PDE10A antibody

Principal name

PDE10A antibody

Alternative names for PDE10A antibody

Phosphodiesterase 10A, HSPDE10A, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A

SwissProt ID

Q8CA95 (Mouse), Q9QYJ6 (Rat), Q9Y233 (Human)

Gene ID

10846 (PDE10A), 63885 (Pde10a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of PDE10A antibody

Cyclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP and cGMP in to AMP and GMP respectively are phosphodiesterase type 10. Only one member of the cAMP and cGMP-specific phosphodiesterase type-10 (PDE10) family is cloned and characterized. The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and or use of alternate initiation sites (1). The PDE10 gene was first cloned from fetal human lung tissue library using bioinformatics approach (2). The deduced amino acid sequence contains 779 amino acids (MW 93-95 kDa) including a putative cGMP binding domain near the N-terminal region of the protein. The catalytic portion is 16-47% identical to other PDEs and PDE10 hydrolyze cAMP and cGMP with Km 0.26 and 7.2 uM with Vmax for cGMP twice as much that for cAMP. The presence of cAMP inhibits the hydrolysis of cGMP and vise versa. PDE10A is widely expressed in putamen and caudate nucleus in the brain, thyroid and testis. Surprisingly the expression of PDE10A was also abundant in Heart (see figure 1). PDE10 subtype has at least 6 subtype variants (PDE10A2, A3, A11, A12, A13 and A14) produced by either use of alternate initiation codon or by alternate splicing. PDE10A12 is a 883 amino acid long protein with unique N-terminal sequences and a catalytic domain that is common to all PDE10A variants. Long term potentiation-associated up-regulation of some PDE10A variants is observed by differential amplification of intron-containing transcripts of the PDE10A variants.

N-terminal splice variants of PDE5A (PDE5A1 and A2) possessing unique N-terminal sequences are isolated and characterized, these variants though have similar enzymatic activities and inhibitory properties but their tissue expression patterns are vastly different. Such observations suggest that unique N-terminal transcripts of PDEs may be specifically regulated by certain transcription factors. It has been shown that PDE10A has similar splice variants with transcripts (PDE10A2 and PDE10A12) with varying length of unique N-terminal ends. The tissue distribution of PDE10A2 is confined to placenta, kidney, brain and to less extend in other tissues. The expression in fetal tissue was in brain , heart and kidney beside minor expression in other tissues (1). The PDE10A2 has a unique protein kinase A phosphorylation site (T-16), this site was not phosphorylated in PDE10A1 variant (1).

General readings

General / background references:Kotera J et al. Subcellular localization of cyclic nucleotide phosphodiesterase type 10A variants, and alteration of the localization by cAMP-dependent protein kinase-dependent phosphorylation. J Biol Chem 279:4366-75 (2004). PubMed PMID: 14604994
Fujishige K et al. Striatum- and testis-specific phosphodiesterase PDE10A isolation and characterization of a rat PDE10A. Eur J Biochem 266:1118-27 (1999). PubMed PMID: 10583409

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE10A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PDE10A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500625, Dilution 1:50) Mouse IgG2b OTI1C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE10A antibody

Immunohistochemistry-Paraffin: PDE10A Antibody (1C9) [NBP1-47909] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PDE10A mouse monoclonal antibody. (Dilution 1:50) Mouse 1C9 Aff - Purified Hu P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

PDE10A antibody

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500635, Dilution 1:50) Mouse IgG1 OTI1B2 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PDE10A antibody

Anti-PDE10A mouse monoclonal antibody (TA500638) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE10A(RC211181). Mouse IgG2b OTI3G9 Purified Hu F, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE10A antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A (RC211181, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A. Mouse IgG1 OTI4A10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE10A antibody

Figure 1. Western blot using AP00661SU-N (1/20,000) against lysate from COS cells transfected with the indicated Human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE10A Control Peptide

PDE10A
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE10A antibody

Western blot using AP10255PU-N (1:500) and various
rat brain regions. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE10A control peptide

PDE10A
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE10A pThr16 antibody

PDE10A Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €490.00
  Acris Antibodies GmbH

PDE10A control peptide

PDE10A
0.1 mg / €250.00
  Acris Antibodies GmbH

PDE10A (91-110) antibody

PDE10A Rabbit Aff - Purified Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE10A control peptide

PDE10A
0.1 mg / €250.00
  Acris Antibodies GmbH

PDE10A antibody

PDE10A Rabbit Aff - Purified Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE10A antibody

PDE10A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PDE10A antibody

Detection limit for recombinant GST tagged PDE10A is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 4E1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDE10A antibody

PDE10A Rabbit Hu, Ms, Rt C, IP, WB
50 µl / €560.00
  Abnova Taiwan Corp.

PDE10A antibody

PDE10A Antibody Rabbit Serum Hu, Ms, Rt IP, P, WB
50 µl / €400.00
  Immuquest

PDE10A (Isoform PDE10A2) antibody

PDE10A Rabbit IgG Aff - Purified Hu, Ms, Rt WB
25 µg / €230.00
  Novus Biologicals Inc.

PDE10A antibody

Western Blot: PDE10A Antibody [NB300-645] - Western Blot of PD10A-101AP (1:500) using CNS Blot. 1. Frontal Cortex 2. Pariental Cortex 3. Striatum 4. Amygdela 5. Hypothalamus 6. Hippocampus 7. Olfactory bulb 8. Brain Stem 9. Cerebellum 10. Spinal cord and 11. Whole Brain. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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