PCK1 / PEPCK1 antibody

Principal name

PCK1 / PEPCK1 antibody

Alternative names for PCK1 / PEPCK1 antibody

PEPCK-C, PEPCKC, Phosphoenolpyruvate carboxylase, PEP carboxykinase, Phosphoenolpyruvate carboxykinase cytosolic [GTP]

SwissProt ID

P05153 (Chick), P07379 (Rat), P35558 (Human), Q8HYZ4 (Bovin), Q9Z2V4 (Mouse)

Gene ID

5105 (PCK1)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Pl (Plant), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C)

Background of PCK1 / PEPCK1 antibody

PCK1 is a main control point for the regulation of gluconeogenesis. PCK1, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human PCK1 with an internal ID of P03819

General readings

Willer CJ, Bonnycastle LL, Conneely KN, Duren WL, Jackson AU, Scott LJ, Narisu N, Chines PS, Skol A, Stringham HM, Petrie J, Erdos MR, Swift AJ, Enloe ST, Sprau AG, Smith E, Tong M, Doheny KF, Pugh EW, Watanabe RM, Buchanan TA, Valle TT, Bergman RN, Tuomilehto J, Mohlke KL, Collins FS, Boehnke M.
Screening of 134 single nucleotide polymorphisms (SNPs) previously associated with type 2 diabetes replicates association with 12 SNPs in nine genes.
Diabetes. 2007 Jan;56(1):256-64.
PMID: 17192490

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCK1 / PEPCK1 antibody

Western blot analysis of extracts of various cell lines, using PCK1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCK1 / PEPCK1 antibody

WB Suggested Anti-PCK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PCK1 / PEPCK1 antibody

WB Suggested Anti-PCK1 Antibody Titration: 1.0ug/ml; Positive Control: Fetal Brain Lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCK1 / PEPCK1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679) Rabbit IgG Purified Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCK1 / PEPCK1 antibody

Hum. Fetal Heart; Host: Rabbit. Target Name: PCK1. Sample Tissue: Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; PCK1 antibody - middle region (ARP32801_P050) in Hum. Fetal Heart cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PCK1 / PEPCK1 antibody

Human kidney; PCK1 antibody - middle region (ARP32801_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

PCK1 / PEPCK1 antibody

TA303224 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCK1 / PEPCK1 antibody

TA305772 (2ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Can, Hu, Ms, Rt E, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCK1 / PEPCK1 antibody

Western blot analysis of PEPCK-C with lysate from rat kidney (Lane 1), 3T3 cells (Lane 2) and Jurkat cells (Lane 3). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCK1 / PEPCK1 antibody

WB Suggested Anti-PCK1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PCK1 / PEPCK1 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PCK1 / PEPCK1 (513-524) antibody

Human Liver (formalin-fixed, paraffin-embedded) stained with PCK1 antibody AP22519PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Insect, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr, Xen E, P
50 µg / €470.00
  Acris Antibodies GmbH

PCK1 / PEPCK1 antibody

PCK1 MaxPab polyclonal antibody. Western Blot analysis of PCK1 expression in human kidney. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PCK1 / PEPCK1 antibody

PCK1 / PEPCK1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PCK1 / PEPCK1 antibody

PCK1 / PEPCK1 Rabbit Serum Hu IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PCK1 / PEPCK1 antibody

Western Blot analysis of PCK1 expression in transfected 293T cell line (H00005105-T02) by PCK1 MaxPab polyclonal antibody.

Lane 1: PCK1 transfected lysate(69.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PCK1 / PEPCK1 antibody

Immunofluorescence of monoclonal antibody to PCK1 on HepG2 cell. [antibody concentration 10 ug/ml] Mouse IgG1 3E4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PCK1 / PEPCK1 antibody

Detection limit for recombinant GST tagged PCK1 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 3B12 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PCK1 / PEPCK1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PCK1 polyclonal antibody ( Cat # PAB3309 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PCK1 / PEPCK1 antibody

PCK1 polyclonal antibody ( Cat # PAB6874 ) ( 2µg/ml ) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu E, P
0.1 mg / €400.00
  Abnova Taiwan Corp.

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