PCAF / KAT2B antibody

Principal name

PCAF / KAT2B antibody

Alternative names for PCAF / KAT2B antibody

Histone acetylase PCAF, P/CAF, Histone acetyltransferase PCAF, Histone acetyltransferase KAT2B, Lysine acetyltransferase 2B, P300/CBP-associated factor

SwissProt ID

Q92831 (Human), Q9JHD1 (Mouse)

Gene ID

8850 (KAT2B), 18519 (Kat2b)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of PCAF / KAT2B antibody

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. PCAF associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptiol regulation.CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptiol regulation.CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptiol regulation. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordites used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-749 AC099057.3 17115-17863 750-876 AC099057.3 49416-49542 877-1022 AC099057.3 72346-72491 1023-1115 AC099057.3 76945-77037 1116-1297 AC099057.3 78370-78551 1298-1489 AC099057.3 88679-88870 1490-1596 AC099057.3 91965-92071 1597-1722 AC099057.3 96681-96806 1723-1859 AC099057.3 99751-99887 1860-2068 AC099057.3 102988-103196 2069-2195 AC099057.3 104506-104632 2196-2306 AC099057.3 114025-114135 2307-2450 AC099057.3 117304-117447 2451-2565 AC099057.3 123399-123513 2566-2602 AC099057.3 125046-125082 2603-2666 AC099057.3 125326-125389 2667-2751 AC099057.3 125486-125570 2752-4824 AC099057.3 129415-131487

General readings

Chu,B.Y., Biochem. J. 392 (PT 3), 589-599 (2005)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCAF / KAT2B antibody

  Rabbit Polyclonal antibody to PCAF (K(lysine) acetyltransferase 2B)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308325 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PCAF / KAT2B antibody

  Rabbit Polyclonal p300/CBP Antibody  
Western blot analysis on COLO205 cell lysate using PCAF Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PCAF / KAT2B antibody

  Rabbit Polyclonal Anti-PCAF Antibody  
WB Suggested Anti-PCAF Antibody Titration: 2.5ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCAF / KAT2B antibody

  Rabbit Polyclonal Anti-PCAF Antibody - C-terminal region  
PCAF / KAT2B Rabbit IgG Purified Hu, Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

PCAF / KAT2B antibody

  Mouse Monoclonal anti-Acetylated Lysine Antibody  
Untreated  Hela cells  (lane 1) and sodium butyrate-treated Hela cells (24 hr treatment, lane 2) were lysed and cell extracts resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetylated lysine antibody (clone 15G10). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Sodium butyrate-treated cells show a variety of acetylated proteins with molecular weights consistent with histones, p53, CBP/p300, and PCAF (among others). Mouse 15G10 Purified ICC/IF, WB
25 µg / €248.00
  OriGene Technologies, Inc.

PCAF / KAT2B antibody

  Rabbit Polyclonal PCAF Antibody  
ELISA: PCAF Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCAF / KAT2B antibody

Immunohistochemistry (IHC) analyzes of PCAF antibody (Cat.-No.: AP06405PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PCAF / KAT2B (C-term) antibody

PCAF Antibody (C-term) (Cat. #AP53187PU-N) western blot analysis in U251 cell line lysates (35µg/lane).This demonstrates the PCAF antibody detected the PCAF protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PCAF / KAT2B (N-term) antibody

PCAF Antibody (N-term) (Cat. #AP53188PU-N) western blot analysis in MDA-MB231 cell line lysates (35µg/lane).This demonstrates the PCAF antibody detected the PCAF protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PCAF / KAT2B antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using p300/CBP Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

PCAF / KAT2B antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using p300/CBP Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

KAT2B - human gene knockout kit via CRISPR

KAT2B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

KAT2B - human gene knockout kit via CRISPR

KAT2B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

KAT2B - human gene knockout kit via CRISPR

KAT2B - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

KAT2B - human gene knockout kit via CRISPR

KAT2B - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Kat2b - mouse gene knockout kit via CRISPR

Kat2b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Kat2b - mouse gene knockout kit via CRISPR

Kat2b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Kat2b - mouse gene knockout kit via CRISPR

Kat2b - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Kat2b - mouse gene knockout kit via CRISPR

Kat2b - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PCAF / KAT2B

  Recombinant protein of human K(lysine) acetyltransferase 2B (KAT2B)  
PCAF / KAT2B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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