Paxillin / PXN antibody

Principal name

Paxillin / PXN antibody

Alternative names for Paxillin / PXN antibody

FLJ16691

SwissProt ID

P49023 (Human), P49024 (Chick), Q66H76 (Rat), Q8VI36 (Mouse)

Gene ID

5829 (PXN), 395832, 19303 (Pxn), 360820

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Hst (Hamster), Chimp (Chimpanzee), Mky (Monkey), Chk (Chicken), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Paxillin / PXN antibody

Cellular adhesion to the extracellular matrix is critically involved in many processes including normal and transformed cell growth, migration, metastasis, lymphocyte extravasation and also in force transmission during muscle contraction. Paxillin (68 kDa), is a cytoskeletal component found in the focal adhesions at the ends of actin stress fibers, but not in adherens junctions of the cells. Paxillin interacts with several proteins including members of the src family of tyrosine kinases, the transforming protein v-crk, the cytoskeletal protein vinculin, and the tyrosine kinase, focal adhesion kinase (FAK). This interaction has suggested a function for paxillin as a molecular adaptor, responsible for the recruitment of structural and signaling molecules to focal adhesions. The paxillin molecule has a single binding site for vinculin, and at least two binding sites for FAK, that are separated by an intervening sequence of 100 amino acids. Upon transformation by RSV, 20-30% of the paxillin molecules are phosphorylated on tyrosine. In addition, the cellular distribution of paxillin becomes more diffuse, suggesting that phosphorylation of paxillin may have a role in the disassembly of focal adhesions and stress fibers during transformation. The availability of monoclonal antibody reacting specifically with paxillin enables the subcellular detection and localization of paxillin.

General readings

1 Salgia, R.; Li, J.-L.; Lo, S. H.; Brunkhorst, B.; Kansas, G. S.; Sobhany, E. S.; Sun, Y.; Pisick, E.; Hallek, M.; Ernst, T.; Tantravahi, R.; Chen, L. B.; Griffin, J. D. : Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210(BCR/ABL). J. Biol. Chem. 270: 5039-5047, 1995.
2. Turner, C. E.; Glenney, J. R., Jr.; Burridge, K. : Paxillin: a new vinculin-binding protein present in focal adhesions. J. Cell Biol. 111: 1059-1068, 1990.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Paxillin / PXN antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PXN and PTEN Mahlavu cells were stained with anti-PXN rabbit purified polyclonal 1:1200 and anti-PTEN mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Paxillin / PXN pTyr118 antibody

Western blot analysis of PXN(arrow) using rabbit polyclonal PXN Antibody (Y118) (Cat.#TA302184). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with PXN gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

Predicted band size: 66 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using PXN antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

Western blot analysis of extracts from HeLa cells, using Paxillin (Ab-272) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

Western blot analysis of Paxillin expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN antibody

Western blot analysis of Paxillin expression in Estradiol treated SK-OV3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Paxillin / PXN (C-term) antibody

Paxillin /  PXN Mouse IgG1 PAX-14 Purified Chk, Hu, Ms, Rt ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Paxillin / PXN antibody

Immunofluorescent microscope analysis of Hela cells using Paxillin / PXN Antibody (clone PAX227) Cat.-No AM33052PU-S, followed by Rhodamine Red-X conjugated Donkey anti-Mouse IgG and DAPI. Mouse IgG1 PAX227 Purified Hu, Ms ICC/IF, IP, P, WB
50 µl / €190.00
  Acris Antibodies GmbH

Paxillin / PXN (C-term) antibody

Paxillin /  PXN Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Paxillin / PXN pTyr118 antibody

Western blot (WB) analysis of Paxillin antibody (pTyr118, Cat.-No.: AP01665PU-N) in extracts from NMµMG cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Paxillin / PXN pTyr118 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (phospho-Tyr118) antibody (AP02387PU). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Paxillin / PXN pTyr118 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (phospho-Tyr118) antibody (AP02387PU). Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

Paxillin / PXN pTyr31 antibody

Western blot analysis of extracts from SK-OV3 cells untreated or estradiol-treated (0.1μM, 20min), using Paxillin (pTyr31) antibody (AP02469PU-N, Lane 3 and 4). Rabbit Aff - Purified Hu WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Paxillin / PXN pTyr31 antibody

Western blot analysis of extracts from SK-OV3 cells untreated or estradiol-treated (0.1μM, 20min), using Paxillin (pTyr31) antibody (AP02469PU-N, Lane 3 and 4). Rabbit Aff - Purified Hu WB
50 µl / €240.00
  Acris Antibodies GmbH

Paxillin / PXN antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin antibody (AP02638PU). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Paxillin / PXN antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin antibody (AP02638PU). Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Paxillin / PXN antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin antibody AP02711PU. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Paxillin / PXN antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin antibody AP02711PU. Rabbit Aff - Purified Hu P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Paxillin / PXN antibody

Immunohistochemistry (IHC) analyzes of Paxillin antibody (Cat.-No.: AP06280PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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