Paxillin / PXN antibody

Principal name

Paxillin / PXN antibody

Alternative names for Paxillin / PXN antibody

FLJ16691

SwissProt ID

P49023 (Human), P49024 (Chick), Q66H76 (Rat), Q8VI36 (Mouse)

Gene ID

5829 (PXN), 395832, 19303 (Pxn), 360820

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Bov (Bovine), Eq (Equine), Mky (Monkey), Rb (Rabbit), Xen (Xenopus), Ze (Zebrafish), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of Paxillin / PXN antibody

PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al. (1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein–protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites. On Northern blots, paxillin was expressed as a 3.7-kb mRNA in all tissues tested.

General readings

1 Salgia, R.; Li, J.-L.; Lo, S. H.; Brunkhorst, B.; Kansas, G. S.; Sobhany, E. S.; Sun, Y.; Pisick, E.; Hallek, M.; Ernst, T.; Tantravahi, R.; Chen, L. B.; Griffin, J. D. : Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210(BCR/ABL). J. Biol. Chem. 270: 5039-5047, 1995.
2. Turner, C. E.; Glenney, J. R., Jr.; Burridge, K. : Paxillin: a new vinculin-binding protein present in focal adhesions. J. Cell Biol. 111: 1059-1068, 1990.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Paxillin / PXN pTyr118 antibody

  Rabbit Polyclonal Antibody against PXN (Y118)  
Western blot analysis of PXN(arrow) using rabbit polyclonal PXN Antibody (Y118) (Cat.#TA302184). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit anti-PXN (Paxillin, Phospho-Tyr31) polyclonal antibody(Phospho-specific)  
Western blot analysis of extracts from SK-OV3 cells untreated or treated with estradiol (0.1uM, 20min) using Paxillin antibody (Lanes 1 and 2) and Paxillin (phospho-Tyr31) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Anti-PXN Rabbit Polyclonal Antibody  
Predicted band size: 66 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Anti-PXN Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using PXN antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Anti-PXN (Phospho-Tyr118) Rabbit Polyclonal Antibody  
Predicted band size: 65 kDa . Positive control: NIH/3T3 cells untreated or treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: NIH/3T3 cells untreated with serum lysate Lane2: NIH/3T3 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Anti-PXN (Phospho-Tyr31) Rabbit Polyclonal Antibody  
Predicted band size: 65 kDa . Positive control: HT29 cells treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HT29 cells untreated with PMA lysate Lane2: HT29 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit polyclonal Paxillin (Phospho-Ser178) antibody  
Western blot analysis of extracts from HT-29, using Paxillin (Phospho-Ser178) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit polyclonal Paxillin (Ab-272) antibody  
Western blot analysis of extracts from HeLa cells, using Paxillin (Ab-272) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit polyclonal Paxillin (Phospho-Ser272) antibody  
Western blot analysis of extracts from HeLa and JK, using Paxillin (Phospho-Ser272) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit Polyclonal Phospho-Paxillin (Tyr118) Antibody (Phospho-specific)  
Western blot analysis of Paxillin phosphorylation expression in EGF treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit Polyclonal Phospho-Paxillin (Tyr31) Antibody (Phospho-specific)  
Western blot analysis of Paxillin phosphorylation expression in Estradiol treated SK-OV3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit Polyclonal Paxillin Antibody  
Western blot analysis of Paxillin expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit Polyclonal Paxillin Antibody  
Western blot analysis of Paxillin expression in Estradiol treated SK-OV3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Phospho-PXN-Y118 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from 3T3 cells, using phospho-PXN-Y118 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

  Rabbit Polyclonal Anti-Phospho-Paxillin(Ser178) Antibody  
Western blot analysis on HeLa cell lysate using Phospho-Paxillin(Ser178) Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN (C-term) antibody

Paxillin /  PXN Mouse IgG1 PAX-14 Purified Chk, Hu, Ms, Rt ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Paxillin / PXN antibody

Immunofluorescent microscope analysis of Hela cells using Paxillin / PXN Antibody (clone PAX227) Cat.-No AM33052PU-S, followed by Rhodamine Red-X conjugated Donkey anti-Mouse IgG and DAPI. Mouse IgG1 PAX227 Purified Hu, Ms ICC/IF, IP, P, WB
50 µl / €190.00
  Acris Antibodies GmbH

Paxillin / PXN (C-term) antibody

Paxillin /  PXN Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Paxillin / PXN pTyr118 antibody

Western blot (WB) analysis of Paxillin antibody (pTyr118, Cat.-No.: AP01665PU-N) in extracts from NMµMG cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Paxillin / PXN pTyr118 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (phospho-Tyr118) antibody (AP02387PU). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

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