thyroid gland and Wilms tumors.ABCAM: This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells.

General readings

Castro P, Rebocho AP, Soares RJ, Magalhaes J, Roque L, Trovisco V, Vieira de Castro I, Cardoso-de-Oliveira M, Fonseca E, Soares P, Sobrinho-Simoes M.
PAX8-PPAR{gamma} rearrangement is frequently detected in the follicular variant of papillary thyroid carcinoma.
J Clin Endocrinol Metab. 2005 Oct 11; [Epub ahead of print]
PMID: 16219715

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PAX8 antibody

Sample(30 ug whole cell lysate). A:293T. 10% SDS PAGE. TA308698 diluted at 1:1000 Rabbit IgG Aff - Purified Can, Hu, Ms, Rt, Xen ChIP, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAX8 antibody

PAX8 monoclonal antibody (M09), clone 3H7. Western Blot analysis of PAX8 expression in human liver. Mouse IgG2a 3H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAX8 (N-term) antibody

Staining of paraffin embedded Human Thyroid Gland using PAX8 Antibody Cat.-No AP16042PU-N at 4 µg/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PAX8 antibody

TA302560 (0.5µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PAX8 antibody

Predicted band size: 48 kDa . Positive control: MCF7 cell and mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: MCF7 cell lysate Lane2: Mouse brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAX8 antibody

Predicted band size: 48 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PAX8 antibody

Immunohistochemistry staining of Paraffin Ovarian carcinoma (non-mucinous carcinoma) tissue by Pax-8 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear) Mouse IgG MRQ-50 Purified Hu P
1 ml / €490.00
  OriGene Technologies, Inc.

PAX8 antibody

Host: Rabbit; Target Name: PAX8; Sample Tissue: Pancreas Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAX8 antibody

WB Suggested Anti-PAX8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: NCI-H226 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PAX8 antibody

Western blot analysis using PAX8 antibody Cat.-No AM06532SU-N against Hela (Lane 1), HEK293 (Lane 2) and Raji (Lane 3) cell lysate. Mouse IgG1 2D4 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

PAX8 antibody

Formalin-Fixed, Paraffin-Embedded endometrial clear cell carcinoma stained with PAX8 antibody Cat.-No. AM10131SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of tumor cells. Rabbit IgG ZR-1 Supernatant Hu P
1 ml / €610.00
  Acris Antibodies GmbH

PAX8 (N-term, all isoforms) antibody

Immunohistochemical staining of Formalin-fixed, Paraffin embedded human kidney tissue (cortex) using anti-PAX8 antibody cat.no. AP07520PU-N Goat Aff - Purified Can, Elephant, Hu, Mky, Ms, Op, Platypus, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PAX8 (Center) antibody

Western blot analysis of PAX8 Antibody (Center) (AP17623PU-N) in HL-60 cell line lysates (35ug/lane). PAX8 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PAX8 antibody

Immunohistochemistry (IHC) analyzes of Pax-8 antibody (Cat.-No.: AP20584PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PAX8 antibody

PAX8 monoclonal antibody (M03), clone 3B11. Western Blot analysis of PAX8 expression in RIN-m5F. (60kD,Endocrinology Vol. 140, No. 10 4651-4658,http://endo.endojournals.org/cgi/content/full/140/10/4651) Mouse IgG2b 3B11 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAX8 antibody

Western Blot analysis of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M04), clone 2D6.

Lane 1: PAX8 transfected lysate(48.119 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2D6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAX8 antibody

Western Blot analysis of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M05), clone 3E9.

Lane 1: PAX8 transfected lysate(48.2 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 3E9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAX8 antibody

Detection limit for recombinant GST tagged PAX8 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 1C3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAX8 antibody

Western Blot analysis of PAX8 expression in transfected 293T cell line by PAX8 monoclonal antibody (M07), clone 1A6.

Lane 1: PAX8 transfected lysate(48.119 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PAX8 antibody

Detection limit for recombinant GST tagged PAX8 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 3A10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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