PCSK9 antibody

Principal name

PCSK9 antibody

Alternative names for PCSK9 antibody

PCSK-9, NARC1, Proprotein convertase subtilisin/kexin type 9, Proprotein convertase PC9, Subtilisin/kexin-like protease PC9, Neural apoptosis-regulated convertase 1, NARC-1

SwissProt ID

P59996 (Rat), Q80W65 (Mouse), Q8NBP7 (Human)

Gene ID

255738 (PCSK9), 100102 (Pcsk9), 298296

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PCSK9 antibody

The Neural apoptosis-regualted convertase 1 (NARC1) is encoded by gene proprotein convertase subtilsin kexin Type 9 (PCSK9) and is known to play a role in cholesterol homeostasis and is implicated in the differentiation of cortical neurons.. Proprotein convertase subtilisin/kexin type 9 (PCSK9) causes a third form of autosomal dominant hypercholesterolemia. NARC1 along with other known apoptotic mediators (caspase-3 and death receptor 6) are co-regulated and their mRNA levels are induced in cerebellar granule neurons after apoptotic injury. The NARC1 have significant pro-apoptotic effects that are partially reversed by polycaspase inhibitor BAF (1). Loss of function mutations (c.43_44insCTG) in the PCSK9 gene caused an in-frame insertion in African American and Caucasian population is associated with the etiology of familial hypobetalipoproteinemia (FHBL). This inframe mutation in NARC1 gene is involved in lowering cholesterol in the general population (2). This suggests that the variants of PCSK9 found in FH influence the secretion of apoB-containing lipoproteins, providing an explanation for the marked increase in circulating LDL in heterozygous carriers.
NARC1 is a 692 amino acid protein, expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines and in Schwan cells. The NARC1 mRNA is abundantly expressed in yolk sac and fetal liver, but the highest protein levels are found in differentiated FOA-1 cells which also exhibit highest levels of LDL receptor levels. The cellular levels of NARC1 in ER and intermediate vesicular compartment are reduced by cholesterol and 25-Hydroxy-cholesterol and up-regulated in tandem with HMG-CoA reductase, synthase and LDLR in the presence of steroids (3). The NARC1 is processed in the ER and secreted in the plasma. The NARC 1 gene encodes a novel proteinase K family proteinase. The domain structure of rat Narc 1 resembles that of the subtilisin-like proprotein convertases (SPCs), except that rNarc 1 lacks the canonical P-domain of SPCs, retaining only the RGD motif as part of what might be a cryptically functioning P-domain. NARC1 undergoes autocatalytic intramolecular processing at the site LVFAQ/, resulting in the cleavage of its pro-segment and the generation of an active proteinase with a broad alkaline pH optimum and no apparent calcium requirement for activity (4).

General readings

1. Kwon HJ, Lagace TA, McNutt MC, Horton JD, Deisenhofer J, Molecular basis for LDL receptor recognition by PCSK9. Proc. Natl. Acad. Sci. U.S.A. 2008 Feb 105 (6): 1820-5.
PMID: 18250299

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PCSK9 antibody

  Rabbit Polyclonal Proprotein Convertase 9/PCSK9 Antibody  
Western Blot: PCSK9 Antibody [TA337207] - WB detection of PCSK9 partial recombinant protein by using PCSK9 antibody at a concentration of 0.5 ug/ml. Rabbit Purified Hu C, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PCSK9 (N-term) antibody

Western blot analysis of PCSK9 Antibody (N-term) (AP18147PU-N) in Jurkat cell line lysates (35ug/lane). PCSK9 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PCSK9 (164-175) antibody

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human PCSK9 with C-terminal MYC tag probed with AP22407PU-N (0.5 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP22407PU-N(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PCSK9 (214-228) antibody

HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human PCSK9 with C-terminal MYC tag probed with AP22408PU-N  (1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP22408PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PCSK9 (C-term) antibody

  Goat Polyclonal Antibody against PCSK9  
TA303007 staining (0.2µg/ml) of McA cell lysates: untransfected (1), transfected with wild type human pcsk9 (2), transfected with S127R human pcsk9 (3). Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London. Goat Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

PCSK9 - human gene knockout kit via CRISPR

PCSK9 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PCSK9 - human gene knockout kit via CRISPR

PCSK9 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PCSK9 - human gene knockout kit via CRISPR

PCSK9 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PCSK9 - human gene knockout kit via CRISPR

PCSK9 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pcsk9 - mouse gene knockout kit via CRISPR

Pcsk9 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pcsk9 - mouse gene knockout kit via CRISPR

Pcsk9 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pcsk9 - mouse gene knockout kit via CRISPR

Pcsk9 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pcsk9 - mouse gene knockout kit via CRISPR

Pcsk9 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PCSK9

  Recombinant protein of human proprotein convertase subtilisin/kexin type 9 (PCSK9)  
PCSK9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PCSK9

  Recombinant protein of secreted form of human proprotein convertase subtilisin/kexin type 9 (PCSK9)  
PCSK9 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PCSK9

  Purified recombinant protein of Human proprotein convertase subtilisin/kexin type 9 (PCSK9)  
PCSK9 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

PCSK9

  Purified recombinant protein of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), D374Y mutant.  
PCSK9 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

PCSK9

  Purified recombinant protein of Human proprotein convertase subtilisin/kexin type 9 (PCSK9)  
PCSK9 Human > 90 %
Preparation: .
Purity Detail: >90%, as determined by Coomassie stained SDS-PAGE.
CHO cells

Regular Price: 10 µg / €199.00

Special Price: 10 µg / €139.00

  OriGene Technologies, Inc.

ELISA Kits

Catalog No.    

Human PCSK9 ELISA Kit

Human PCSK9 ELISA Kit  
1 kit / €389.00
  OriGene Technologies, Inc.

Mouse PCSK9 ELISA Kit

Mouse PCSK9 ELISA Kit  
1 kit / €400.00
  OriGene Technologies, Inc.

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