PCM1 antibody

Principal name

PCM1 antibody

Alternative names for PCM1 antibody

PCM-1, Pericentriolar material 1 protein

SwissProt ID

Q15154 (Human), Q8AV28 (Chick), Q923L0 (Mouse), Q9PVV4 (Xenla), Q9R0L6 (Mouse)

Gene ID

5108 (PCM1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of PCM1 antibody

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCM1 antibody

Western blot analysis of PCM-1 antibody (Center)(Cat.#TA324939) in Ramos cell line lysates (35ug/lane). PCM-1 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

PCM1 antibody

Western blot analysis of extracts of various cell lines, using PCM1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCM1 antibody

Western Blot analysis of PCM1 expression in transfected 293T cell line (H00005108-T01) by PCM1 MaxPab polyclonal antibody.

Lane 1: PCM1 transfected lysate(58.3 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PCM1 antibody

Western Blot and Immunoprecipitation: PCM1 Antibody [NB100-58828] - Detection of Human and Mouse PCM1 on HeLa whole cell lysate using NB100-58828. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58829. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PCM1 antibody

Western Blot and Immunoprecipitation: PCM1 Antibody [NB100-58829] - Detection of Human PCM1 on HeLa whole cell lysate using NB100-58829. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58828. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PCM1 antibody

PCM1 Rabbit Purified Hu, Ms C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

PCM1 antibody

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87195] - Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

PCM1 antibody

Immunohistochemistry-Paraffin: PCM1 Antibody [NBP1-87196] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PCM1

PCM1 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PCM1 293T Cell Transient Overexpression Lysate(Denatured)

PCM1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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