PDCD4 antibody

Principal name

PDCD4 antibody

Alternative names for PDCD4 antibody

H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like

SwissProt ID

Q53EL6 (Human), Q61823 (Mouse), Q98TX3 (Chick), Q9JID1 (Rat)

Gene ID

27250 (PDCD4), 18569 (Pdcd4), 64031

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Frog, Xen (Xenopus), Bov (Bovine), Can (Canine), Por (Porcine), S. aureus (Staphylococcus aureus)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP)

Background of PDCD4 antibody

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A (4) and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

General readings

Yang,H.S., et al., (2006) Mol. Cell. Biol. 26 (4), 1297-1306

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDCD4 antibody

Immunohistochemistry: AM09026PU-N PDCD4 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 k4C1 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PDCD4 antibody

Immunohistochemistry: AM09026PU-N PDCD4 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 k4C1 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

PDCD4 (210-221) antibody


PDCD4 antibody Cat.-No AP32008PU-N staining of HeLa lysate at 0.1µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PDCD4 (C-term) antibody

Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDCD4 antibody

(1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDCD4 antibody

Western blot analysis of extracts from 293 cells, using PDCD4 (Ab-457) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDCD4 antibody

Western blot using affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). Lane 1 contains recombinant Pdcd4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The anti-Pdcd4 antibody was used at a 1: 5,000 dilution. Personal Communication. M Young & A Jansen, NCI, Bethesda, MD. Rabbit Aff - Purified Hu, Ms, Rt, S. aureus, Xen C, E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDCD4 antibody

PDCD4 Rabbit IgG Purified Hu, Ms, Rt, Xen E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDCD4 antibody

WB using anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF (lane 2). No reactivity is seen for unstimulated (non-phosphorylated) NIH 3T3 cells (lane 1). The membrane was probed with the primary antibody at a 1:2,000 dilution, overnight at 4?. For detection HRP conjugated Rb-a-Mouse IgG was used at a 1:20,000 dilution in blocking buffer for 1 h at 4?. Mouse 9G6 Purified Hu, Ms, Rt, Xen E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDCD4 antibody

Western Blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Lane 1: Pdcd4 recombinant protein. Lane 2: none. Load: 100 ng per lane. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Pdcd4 phospho S457. Other band(s): Pdcd4 phospho S457 splice variants and isoforms Mouse 9G6 Purified Hu, Ms, Rt, Xen E, IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDCD4 antibody

Western blot analysis of PDCD4  expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDCD4 pSer457 antibody

This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA Mouse IgG1 9G6 Purified Hu, Ms, Rt, Xen C, E, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

PDCD4 pSer457 antibody

This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA Mouse IgG1 9G6 Purified Hu, Ms, Rt, Xen C, E, P, WB
25 µl / €190.00
  Acris Antibodies GmbH

PDCD4 antibody

PDCD4 Rabbit IgG Purified Hu, Ms, Rt, Xen E, WB
50 µg / €330.00
  Acris Antibodies GmbH

PDCD4 antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PDCD4 pSer457 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Breast. Rabbit Aff - Purified Frog, Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PDCD4 (C-term) antibody

Figure 1. Staining PDCD4 in Human Skin tissue by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Frog, Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PDCD4 pSer457 antibody

Immunohistochemistry. Affinity purified anti-Pdcd4 pS457 antibody was used at 1.25 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Rabbit IgG Aff - Purified Hu, Ms, Rt, Xen E, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

PDCD4 antibody

Immunohistochemistry analyzes of PDCD4 antibody (Cat.-No.: AP20338PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PDCD4 Control Peptide

PDCD4
50 µg / €160.00
  Acris Antibodies GmbH

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