PCBP1 antibody

Principal name

PCBP1 antibody

Alternative names for PCBP1 antibody

hnRNP E1, HNRPE1, HNRPX, Alpha-CP1, SUB2.3, Poly(rC)-binding protein 1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3

SwissProt ID

O19048 (Rabit), P60335 (Mouse), Q15365 (Human), Q5E9A3 (Bovin)

Gene ID

5093 (PCBP1)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rb (Rabbit), Rt (Rat), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PCBP1 antibody

PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5\'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.This intronless gene is thought to be generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5\'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5\'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Waggoner SA, Johannes GJ, Liebhaber SA,
Depletion of the poly(C)-binding proteins alphaCP1 and alphaCP2 from K562 cells leads to p53-independent induction of cyclin-dependent kinase inhibitor (CDKN1A) and G1 arrest.
The Journal of biological chemistry 2009 Apr 284 (14): 9039-49. PMID: 19211566

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCBP1 antibody

Host: Rabbit; Target Name: PCBP1; Sample Tissue: MCF7 Whole Cell lysates; Antibody Dilution: 1.0 ug/mlPCBP1 is supported by BioGPS gene expression data to be expressed in MCF7 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PCBP1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806353) Mouse IgG1 OTI3D12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCBP1 antibody

Human spleen; Anti-PCBP1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.; PCBP1 Antibody - middle region (ARP40631_P050) in Human spleen cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

PCBP1 (223-234) antibody

(0.1ug/ml) staining of PBMC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCBP1 (234-247) antibody

(0.01ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCBP1 antibody

WB Suggested Anti-PCBP1 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysatePCBP1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCBP1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PCBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806249) Mouse IgG2b OTI3B9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCBP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCBP1 (RC207878, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCBP1. Mouse IgG1 OTI10B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCBP1 antibody

The lysate of Hela (40ug) and HepG2(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT2A10 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PCBP1 antibody

The lysate of Hela (40ug) and HepG2(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT2A10 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

PCBP1 antibody

PCBP1 polyclonal antibody (A01), Lot # 050928JC01 Western Blot analysis of PCBP1 expression in Y-79 ( Cat # L042V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PCBP1 antibody

PCBP1 Mouse IgG2a 1G2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PCBP1 antibody

PCBP1 Mouse IgG2a 1G2 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

PCBP1 antibody

HepG2; Host: Rabbit. Target Name:PCBP1. Sample Tissue:HepG2. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:2.5ug/mL. Peptide Concentration: 2.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%. PCBP1 is supported by BioGPS gene expression data to be expressed in HepG2.; PCBP1 antibody - middle region (ARP40630_T100) in HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

PCBP1 antibody

Western blot analysis of hnRNP E1 expression in HeLa (A); K562 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PCBP1 antibody

Western blot analysis of hnRNP E1 expression in HeLa (A); K562 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PCBP1 antibody

Western blot analysis of hnRNP E1 expression in HeLa (A); K562 (B); SP2/0 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

PCBP1 antibody

PCBP1 Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PCBP1 antibody

PCBP1 Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PCBP1 antibody

PCBP1 Rabbit Purified Bov, Hu, Ms, Rb, Rt WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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