PCNA antibody

Principal name

PCNA antibody

Alternative names for PCNA antibody

Cyclin, Proliferating Cell Nuclear Antigen

SwissProt ID

O22012 (Isoga), P04961 (Rat), P12004 (Human), P15873 (Yeast), P17918 (Mouse), P18248 (Xenla), Q3ZBW4 (Bovin)

Gene ID

5111 (PCNA), 25737 (Pcna)

Available reactivities

Hu (Human), Insect, Ms (Mouse), Rt (Rat), Ye (Yeast), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Mam (Mammalia), Prim (Primate), Chk (Chicken), Dros (Drosophila), Mky (Monkey), Fi (Fish), Xen (Xenopus), Hst (Hamster), Amph (Amphibian), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (detection) (E(detection)), PLA/Proximity ligation assay (PLA), Luminex (LMNX)

Background of PCNA antibody

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

General readings

1. Chuang, L.S.-H., Ian, H.-I., Koh, T.-W., Ng, H.-H., Xu, G., Li, B.F.L. (1997) Human DNA-(cytosine-5) methyltransferase-PCNA complex as a target for p21WAF1. Science 277:1996-2000.
2. Dzantiev, L., Constantin, N., Genschel, J., Iyer, R.R., Burgers, P.M., Modrich, P. (2004)
A defined human system that supports bidirectional mismatch-provoked excision. Mol. Cell 15:31-41.
3. Haracska, L., Unk I., Johnson, R.E., Phillips, B.B., Hurwitz, J., Prakash, L., Prakash, S. (2002) Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA. Mol. Cell. Biol. 22:784-791.
4. Kannouche, P.L., Wing J., Lehmann, A.R. (2004) Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage. Mol. Cell 14:491-500.
5. Travali, S., Ku, D.H., Rizzo, M.G., Ottavio, L., Baserga, R., Calabretta, B. (1989) Structure of the human gene for the proliferating cell nuclear antigen. J. Biol. Chem. 264:7466-7472.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCNA antibody

Western blot analysis of extracts of various cell lines, using PCNA antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCNA antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-PCNA Rabbit Polyclonal antibody. (TA590137) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCNA antibody

Immunofluorescent staining of HeLa cells using anti-PCNA mouse monoclonal antibody (TA800875, green, 1:50). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue). Mouse IgG2b OTI2G7 Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800876) Mouse IgG1 OTI4G3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800880) Mouse IgG2b OTI4B2 Purified Hu P, WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800894) Mouse IgG2a OTI3D6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800899) Mouse IgG2b OTI1H10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800915) Mouse IgG2a OTI3F11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800975) Mouse IgG1 OTI6A10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800976) Mouse IgG2a OTI6B6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800983) Mouse IgG2b OTI8C12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800985) Mouse IgG2a OTI1D11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804935) Mouse IgG2a OTI13G4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PCNA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804965) Mouse IgG2b OTI1A5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PCNA antibody

Immunofluorescence of monoclonal antibody to PCNA on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 1G7 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PCNA antibody

Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M04), clone S1.

Lane 1: PCNA transfected lysate(28.8 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b S1 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PCNA antibody

Human NT-2; Data Provided by Dr. Yuzhi Chen, University of Arkansas for Medical SciencesNT2 cells were pretreated with 2N HCL for 30 minutesWashed 3x in PBSStained for 2 hours with 1ug/50ul antibodyand Incubated in Alexa goat-rabbit 594 for 1H.Images were obtained with a 40x objectiveNT-2 cellsAntibody: RedDAPI: Blue; PCNA antibody - C-terminal region (AVARP03018_T100) in Human NT-2 cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

PCNA antibody

Formalin-paraffin human tonsil stained with PCNA Antibody Cat.-No AM50151PU (Clone SPM350) Mouse IgG2a SPM350 Purified Chk, Dros, Hu, Ms, Rt, Ye, Ze C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

PCNA antibody

Formalin-paraffin human tonsil stained with PCNA Antibody Cat.-No AM50151PU (Clone SPM350) Mouse IgG2a SPM350 Purified Chk, Dros, Hu, Ms, Rt, Ye, Ze C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

PCNA antibody

Formalin-paraffin human tonsil stained with PCNA Antibody Cat.-No AM50151PU (Clone SPM350) Mouse IgG2a SPM350 Purified Chk, Dros, Hu, Ms, Rt, Ye, Ze C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

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