PCOLCE antibody

Principal name

PCOLCE antibody

Alternative names for PCOLCE antibody

PCPE1, PCPE-1, Procollagen C-endopeptidase enhancer 1, Procollagen COOH-terminal proteinase enhancer 1, Procollagen C-proteinase enhancer 1, Type 1 procollagen C-proteinase enhancer protein, Type I procollagen COOH-terminal proteinase enhancer

SwissProt ID

O08628 (Rat), Q15113 (Human), Q61398 (Mouse)

Gene ID

5118 (PCOLCE), 18542 (Pcolce)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Rat, Goat

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of PCOLCE antibody

BMP1, also known as Bone Morphogenetic Protein 1, Procollagen C-endopeptidase, Procollagen C-peptidase, or Procollagen C-proteinase, is an extracellular zinc endopeptidase of the astacin family. PCOLE1 was described as an accessory protein that enhances the activity of BMP1. Another BMP1 enhancer protein, PCOLE2, was described in a subtractive library of eye tissue, and little is known about its function. PCOLE1 binds to Procollagen and assists BMP1, increasing the Type-I Procollagen C-Terminal processing activity 10-fold, although it does not directly activate the enzyme. BMP1 is produced in a number of different forms, with different C-terminal regions. PCOLE1 and 2 may be isoform- or tissuespecific enhancers, giving a degree of regulation to the activity of BMP1 in different circumstances. PCOLE1 is produced in tissues rich in Type-I collagen, such as skin, kidney and tendon. It is secreted into culture media by a wide range of cell types.

General readings

Liepinsh E. et al. (2003) J. Biol. Chem. 278:25982-25989.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCOLCE antibody

(0.3ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCOLCE antibody

WB Suggested Anti-PCOLCE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PCOLCE antibody

Western Blot analysis of PCOLCE expression in transfected 293T cell line (H00005118-T02) by PCOLCE MaxPab polyclonal antibody.

Lane 1: PCOLCE transfected lysate(49.39 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PCOLCE antibody

Human Liver; WB Suggested Anti-PCOLCE Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; PCOLCE antibody - middle region (ARP56378_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PCOLCE antibody

PCOLCE Rabbit IgG Hu P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

PCOLCE antibody

Western Blot: PCOLCE Antibody [NBP1-58045] - This Anti-PCOLCE antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Xen, Ze WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PCOLCE

PCOLCE Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PCOLCE (26-449, His-tag)

PCOLCE Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

PCOLCE (26-449, His-tag)

PCOLCE Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

PCOLCE

PCOLCE Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
10 µg / €380.00
  Abnova Taiwan Corp.

PCOLCE

PCOLCE Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PCOLCE 293T Cell Transient Overexpression Lysate(Denatured)

PCOLCE 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

PCOLCE overexpression lysate

PCOLCE overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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