PCLO antibody

Principal name

PCLO antibody

Alternative names for PCLO antibody

Protein piccolo, Aczonin, ACZ, KIAA0559

SwissProt ID

Q9JKS6 (Rat), Q9PU36 (Chick), Q9QYX7 (Mouse), Q9Y6V0 (Human)

Gene ID

27445 (PCLO), 56768 (Pclo)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of PCLO antibody

Piccolo is a presynaptic cytomatrix protein concentrated at the presynaptic side of synaptic junctions. Piccolo is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. It is usually found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1. Piccolo is highly expressed in brain with low levels found in stomach. It is not detected in other tissues analyzed including adrenal gland, testis and pancreas.

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCLO antibody

Western blot analysis of Piccolo in mouse and rat brain lysate, using a 1:1000 dilution of the antibody Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCLO antibody

WB analysis of PCLO using the antibody. Mouse IgG2b 6H9-B6 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCLO antibody

Host: Rabbit; Target Name: PCLO; Sample Tissue: Hela Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PCLO antibody

Western blot of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by  mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting. Mouse IgG1 PCLO-01 Aff - Purified Hu F, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

PCLO antibody

Western blot of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by  mAb PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting. Mouse IgG1 PCLO-01 PE Hu F
0.1 mg / €290.00
  Acris Antibodies GmbH

PCLO antibody

Western Blot analysis of PCLO expression in transfected 293T cell line (H00027445-T01) by PCLO MaxPab polyclonal antibody.

Lane 1: PCLO transfected lysate(39.16 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PCLO antibody

Immunofluorescent: Piccolo Antibody [NBP1-03406] - IF on 1 day-old mouse cortical cell culture using Rabbit antibody to internal part of Piccolo (Aczonin): whole serum (NBP1-03406) at 1: 2000 dilution. DAPI counter stained. Rabbit Serum Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

PCLO antibody

PCLO Mouse 6H9-B6 Purified Hu, Rt E, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

PCLO antibody

PCLO Mouse 6H9-B6 Purified Hu, Rt E, WB
25 µg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PCLO

PCLO Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PCLO

PCLO Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PCLO 293T Cell Transient Overexpression Lysate(Denatured)

PCLO 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

12 Item(s)

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