PCNA antibody

Principal name

PCNA antibody

Alternative names for PCNA antibody

Cyclin, Proliferating Cell Nuclear Antigen

SwissProt ID

O22012 (Isoga), P04961 (Rat), P12004 (Human), P15873 (Yeast), P17918 (Mouse), P18248 (Xenla), Q3ZBW4 (Bovin)

Gene ID

5111 (PCNA), 25737 (Pcna), 18538

Available reactivities

Amph (Amphibian), Dros (Drosophila), Fi (Fish), Mam (Mammalia), Ye (Yeast), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Prim (Primate), Insect, Chk (Chicken), Mky (Monkey), Hst (Hamster), Por (Porcine), Rb (Rabbit), Xen (Xenopus), S. cerevisiae (Saccharomyces cerevisiae)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of PCNA antibody

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle It is increased during late G1 phase and S phase of the cell cycle and declines during G2 and M phases.

General readings

1. Chuang, L.S.-H., Ian, H.-I., Koh, T.-W., Ng, H.-H., Xu, G., Li, B.F.L. (1997) Human DNA-(cytosine-5) methyltransferase-PCNA complex as a target for p21WAF1. Science 277:1996-2000.
2. Dzantiev, L., Constantin, N., Genschel, J., Iyer, R.R., Burgers, P.M., Modrich, P. (2004)
A defined human system that supports bidirectional mismatch-provoked excision. Mol. Cell 15:31-41.
3. Haracska, L., Unk I., Johnson, R.E., Phillips, B.B., Hurwitz, J., Prakash, L., Prakash, S. (2002) Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA. Mol. Cell. Biol. 22:784-791.
4. Kannouche, P.L., Wing J., Lehmann, A.R. (2004) Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage. Mol. Cell 14:491-500.
5. Travali, S., Ku, D.H., Rizzo, M.G., Ottavio, L., Baserga, R., Calabretta, B. (1989) Structure of the human gene for the proliferating cell nuclear antigen. J. Biol. Chem. 264:7466-7472.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCNA antibody

  Rabbit polyclonal anti-PCNA antibody, Loading control  
Western blot analysis of extracts of various cell lines, using PCNA antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCNA antibody

  Rabbit Polyclonal PCNA Antibody  
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCNA antibody

  Mouse monoclonal anti-PCNA antibody, clone 2G7, Loadi, clone OTI2G7 (formerly 2G7)  
Immunofluorescent staining of HeLa cells using anti-PCNA mouse monoclonal antibody (TA800875, green, 1:50). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue). Mouse IgG2b OTI2G7 Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI4G3 (formerly 4G3)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800876) Mouse IgG1 OTI4G3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  Mouse monoclonal anti-PCNA antibody, clone 4B2, Loadi, clone OTI4B2 (formerly 4B2)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800880) Mouse IgG2b OTI4B2 Purified Hu P, WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI3D6 (formerly 3D6)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800894) Mouse IgG2a OTI3D6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI1H10 (formerly 1H10)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800899) Mouse IgG2b OTI1H10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI3F11 (formerly 3F11)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800915) Mouse IgG2a OTI3F11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI6A10 (formerly 6A10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800975) Mouse IgG1 OTI6A10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI6B6 (formerly 6B6)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800976) Mouse IgG2a OTI6B6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI8C12 (formerly 8C12)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800983) Mouse IgG2b OTI8C12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI1D11 (formerly 1D11)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800985) Mouse IgG2a OTI1D11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI13G4 (formerly 13G4)  
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804935) Mouse IgG2a OTI13G4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

  PCNA mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804965) Mouse IgG2b OTI1A5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Formalin-paraffin human tonsil stained with PCNA Antibody Cat.-No AM50151PU (Clone SPM350) Mouse IgG2a SPM350 Purified Chk, Dros, Hu, Ms, Rt, Ye, Ze C, F, ICC/IF, IP, P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

PCNA antibody

Formalin-paraffin human tonsil stained with PCNA Antibody Cat.-No AM50151PU (Clone SPM350) Mouse IgG2a SPM350 Purified Chk, Dros, Hu, Ms, Rt, Ye, Ze C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

PCNA antibody

Formalin-paraffin human tonsil stained with PCNA Antibody Cat.-No AM50151PU (Clone SPM350) Mouse IgG2a SPM350 Purified Chk, Dros, Hu, Ms, Rt, Ye, Ze C, F, ICC/IF, IP, P, WB
20 µg / €180.00
  Acris Antibodies GmbH

PCNA (111-112) antibody

Staining of Pig Brain lysate using PCNA Antibody Cat.-No AP32026PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PCNA antibody

  Mouse Monoclonal PCNA Antibody (PC10)  
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG2a PC10 Purified Chk, Dros, Hu, Ms, Rt, Ye C, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PCNA antibody

  Rabbit polyclonal anti-PCNA antibody  
Western blot analysis of extracts from HepG2 cells, using PCNA antibody (#TA312025).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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